Division of Main Geological and Engineering Controlling Types of Low-Production Coalbed Methane Well-Blocks and their Stimulation Measures.

At present, the identification of the main factors controlling low-production coalbed methane (CBM) wells and production enhancement measures does not consider geological and reservoir differences between individual wells and the CBM production effect of well groups simultaneously. In view of this, an evaluation index system suitable for use on inefficient well-blocks was established, which involves their geological resource potential, ground stress, coal structure, and comprehensive permeability under engineering influences from a systemic perspective. The main geological engineering types were determined by zoning superposition, and the corresponding stimulation measures were proposed. The evaluation results of the Shizhuang South Block in the Qinshui Basin show that the area of interest can be divided into Class I, II, and III potential areas, of which the Class II potential areas are subdivided into six blocks. In Zone I, the horizontal well infill method is preferred to increase the CBM production from well groups. At the current level of technology, it is difficult to gain economic benefits from Zone III. In Zone II, these stimulation measures such as the infilling method of staged fracturing horizontal wells, refracturing under supplementary injection holes, roof fracturing, the combined method of acidification and secondary fracturing or nitrogen injection and secondary fracturing, the combined method of acidification and controlled displacement secondary fracturing, and controlled displacement repetitive roof fracturing, among others, can be adopted.

Octahydroindolizine alkaloid Homocrepidine A from Dendrobium crepidatum attenuate P. acnes-induced inflammatory in vitro and in vivo.

ETHNOPHARMACOLOGICAL RELEVANCE: Dendrobium crepidatum Lindl. ex Paxton is a perennial epiphyte of Dendrobium genus, distributed in southern China, and utilized as the traditional Chinese medicine "Shihu" in Yunnan Province. Due to its heat-clearing and detoxicating properties, it is formulated as the "XiaoCuoWan" as recorded in the China Pharmacopoeia, and specially used to treat chronic skin inflammatory diseases, such as acne. AIM OF THE STUDY: This research aimed to estimate impact of the octahydroindoline alkaloid Homocrepidine A (HCA), isolated from D. crepidatum, on acne inflammation using both human THP-1 cells and mouse models. Furthermore, the potential anti-inflammatory mechanism of HCA has been analyzed through molecular biology methods and computer simulation. MATERIALS AND METHODS: THP-1 cells and mouse models induced by live Propionibacterium acnes (P. acnes) were employed to evaluate the anti-inflammatory properties of crude extract of D. crepidatum (DCE) and HCA. ELISA was utilized to detect the release of inflammatory cytokines in both cellular and murine ear tissues. RNAseq was used to screen the pathways associated with HCA-mediated inflammatory inhibition, while Western blot, RT-qPCR, and immunofluorescence were utilized to detect the expression of relevant proteins. Additionally, molecular docking simulations and cellular thermal shift assays were employed to confirm the target of HCA. RESULTS: Our research shows that DCE and HCA can effectively alleviate acne inflammation. HCA inhibits TLR2 expression by interacting with amino acid residues in the TIR domain of hTLR2, including Pro-681, Asn-688, Trp-684, and Ile-685. Moreover, HCA disrupts inflammatory signal transduction mediated by MAPK and NF-κB pathways through MyD88-dependent pathway. Additionally, HCA treatment facilitates Nrf2 nuclear translocation and upregulates HO-1 expression, thereby inhibiting NLRP3 inflammasomes activation. In vivo experiments further revealed that HCA markedly attenuated erythema and swelling caused by P. acnes in mice ears, while also decreasing the expression of pro-inflammatory cytokines IL-1ß and IL-8. CONCLUSIONS: Our research highlights the protective effects of D. crepidatum and its bioactive compound HCA against acne inflammation, marking the first exploration of its potential in this context. The discoveries indicate that HCA treatment may represent a promising functional approach for acne therapy.

Optimization of the Layer Location of Extraction Wells Based on the Unpressurized Gas Transport Law.

Protective layer mining is one of the most effective measures to control outbursts of coal seam gas in coal mines. Accurately grasping the overlying rock movement and pressure-relief gas migration patterns under protective layer mining conditions is a prerequisite for efficient surface coalbed methane extraction; it is the basis for green emission reduction in coal mines. A physical model was established using the Ji15-33200 working face of Pingmei Shi Mine as the research object, and a method combining theoretical calculation and numerical simulation was used to obtain the overlying rock movement. In situ stress distribution characteristics of the stope after the upper protective layer was mined to explore the upper protective layer migration rules of pressure-relief gas after mining. On this basis, the location and layer of surface coalbed methane production wells was determined. The research results show that the coal and rock formations on the floor of the goaf experienced a deformation process of compression → expansion → rebalance during the mining process; the stress changes of the overlying and underlying coal strata in the goaf have experienced a process of increasing → decreasing → rebalance; and gas migrates upward through the fissure zone in the coal layer and slowly diffuses in other microfissure areas. When the pressure reaches a certain value, it is enriched in the crack development area and the upper part of the fissure zone; combined with the relevant geological conditions of the study area, it was determined that the upper part of the roof of the Ji16-17 coal seam is a gas-rich area. By comparing three mining vertical wells at different positions in the horizontal direction, it was found that the extraction effect was significant in the "O″ ring, near the excavation face.

Effect of bioturbation of the mitten crab on distribution of tire wear particles and their combined effect on sediment ecosystem.

Tire wear particles (TWPs) are a major source of environmental microplastic pollution which gradually settle and accumulate in sediments after entering the aquatic environment, which can affect the behaviors of benthic organisms. Bioturbation of benthic species could affect the fate, impacts and potential risks of TWPs by altering the properties and structure of sediments. Therefore, in this study, the effect of TWPs on the burrowing activity of Chinese mitten crab (Eriocheir sinensis) was investigated. In addition, the effects of crab bioturbation on the distribution of TWPs and their additives were studied. The combined effects of TWPs and crab bioturbation on the microbial communities in the sediments were also explored. The results of this study showed that both TWPs and the leachate significantly inhibited the burrowing activity of crabs. TWPs in the surface layer of sediments were re-distributed by crab bioturbation and enriched mainly in the sediments near the burrow walls. Meanwhile, the heavy metals (i.e., Zn, Ca, Mg, Ba and Al) used as additives during the tire production in the burrow walls significantly increased as the accumulation of TWPs near burrow walls. In this study, TWP exposure decreased the bacterial diversity and abundance, as well as the functional genes related to carbon and nitrogen cycling process, but crab bioturbation increased them in the sediments of burrow walls by constructing a unique habitat. However, after TWPs entering into burrows, they were significantly decreased in the sediments near the burrow walls like the effects of TWPs, suggesting the negative effects of TWPs could play a dominant role in this combined system. Overall, this study is important for evaluating the distribution and effects of TWP pollution in the sediment ecosystem under biological factors such as bioturbation.

Experimental Studies on the Crack Initiation and Propagation of Hydraulic Fracturing with Different Coal Structure Combinations.

Coal structure is one of the key geological factors that affects the effect of coal reservoir stimulation. Based on the geological spatial combination characteristics, thickness, and proportion of different coal structures, the coal reservoir is divided into different coal structure combination types. The hydraulic fracturing device is used to carry out indoor fracturing experiments and dissect the crack initiation and expansion characteristics with different coal structure combinations. The results show that the coal structure combination is of the binary type (undeformed coal + granulated coal or cataclastic coal + granulated coal), and the undeformed coal (cataclastic coal) can overcome the tensile strength and minimum principal stress when it is driven by the high-pressure fluid. The undeformed coal (cataclastic coal) ruptures and forms longitudinal cracks. The increasing proportion of granulated coal inhibits crack expansion and promotes the transverse deformation of coal. The interface contact point between the undeformed coal (cataclastic coal) and granulated coal easily fractures along the cross section of the specimen. When the coal structure combination is the triplex type (undeformed coal + granulated coal + cataclastic coal or cataclastic coal + granulated coal + cataclastic coal), the undeformed coal or cataclastic coal is transformed. The forming fractures in the undeformed coal (cataclastic coal) can cut through the soft coal when the thickness of the undeformed coal (cataclastic coal) is large and the thickness of granulated coal is thin. On the contrary, it is not easy to cut through. When the coal structure combination is granulated coal + cataclastic coal + granulated coal, the cataclastic coal fails under shear stress and forms the crack along the cross section of the coal sample. The granulated coal inhibits the crack expansion at both ends. The research results have an important indicative significance for further understanding the fracture initiation and propagation mechanism of hydraulic fracturing with complex coal structures in coal reservoirs.

Toxic effects of tire wear particles and the leachate on the Chinese mitten crab (Eriocheir sinensis).

Tire wear particles (TWPs) were considered as an important component of microplastic pollution in the aquatic environment. To understand the ecotoxicity of TWPs to crustacean, this study investigated toxic effects of TWPs and the leachate on the mitten crab Eriocheir sinensis and the accumulation of TWPs in the crabs. Although TWPs could be accumulated in various tissues (i.e., liver, gills and gut) of the crabs, exposure to TWPs or the leachate had no lethal effect on the crabs in this study. Lower concentrations of TWPs and the leachate exposure could stimulate the antioxidant defense system of the crabs, while higher concentrations could disrupt the stress defense system. In addition, the energy supply and metabolism of the crabs could also be affected by TWPs or the leachate. The transcriptomic profiles showed that the toxic mechanisms of TWPs and the leachate were not exactly the same. Similar to the results of biochemical analysis, several Gene Ontology (GO) terms and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways related to oxidative stress and energy metabolism were significantly regulated by both TWPs and the leachate. However, TWPs could affect the expression of genes enriched in immune-related pathways, while the leachate regulated the enrichment of some other signaling pathways including FoxO signaling pathway, insulin signaling pathway, RIG-I-like receptor signaling pathway, NOD-like receptor signaling pathway, PPAR signaling pathway and neuroactive ligand-receptor interaction. Overall, our study could provide basic biological information for assessing the ecological risk of the TWP pollution in the aquatic environment and was useful to understand the potential toxic mechanisms of the TWPs and the leachate to crustaceans.

Toxicity of tire wear particles and the leachates to microorganisms in marine sediments.

Tire wear particles (TWPs), which are among the microplastic pollutants in the environment, can inevitably accumulate in coastal sediments. The present study comprehensively investigated the effect of pristine TWPs on bacterial community structure in coastal sediments and compared the effect of pristine TWPs and aged TWPs on nine strains of bacteria in sediments. In addition, the effect of the TWP leachate was studied with all the nine bacterial strains and the toxicity-causing substances in the leachate was investigated using Bacillus subtilis. Exposure to TWPs could lead to a shift in bacteria community and affect nitrogen metabolism in marine sediments. Aged TWPs were more toxic than pristine TWPs due to changes in particle surface characteristics. The leachate exhibited greater toxicity than TWPs as well, and Zn was identified to be the major toxicity-causing substance. The overall results of this study are important for understanding the effects of TWPs and the leachates on microorganisms in marine sediments.

An optimal selection method of wells for secondary fracturing in a single coal seam and its application.

At present, methods including mathematical modeling, physical or numerical simulation, and in-situ monitoring have been generally adopted to determine evaluation parameters for coalbed methane (CBM) wells for secondary fracturing. These conventional methods either entail many assumptions, or some parameters are difficult to obtain, resulting in a certain deviation between the evaluation results and reality, or the application cost is high, preventing the monitoring of each CBM well. In view of this, an evaluation index system for the gas production potential, effective length of cracks formed by fracturing, and supporting length of proppant in cracks was established based on the system theory. The evaluation indices were characterized through production data, such as logging, fracturing and drainage, which could avoid potential bias in evaluation when only considering a certain parameter and ensured accuracy and practicability of the evaluation parameters for each well. Principal component analysis (PCA) and the entropy weight method (EWM) were used to obtain weights of evaluation parameters, which avoided the contradiction of contributions of various parameters to optimal selection and the rationalized results. In this way, a method for step-wise optimal selection of wells for secondary fracturing integrating construction of evaluation parameters, determination of critical values, and entropy evaluation was proposed. The results of an evaluation of the Shizhuang South Block of Qinshui Basin (Shanxi Province, China) indicate that wells whose three evaluation indices are satisfied are most preferable; wells that only meet the effective length of cracks formed by fracturing or effective supporting length of proppant in cracks can be selected; wells which do not meet the gas production potential or all of the three parameters cannot be selected.

Geochemistry and Organic Petrology of Middle Permian Source Rocks in Taibei Sag, Turpan-Hami Basin, China: Implication for Organic Matter Enrichment.

The Taodonggou group of Middle Permian is an important source rock in Taibei sag of Turpan-Hami basin. Due to its deep burial, drilling has only been revealed in recent years. Based on organic petrology and organic geochemistry experiments, this paper studies the organic petrology, organic geochemistry, sedimentary environment, and hydrocarbon generation potential of source rocks in Taibei sag, Turpan-Hami basin, and reveals the influence of the sedimentary environment on the organic matter abundance of source rocks. The results are as follows: (1) The organic matter of the Middle Permian source rocks in Taibei sag of Turpan-Hami basin is mainly sapropelite and exinite. The vitrinite is mainly vitrodetrinite, and the exinite is mainly lamalginiite. (2) The total organic carbon content value is 0.55-6.08 wt %, and the average value is 2.58 wt %. The PG value ranges from 0.78 mg HC/g to 30.86 mg HC/g, and the average value is 4.88 mg HC/g. Chloroform asphalt "A" is 0.046-0.8767 wt %, and the average value is 0.285 wt %. The types of organic matter are mainly III and II-III, and the R o value is 0.628-1.49 wt % (average = 0.988 wt %). The T max distribution is 329-465 °C. The average temperature is 434.7 °C, which is in the mature stage (oil window stage). The Middle Permian source rocks are mainly very good to excellent source rocks with a good hydrocarbon generation potential. (3) The source rocks are deposited in a semihumid and semiarid climate. Organic matter is input as a mixed source. The early and late stages is dominated by terrestrial higher plants. The middle stage is dominated by lower aquatic organisms, and the sedimentary environment consists of weak reduction and weak oxidation environments. (4) In the study area, the abundance of organic matter has a weak negative correlation with CPI and a positive correlation with Pr/Ph and ∑C21-/∑C22+. Under the coaction of paleoclimate, organic matter input, and redox environment, the enrichment model of organic matter with high productivity and weak oxidation environment characteristics can also form excellent source rocks. This study is of great significance and provides theoretical guidance for the exploration of deep oil and gas resources.

The adsorptive behaviour of kaolinite to sodium dodecyl benzene sulphonate and the structural variation of kaolinite.

Analysis of the adsorptive behaviour of kaolinite to sodium dodecyl benzene sulphonate (SDBS) at different concentrations can provides a basis for selecting the best concentration. The adsorptive capacity and adsorptive behaviour of kaolinite to SDBS at different concentrations were studied using ultraviolet spectrophotometer, pseudo-first-order adsorption kinetics model, and pseudo-second-order adsorption kinetics model. Scanning electron microscopy with energy dispersive spectrometry (SEM-EDS), X-ray diffraction (XRD), and infrared spectroscopy (FTIR) were used to study the variation characteristics of surface structure, crystallinity indices, and main functional groups on kaolinite before, and after, adsorption. The results show that as the SDBS concentration increase, the adsorptive capacity of kaolinite to SDBS increase. The adsorption process can be accurately fitted by the pseudo-secondary adsorption kinetic model, which means the adsorptive behaviour was mainly chemical in origin. The adsorption of SDBS by kaolinite mainly occurs on the surface. The solidification, lamellar aggregation, and crystallinity index of kaolinite are more obvious after the adsorption of SDBS, but the interlayer spacing of kaolinite did not change to any significant. After the adsorption of SDBS, the intensity ratio of 1000-1008 cm-1 bands changed significantly, indicating the change of the chemical environment, and the adsorptive behaviour was chemical.

Effects of inulin diet supplementation on production performance, gut traits, and incidence of ascites in Haidong chicks under hypoxic conditions.

OBJECTIVE: Effects of inulin supplementation in diet of Haidong chicks under hypoxic conditions on production performance, intestinal morphologic change, microflora contents and the incidence of ascites were studied. METHODS: Commercial male chicks (360) were randomly divided into 6 groups and were fed diets supplemented with 0, 0.05, 0.075, 0.1, 0.125, and 0.15 g/kg of inulin, respectively. RESULTS: The body weight gain and feed intake were improved in chicks fed the diets supplemented with 0.1 and 0.125 g/kg of inulin, from d 1 to d 42 (p<0.05); moreover, blood parameters were positively affected when inulin was included in the diets and the thickness of the intestinal wall and muscle tissue in duodenum, jejunum, and ileum tended to increase (p<0.05), and the villi height and crypt depth in duodenum, jejunum, and ileum (p<0.05). Regarding the number of goblet cells in duodenum, jejunum and ileum tended to increase when chicks were fed the diets supplemented with 0.075, 0.1, 0.125, and 0.15 g/kg (p<0.05) of inulin. When chicks were fed diets supplemented with 0.75 or 0.1 g/kg of inulin, a significant reduction of Escherichia coli counts in the cecum was observed; for a contrary, a significant increment of Bifidobacterium and Lactobacillus was observed in cecum and ileum. Finally, supplementing the feed with inulin determined an overall reduction of ascites incidences in comparison to the control group. CONCLUSION: Thus, the results observed in the present study clearly suggest that the diet supplementation with a quantity of inulin ranging between 0.1 and 0.125 g/kg, can improve growth performances, intestinal morphology, internal microbial balance and ascites incidence, in broiler chicks raised at high altitude area. Even though these findings may be of interest for the poultry industry, they may particularly be relevant in those areas characterized by high altitude such as Northwest China regions.

Nanoscale Pore Structure Characteristics of Deep Coalbed Methane Reservoirs and Its Influence on CH4 Adsorption in the Linxing Area, Eastern Ordos Basin, China.

The high gas content of deep coal seams is a driving force for the exploration and development of deep coalbed methane (CBM). The nanoscale pores, which are the main spaces for adsorption and storage of CBM, are closely related to the burial depth. Based on integrated approaches of vitrinite reflectance (Ro), maceral composition, scanning electron microscope (SEM), proximate analysis, fluid inclusion test, low-temperature N2 adsorption-desorption, and CH4 isothermal adsorption, the nanoscale pore structure of coals recovered at depths from 650 to 2078 m was determined, and its influence on the CH4 adsorption capacity was discussed. The results show that the coal rank has a good linear relationship with the current burial depth of the coal seams; that is, the influences of the burial depth on the coals can be reflected by the influences of the coal rank on the coals. With the increase in the coal rank, the moisture and volatile content decrease, and the fixed carbon content increases. The variation in the pore volume and specific surface area with the increase in the coal rank can be divided into two stages: the rapid decline stage (when 0.75% 1.0%, the FD of the micropores is close to 2. This indicates that with the increase in the degree of coalification, the surface of the micropores is simpler. The above results show that the gas adsorption capacity of coal first slightly decreases (when 0.75% < Ro < 1.0%) and then increases (when 1.0% < Ro < 1.35%), and the coincident results are shown in the Langmuir volume (VL) test results.

The effect of Aspergillus niger as a dietary supplement on blood parameters, intestinal morphology, and gut microflora in Haidong chicks reared in a high altitude environment.

AIM: The effects of the inclusion of Aspergillus niger in the diet of Haidong chicks reared in the Qing-Zang high altitude area (China) under hypoxic conditions. MATERIALS AND METHODS: A total of 720 Haidong chicks were randomly divided into six groups and fed diets supplemented with 0%, 0.5%, 0.75%, 1.0%, 1.25%, and 1.5% of A. niger to determine blood parameters, intestinal morphology, and gut microflora in Haidong chicks reared in a high altitude environment. RESULTS: Packed cell volume (PCV), red blood cell (RBC) count, white blood cell count, and hemoglobin concentration increased in the groups fed diets containing A. niger. The administration of A. niger in 1.0% and 1.25% significantly decreased the concentration of Escherichia coli in the cecum, while the concentration of Bifidobacterium and Lactobacillus in the cecum and ileum was increased in the treated groups. When compared to the control groups, villi height, crypt depth, and goblet cell density in the intestine was raised, in general, in the groups treated with A. niger. CONCLUSION: These findings suggest that 1.25% A. niger as dietary supplement may improve the resistance to ascites among birds reared under hypoxic conditions.

AC/DC Fields Demodulation Methods of Resonant Electric Field Microsensor.

Electric field microsensors have the advantages of a small size, a low power consumption, of avoiding wear, and of measuring both direct-current (DC) and alternating-current (AC) fields, which are especially suited to applications in power systems. However, previous reports were chiefly concerned with proposing new structures or improving the resolution, and there are no systematic studies on the signal characteristics of the microsensor output and the demodulation methods under different electric fields. In this paper, the use of an improved resonant microsensor with coplanar electrodes, and the signal characteristics under a DC field, power frequency field, and AC/DC hybrid fields were thoroughly analyzed respectively, and matching demodulation methods derived from synchronous detection were proposed. We theoretically obtained that the frequencies of the detectable electric fields should be less than half of the resonant frequency of the microsensor, and that the sensitivities of the microsensor were identical for AC/DC hybrid fields with different frequencies. Experiments were conducted to verify the proposed demodulation methods. Within electric field ranges of 0-667 kV/m, the uncertainties were 2.4% and 1.5% for the most common DC and 50 Hz power frequency fields, respectively. The frequency characteristic test results of the microsensor were in agreement with those of the theoretical analysis in the range of 0-1 kHz.

Classification of Pore-fracture Combination Types in Tectonic Coal Based on Mercury Intrusion Porosimetry and Nuclear Magnetic Resonance.

Differences in content, distribution, and connectivity of pores and fractures with different sizes in coal lead to different modes of gas migration. An accurate classification of pore-fracture combination types in coal can lay a foundation for studying gas migration. High-pressure mercury intrusion and nuclear magnetic resonance (NMR) experiments were conducted on coal samples collected from the Changping coal mine in Jincheng City, Shanxi Province, and Pingdingshan no. 4 mine in Pingdingshan City, Henan Province, China. The fractal dimensions of pores with different sizes were calculated using the Menger model. By combining the data with T2 spectra obtained by NMR, critical values for distinguishing diffusion pores from seepage pores-microfractures were determined. In addition, the main parameters affecting development of diffusion pores and seepage pores-microfractures and pore-fracture connectivity were analyzed, and a comprehensive evaluation index system for pores and fractures was established by selecting eight indices. Based on the method combining the analytical hierarchy process with multiparameter superposition, a method for determining critical values, establishing the evaluation index system, and classifying pore-fracture combination types was formed. The pore-fracture combination types in the test coal samples were classified according to the experimental data. The results indicate that the critical values for distinguishing diffusion pores from seepage pores-microfractures based on fractal dimensions obtained through mercury intrusion porosimetry and T2 spectra obtained by NMR are 72 nm and 2.5 ms, respectively. The studied coal samples can be classified into three combination types, separately characterized by high diffusivity and permeability and poor pore-fracture connectivity; low diffusivity, high permeability, and good pore-fracture connectivity; and low diffusivity and permeability and good pore-fracture connectivity. In the coal samples from the Changping coal mine, diffusion pores and seepage pores-microfractures are developed, while the connectivity between pores and fractures is poor. The coal samples from Pingdingshan no. 4 mine have undeveloped diffusion pores and seepage pores-microfractures but good connectivity between pores and fractures. The research results provide a method for classifying pore-fracture combination types in coal samples taken from different regions.

The GLP-1 agonist, liraglutide, ameliorates inflammation through the activation of the PKA/CREB pathway in a rat model of knee osteoarthritis.

BACKGROUND: Inflammation is a common pathological phenomenon of osteoarthritis (OA). Accumulated evidence indicates that ameliorating or suppressing inflammation might be a promising and effective therapeutic strategy for the treatment of OA. Notably, glucagon-like peptide-1 (GLP-1)-based drugs are being successfully used to control glucose levels in patients with diabetes mellitus. In addition, recent findings have indicated that GLP-1 agonists, such as liraglutide have therapeutic potential in preventing inflammation-related disorders through the regulation of protein kinase A (PKA)/ cyclic adenosine monophosphate (cAMP) response element-binding protein (CREB) signals. Intra-articular injection of monoiodoacetate (MIA) has been widely used to induce OA. Thus, the present study aimed to investigate whether liraglutide has anti-inflammatory effects on MIA-induced OA rats and uncover its underlying molecular mechanisms. METHODS: Intra-articular injection of MIA was used to induce knee OA in a rat model. Subcutaneous injection of liraglutide was used to upregulate the expression of GLP-1 receptor (GLP-1R). Western blot analysis was utilized to measure the expression of GLP-1R, PKA/CREB pathway components and inflammation-related proteins, such as tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1ß), and IL-6. Immunoprecipitation techniques were used to detect the interactions between GLP-1R and the PKA/CREB pathway. RESULTS: The levels of GLP-1R decreased significantly in the knees of OA rats, accompanied by the downregulation of PKA /CREB signals and upregulation of inflammation-related proteins. We also found that GLP-1R interacted with the PKA/CREB pathway and that liraglutide could activate PKA/CREB signals, thereby inhibiting the expression of inflammation-related proteins. CONCLUSIONS: Together our results suggest that liraglutide exhibits anti-inflammatory activity through the activation of the PKA/CREB pathway in an OA rat model.

Adsorption Characteristics of Anionic Surfactant Sodium Dodecylbenzene Sulfonate on the Surface of Montmorillonite Minerals.

The adsorption characteristics of sodium dodecylbenzene sulfonate (SDBS) on the surface of montmorillonite can lay a foundation for obtaining the optimum concentration of the anionic surfactant. The best absorption wavelength of SDBS was determined using an ultraviolet spectrophotometer. The standard curves of concentration and absorbance of SDBS were established. The amount of SDBS adsorbed on the surface of montmorillonite at various concentrations was calculated by stirring adsorption method. Scanning electron microscopy-energy dispersive X-ray spectrometry (SEM-EDS), X-ray diffraction (XRD), zeta potentiometer, and Fourier transform infrared (FTIR) spectroscopy were used to observe the changes of the structure, main ions, interlayer spacing, potential, and main functional groups on the montmorillonite surface before, and after, adsorption. The test results of SEM with EDS (SEM-EDS) showed that the surface of the montmorillonite after SDBS adsorption was rougher, and the adsorption capacity of the surface was enhanced as the SDBS concentration increased. The XRD results indicated that SDBS adsorbed on the interlayer of montmorillonite repulsed interlayer water and reduced the interlayer water content. With the increase of SDBS concentration, the interlayer spacing of the montmorillonite available for adsorbing SDBS decreased further. Additionally, interlayer adsorption and surface adsorption exist simultaneously in montmorillonite in SDBS solution. The distribution of total adsorption capacity of SDBS in the layers and on the surface of montmorillonite accords with the adsorption result simulated by a pseudo-second-order kinetic model. The increase in concentration of SDBS adsorbed by montmorillonite is the main reason for the decreased initial adsorption rate. The zeta potential test showed that the addition of H+ to the SDBS solution could reduce electrostatic repulsion and promote the adsorption of SDBS on montmorillonite. The results of this study provide an experimental basis for the study of the mechanism of SDBS adsorption on montmorillonite.