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1.
Scanning ; 38(2): 148-63, 2016.
Article in English | MEDLINE | ID: mdl-26235517

ABSTRACT

Noise on scanning electron microscope (SEM) images is studied. Gaussian noise is the most common type of noise in SEM image. We developed a new noise reduction filter based on the Wiener filter. We compared the performance of this new filter namely adaptive noise Wiener (ANW) filter, with four common existing filters as well as average filter, median filter, Gaussian smoothing filter and the Wiener filter. Based on the experiments results the proposed new filter has better performance on different noise variance comparing to the other existing noise removal filters in the experiments.

2.
J Microsc ; 258(2): 140-50, 2015 May.
Article in English | MEDLINE | ID: mdl-25676007

ABSTRACT

A new technique based on cubic spline interpolation with Savitzky-Golay smoothing using weighted least squares error filter is enhanced for scanning electron microscope (SEM) images. A diversity of sample images is captured and the performance is found to be better when compared with the moving average and the standard median filters, with respect to eliminating noise. This technique can be implemented efficiently on real-time SEM images, with all mandatory data for processing obtained from a single image. Noise in images, and particularly in SEM images, are undesirable. A new noise reduction technique, based on cubic spline interpolation with Savitzky-Golay and weighted least squares error method, is developed. We apply the combined technique to single image signal-to-noise ratio estimation and noise reduction for SEM imaging system. This autocorrelation-based technique requires image details to be correlated over a few pixels, whereas the noise is assumed to be uncorrelated from pixel to pixel. The noise component is derived from the difference between the image autocorrelation at zero offset, and the estimation of the corresponding original autocorrelation. In the few test cases involving different images, the efficiency of the developed noise reduction filter is proved to be significantly better than those obtained from the other methods. Noise can be reduced efficiently with appropriate choice of scan rate from real-time SEM images, without generating corruption or increasing scanning time.

3.
Scanning ; 36(5): 530-9, 2014.
Article in English | MEDLINE | ID: mdl-25139061

ABSTRACT

An improvement to the previously proposed adaptive Canny optimization technique for scanning electron microscope image colorization is reported. The additional feature, called pseudo-mapping technique, is that the grayscale markings are temporarily mapped to a set of pre-defined pseudo-color map as a mean to instill color information for grayscale colors in chrominance channels. This allows the presence of grayscale markings to be identified; hence optimization colorization of grayscale colors is made possible. This additional feature enhances the flexibility of scanning electron microscope image colorization by providing wider range of possible color enhancement. Furthermore, the nature of this technique also allows users to adjust the luminance intensities of selected region from the original image within certain extent.

4.
Comput Biol Med ; 49: 46-59, 2014 Jun.
Article in English | MEDLINE | ID: mdl-24736203

ABSTRACT

A computer-aided detection auto-probing (CADAP) system is presented for detecting breast lesions using dynamic contrast enhanced magnetic resonance imaging, through a spatial-based discrete Fourier transform. The stand-alone CADAP system reduces noise, refines region of interest (ROI) automatically, and detects the breast lesion with minimal false positive detection. The lesions are then classified and colourised according to their characteristics, whether benign, suspicious or malignant. To enhance the visualisation, the entire analysed ROI is constructed into a 3-D image, so that the user can diagnose based on multiple views on the ROI. The proposed method has been applied to 101 sets of digital images, and the results compared with the biopsy results done by radiologists. The proposed scheme is able to identify breast cancer regions accurately and efficiently.


Subject(s)
Breast Neoplasms/diagnosis , Fourier Analysis , Image Interpretation, Computer-Assisted/methods , Magnetic Resonance Imaging/methods , Adult , Aged , Breast/pathology , Breast Neoplasms/pathology , Female , Humans , Imaging, Three-Dimensional/methods , Middle Aged
5.
J Microsc ; 253(1): 1-11, 2014 Jan.
Article in English | MEDLINE | ID: mdl-24164248

ABSTRACT

A new technique based on cubic spline interpolation with Savitzky-Golay noise reduction filtering is designed to estimate signal-to-noise ratio of scanning electron microscopy (SEM) images. This approach is found to present better result when compared with two existing techniques: nearest neighbourhood and first-order interpolation. When applied to evaluate the quality of SEM images, noise can be eliminated efficiently with optimal choice of scan rate from real-time SEM images, without generating corruption or increasing scanning time.


Subject(s)
Image Processing, Computer-Assisted/methods , Microscopy, Electron, Scanning/methods , Signal-To-Noise Ratio , Animals , Culicidae/ultrastructure , Extremities/anatomy & histology
6.
Scanning ; 35(3): 205-12, 2013.
Article in English | MEDLINE | ID: mdl-22961698

ABSTRACT

A number of techniques have been proposed during the last three decades for noise variance and signal-to-noise ratio (SNR) estimation in digital images. While some methods have shown reliability and accuracy in SNR and noise variance estimations, other methods are dependent on the nature of the images and perform well on a limited number of image types. In this article, we prove the accuracy and the efficiency of the image noise cross-correlation estimation model, vs. other existing estimators, when applied to different types of scanning electron microscope images.

7.
Scanning ; 35(2): 75-87, 2013.
Article in English | MEDLINE | ID: mdl-22777599

ABSTRACT

Detection of cracks from stainless steel pipe images is done using contrast stretching technique. The technique is based on an image filter technique through mathematical morphology that can expose the cracks. The cracks are highlighted and noise removal is done efficiently while still retaining the edges. An automated crack detection system with a camera platform has been successfully implemented. We compare crack extraction in terms of quality measures with those of Otsu's threshold technique and the another technique (Iyer and Sinha, 2005). The algorithm shown is able to achieve good results and perform better than these other techniques.

8.
J Microsc ; 248(2): 120-8, 2012 Nov.
Article in English | MEDLINE | ID: mdl-22900970

ABSTRACT

A new technique for estimation of signal-to-noise ratio in scanning electron microscope images is reported. The method is based on the image noise cross-correlation estimation model recently developed. We derive the basic performance limits on a single image signal-to-noise ratio estimation using the Cramer-Rao inequality. The results are compared with those from existing estimation methods including the nearest neighbourhood (the simple method), the first order linear interpolator, and the autoregressive based estimator. The comparisons were made using several tests involving different images within the performance bounds. From the results obtained, the efficiency and accuracy of image noise cross-correlation estimation technique is considerably better than the other three methods.

9.
Scanning ; 33(2): 82-93, 2011.
Article in English | MEDLINE | ID: mdl-21381045

ABSTRACT

A new and robust parameter estimation technique, named image noise cross-correlation, is proposed to predict the signal-to-noise ratio (SNR) of scanning electron microscope images. The results of SNR and variance estimation values are tested and compared with nearest neighborhood and first-order interpolation. Overall, the proposed method is best as its estimations for the noise-free peak and SNR are most consistent and accurate to within a certain acceptable degree, compared with the others.

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