Loganin reduces neuroinflammation and promotes functional recovery after spinal cord injury through inhibiting NF-κB/NLRP3 signalling.

The highly disabling nature of spinal cord injuries (SCI) and high cost of treatment and rehabilitation impose a burden on families and society. Loganin has potential medicinal value in alleviating neuroinflammation. This study aimed to explore whether loganin can be used to reduce SCI-induced neuroinflammation and elucidate the molecular mechanisms underlying its action. An SCI rat model was developed to assess whether loganin promotes motor recovery after SCI. The anti-inflammatory effects of loganin on the dorsal horn of the spinal cord were identified by haematoxylin-eosin and immunohistochemical staining. The inflammatory effects of loganin were characterised using a lipopolysaccharide (LPS)-induced neuroinflammatory model in BV2 cells. For mechanistic exploration, the signalling pathways and target proteins of loganin action were predicted using bioinformatics and computational biology and then validated in cellular inflammation models. Loganin promoted animal motor recovery after SCI at the behavioural level, and it inhibited M1 differentiation of microglia and reduced NOD-like receptor thermal protein domain associated protein 3 (NLRP3)-mediated inflammatory responses at the tissue level. Loganin inhibited LPS-induced inflammation in BV2 cells, and bioinformatics and computational biology suggested that loganin acts on the p65 protein through the nuclear factor kappa-B (NF-κB)/NLRP3 signalling pathway. This was validated in a cellular model in which p65 trans-overexpression eliminated the downregulation of inflammatory factors by loganin. In conclusion, loganin reduces neuroinflammatory responses and promotes motor recovery after SCI. Loganin inhibits the NF-κB/NLRP3 signalling pathway by targeting the p65 protein to achieve repair.

Research on the impact of bank comxpetition on stability-Empirical evidence from 4631 banks in US.

This paper systematically analyzes the impact mechanism of bank competition on stability. We select the balanced panel data of 4,631 non-failure banks from 2002 to 2017 released by FDIC to do the estimation and then make an appraisal on bank competition with Lerner index and two kinds of Z-score to measure bank stability respectively. The 2SLS with fixed effect is applied for estimation. Our results suggest that: (1) Competition is a living environment for all industries and bank competition mainly affects stability through franchise value, the cost of borrowing and operating behavior. (2)According to the overall regression, we find that there is an inverted U-shaped relationship between bank competition and stability with an inflection point where the Lerner index is about 0.35. Generally, the US banking industry has always been in a state of excessive competition during the observed period. The further analysis of 3 stages indicates that excessive bank competition, an 'invisible hand', may be one of the most important factors triggering the financial crisis. (3) According to the regression on regions, the inverted U-shaped relationship between bank competition and stability is also existing and the inflection point of Lerner index is between 0.3 and 0.37. However, there is also some regional heterogeneity in terms of the degree of competition, the level of stability and the ability to resist risks and maintain stability when facing competition. This paper may help financial regulators and commercial banks formulate differentiate regulatory policies and business strategies so that banks can control risks better and enhance stability in different competitive environments.

Effectiveness of sedative and analgesic bundle on delirium and clinical prognosis in mechanically ventilated patients / 中国实用护理杂志

Objective To research the intervention effect of the ABCDE Bundle in mechanically ventilated patients. Methods A prospective randomized controlled trail (RCT) was selected 84 mechanically ventilated patients aged 22-45 years in the ICU of respiratory and severe medical center of the affiliated hospital of Logistics University of PAP from January to December in 2016. The research samples were randomly divided into intervention group (n=42) and control group (n=42). Samples were chosen to compare the difference of basic situation, ICU length of stay, 28-day hospital survival rate and the incidence of delirium between the patients of intervention group and control group. Results The average ICU hospitalization time in the intervention group was (11.76± 5.15) days, while that in the control group was (17.64 ± 8.04) days, and the difference between the 2 groups was statistically significant (t=3.99, P<0.01). The 4-week survival rate and delirium incidence rate in intervention group were 90.48％ (38/42) and 66.67％ (28/42) respectively, while those in control group were 71.43％ (30/42) and 40.48％ (17/42) respectively, and the difference between 2 groups was statistically significant (χ2=4.94, 5.79, P<0.05). Conclusion The application of bundles of care strategy could reduce the incidence of delirium and improve the clinical outcome of mechanically ventilated patients.

Effects of Manganese Superoxide Dismutase 9 Ala/Val Genetic Polymorphisms on Coronary Heart Disease / 现代检验医学杂志

Objective To study associations between manganese superoxide dismutase 9 Ala/Val (Mn-SOD 9 Ala/Val)genet-ic polymorphism and total superoxide dismutase (T-SOD)and Mn-SOD activity and the impact on coronary heart disease (CHD)were studied.Methods There were 82 CHD patients and 57 controls in this research.Sequencer was used to identify the genotype of Mn-SOD 9 Ala/Val genetic polymorphism and colorimeter was used to detect the serum T-SOD and Mn-SOD activity.Results Compared with the control group,the serum T-SOD and Mn-SOD activity of the CHD group was significantly reduced(t=4.83,6.57,P all<0.05),while the VV genotype and V allele of Mn-SOD 9 Ala/Val genetic poly-morphism of the CHD group were higher (χ2 =4.75,P <0.05).The serum T-SOD and Mn-SOD activity of the Mn-SOD 9 VV genotype was significantly lower than the Mn-SOD 9 AA genotype(t=2.96,3.11,P all<0.05).Conclusion The ser-um T-SOD and Mn-SOD activity in the CHD patients was reduced.Mn-SOD 9 Ala/Val genetic polymorphism was involved in the pathogenesis of CHD by influencing the Mn-SOD activity.

PAD regimen for relapsed or refractory patients with multiple myeloma / 中华血液学杂志

<p><b>OBJECTIVE</b>To investigate the efficacy and safety of PAD [bortezomib (PS-341), doxorubicin and dexamethasone] regimen for relapsed or refractory multiple myeloma (MM).</p><p><b>METHODS</b>Seventeen patients with relapsed or refractory MM received two to four 21-day cycles of PAD: an intravenous bolus of bortezomib (1.3 mg/m2) on days 1, 4, 8, and 11; doxorubicin 10 mg per day on days 1 to 4, and dexamethasone 40 mg on days 1-4. Response was evaluated according to International Myeloma Working Group Criteria (IMWG 2006), toxicity was graded according to NCI CTCAE (common terminology criteria for adverse events) v 3.0.</p><p><b>RESULTS</b>After 2-4 courses of PAD, 14 patients (82.4%) response, including complete response (CR) in 4 (23.5%), very good partial response (VGPR) in 4 (23.5%), partial response (PR) in 6 (35.3%) and stable disease (SD) in 3 (17.6%). Median time to progression was 9.5 months. The median course to response was 1.6 (1-3). All of 5 patients with extramedullary plasmacytoma achieved at least PR after the first cycle therapy; the plasmacytoma disappeared after 1-2 cycles of PAD. The efficacy was independent of other prognostic factors such as beta2-MG. Adverse events included thrombocytopenia in 9 patients (52.9%), leukopenia in 4 (23.5%), peripheral neuropathy in 4 (23.5%), varicella herpes zoster in 3 (17.6%), fatigue in 6 (35.3%) and diarrhea in 2 (11.7%). All of these adverse reactions could be controlled with routine supportive treatment, only one patient died from respiratory failure during his fifth PAD cycle.</p><p><b>CONCLUSIONS</b>PAD regimen should be considered as an appropriate treatment for relapsed or refractory MM, especially for MM with extramedullary plasmacytoma. Its efficacy is independent of traditional prognostic factors. The side effects are usually manageable.</p>