Neurotropin effect on the deformability and electrophoretic mobility of rabbit erythrocytes.

The effect of neurotropin (a drug with antioxidant action) on the deformability and electrophoretic mobility (EM) of rabbit erythrocytes was investigated and compared to that of endotoxin from E. Coli (0111:B4) using an electrooptical technique. Endotoxin decreased the erythrocyte deformability in vitro. Upon incubation with neurotropin the endotoxin effect was reversed, i.e. the deformability of the erythrocytes increased. The electrophoretic mobility of red blood cells exposed to the simultaneous action of endotoxin and neurotropin was substantially closer to that of the control cells, as compared to the one of erythrocytes incubated with endotoxin alone. Neurotropin (1 mg.kg-1 given 1 hour after endotoxin administration) decreased the mortality of the animals, injected with endotoxin (2 mg.kg-1) namely by in the first day 63.6% versus 9.1%.

A method of exocrine pancreatectomy in the rat.

The abdominal cavity of male albino rats, average weight 250 g, was opened and the duodenum approached where the ductus choledochus joins. Pancreatic ducts empty into the lower third of the choledochus. The ductus choledochus was cannulated with a Teflon catheter beneath the liver and its other end was brought out at the neck of the rat. Tissue glue was introduced into the lower part of the choledochus towards the liver. Another Teflon catheter was introduced into the duodenum, its second end brought out at the neck and connected with the first catheter by means of a metal tube, thus preserving bile flow from the liver to the duodenum. Tissue glue injection results in complete atrophy of the exocrine pancreas within 2-15 days. The islets of Langerhans remained intact.