Gamma Radiation-Mediated Synthesis of Antimicrobial Polyurethane Foam/Silver Nanoparticles.

Nosocomial infections represent a major threat within healthcare systems worldwide, underscoring the critical need for materials with antimicrobial properties. This study presents the development of polyurethane foam embedded with silver nanoparticles (PUF/AgNPs) using a rapid, eco-friendly, in situ radiochemical synthesis method. The nanocomposites were characterized by UV-vis and FTIR spectroscopy, scanning electron microscopy coupled with energy dispersive X-ray technique (SEM/EDX), differential scanning calorimetry (DSC), dynamic mechanical analysis (DMA), tensile and compression strengths, antimicrobial activity, and foam toxicity tests. The resulting PUF/AgNPs demonstrated prolonged stability (over 12 months) and good dispersion of AgNPs. Also, the samples presented higher levels of hardness compared to samples without AgNPs (deformation of 1682 µm for V1 vs. 4307 µm for V0, under a 5 N force), tensile and compression strength of 1.80 MPa and 0.34 Mpa, respectively. Importantly, they exhibited potent antimicrobial activity against a broad range of bacteria (including Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, and Enterococcus faecalis) and a fungal mixture (no fungal growth on the sample surface was observed after 28 days of exposure). Furthermore, these materials were non-toxic to human keratinocytes, which kept their specific morphology after 24 h of incubation, highlighting their potential for safe use in biomedical applications. We envision promising applications for PUF/AgNPs in hospital bed mattresses and antimicrobial mats, offering a practical strategy to reduce nosocomial infections and enhance patient safety within healthcare facilities.

The Contribution of BaTiO3 to the Stability Improvement of Ethylene-Propylene-Diene Rubber: Part I-Pristine Filler.

This study presents the functional effects of BaTiO3 powder loaded in ethylene-propylene-diene rubber (EPDM) in three concentrations: 0, 1, and 2.5 phr. The characterization of mechanical properties, oxidation strength, and biological vulnerability is achieved on these materials subjected to an accelerated degradation stimulated by their γ-irradiation at 50 and 100 kGy. The thermal performances of these materials are improved when the content of filler becomes higher. The results obtained by chemiluminescence, FTIR-ATR, and mechanical testing indicate that the loading of 2.5 phr is the most proper composition that resists for a long time after it is γ-irradiated at a high dose. If the oxidation starts at 176 °C in the pristine polymer, it becomes significant at 188 and 210 °C in the case of composites containing 1 and 2.5 phr of filler, respectively. The radiation treatment induces a significant stability improvement measured by the enlargement of temperature range by more than 1.5 times, which explains the durability growth for the radiation-processed studied composites. The extension of the stability period is also based on the interaction between degrading polymer substrate and particle surface in the composite richest in titanate fraction when the exposure is 100 kGy was analyzed. The mechanical testing as well as the FTIR investigation clearly delimits the positive effects of carbon black on the functionality of EPDM/BaTiO3 composites. The contribution of carbon black is a defining feature of the studied composites based on the nucleation of the host matrix by which the polymer properties are effectively ameliorated.

Bioremediation of Wastewater Using Yeast Strains: An Assessment of Contaminant Removal Efficiency.

The main goal of wastewater treatment is to significantly reduce organic compounds, micronutrients (nitrogen and phosphorus) and heavy metals and other contaminants (pathogens, pharmaceuticals and industrial chemicals). In this work, the efficiency of removing different contaminants (COD, NO3-, NO2-, NH4+, PO43-, SO42-, Pb2+, Cd2+) from synthetic wastewater was tested using five different yeast strains: Kluyveromyces marxianus CMGBP16 (P1), Saccharomyces cerevisiae S228C (P2), Saccharomyces cerevisiae CM6B70 (P3), Saccharomyces cerevisiae CMGB234 (P4) and Pichia anomala CMGB88 (P5). The results showed a removal efficiency of up to 70% of COD, 97% of nitrate, 80% of nitrite, 93% of phosphate and 70% of sulfate ions for synthetic wastewater contaminated with Pb2+ (43 mg/L) and Cd2+ ions (39 mg/L). In contrast, the results showed an increase in ammonium ions, especially in the presence of Pb2+ ions. The yeast strains showed a high capacity to reduce Pb2+ (up to 96%) and Cd2+ (up to 40%) ions compared to the initial concentrations. In presence of a crude biosurfactant, the removal efficiency increased up to 99% for Pb2+ and 56% for Cd2+ simultaneously with an increase in yeast biomass of up to 11 times. The results, which were obtained in the absence of aeration and in neutral pH conditions, proved a high potential for practical applications in the biotreatment of the wastewater and the recovery of Pb and Cd ions, with a high benefit-cost ratio.

Structural Insights into LDPE/UHMWPE Blends Processed by γ-Irradiation.

Ultra-high-molecular-weight polyethylene (UHMWPE) matrices containing low-density polyethylene (LDPE), hydroxyapatite (HAp) as filler, and rosemary extract (RM) as stabilizer were investigated for their qualification for long-term applications. The significant contributions of the blend components were analyzed, and variations in mechanical properties, oxidation strength, thermal behavior, crystallinity, and wettability were discussed. SEM images of microstructural peculiarities completed the introspective survey. The stability improvement due to the presence of both additives was an increase in the total degradation period of 67% in comparison with an unmodified HDPE/UHMWPE blend when the materials were subjected to a 50 kGy γ-dose. There was growth in activation energies from 121 kJ mol-1 to 139 kJ mol-1 when HAp and rosemary extract delayed oxidation. The exposure of samples to the action of γ-rays was found to be a proper procedure for accomplishing accelerated oxidative degradation. The presence of rosemary extract and HAp powder significantly increased the thermal and oxidation resistances. The calculation of material lifetimes at various temperatures provided meaningful information on the wearability and integrity of the inspected composites.

Nutrient and organic pollutants removal in synthetic wastewater by Pseudomonas aeruginosa and Chryseobacterium sp./biofilter systems.

Nutrient and organic pollution raise serious problems for aquatic ecosystems through the accumulation of organic carbon, the reduction of light penetration, and the loss of submerged aquatic vegetation. The over-enrichment of water with nitrogen and phosphorus leads to an imbalance in nutrient ratios, creating favorable conditions for toxic algal blooms, formation of oxygen-depleted water, etc. Thus, developing new technological solutions to reduce their amount is imperative. The present study investigates the capacity of Pseudomonas aeruginosa and Chryseobacterium sp. bacterial strains to form biofilm on solid support (biofilter), both individually and in tandem, using various analytical techniques. Also, the biofilm/biofilter systems' efficiency in removing nutrients such as nitrate, nitrite, ammonium, and phosphate ions from municipal wastewaters is assessed. The results showed a reduction of nutrient pollution of up to 91%, 98%, 55%, and 71% for nitrite, nitrate, ammonium, and phosphate ions. A reduction of about 78% of COD was also observed. The results were obtained in the absence of an additional aeration process, thus having a great potential for reducing total costs of wastewater treatment and developing ecological systems for wastewater management.

High-Efficiency Biocidal Solution Based on Radiochemically Synthesized Cu-Au Alloy Nanoparticles.

The use of nanotechnologies in the applied biomedical sciences can offer a new way to treat infections and disinfect surfaces, materials, and products contaminated with various types of viruses, bacteria, and fungi. The Cu-Au nanoparticles (NPs) were obtained by an eco-friendly method that allowed the obtaining in a one-step process of size controlled, well dispersed, fully reduced, highly stable NPs at very mild conditions, using high energy ionizing radiations. The gamma irradiation was performed in an aqueous system of Cu2+/Au3+/Sodium Dodecyl Sulfate (SDS)/Ethylene Glycol. After irradiation, the change of color to ruby-red was the first indicator for the formation of NPs. Moreover, the UV-Vis spectra showed a maximum absorption peak between 524 and 540 nm, depending on the copper amount. The Cu-Au NPs presented nearly spherical shapes, sizes between 20 and 90 nm, and a zeta potential of about -44 mV indicating a good electrostatic stability. The biocidal properties performed according to various standards applied in the medical area, in dirty conditions, showed a 5 lg reduction for Staphylococcus aureus, Pseudomonas aeruginosa, and Enterococcus hirae, a 5 lg reduction for both enveloped and non-enveloped viruses such as Adenovirus type 5, Murine Norovirus, and human Coronavirus 229E, and a 4 lg reduction for Candida albicans, respectively. Thus, the radiochemically synthesized Cu-Au alloy NPs proved to have high biocide efficiency against the tested bacteria, fungi, and viruses (both encapsulated and non-encapsulated). Therefore, these nanoparticle solutions are suitable to be used as disinfectants in the decontamination of hospital surfaces or public areas characterized by high levels of microbiological contamination.

Fe-N Co-Doped Titanium Dioxide Nanoparticles Induce Cell Death in Human Lung Fibroblasts in a p53-Independent Manner.

The advancement of nanotechnology in the last decade has developed an abundance of novel and intriguing TiO2-based nanomaterials that are widely used in many sectors, including industry (as a food additive and colorant in cosmetics, paints, plastics, and toothpaste) and biomedicine (photoelectrochemical biosensing, implant coatings, drug delivery, and new emerging antimicrobial agents). Therefore, the increased use of engineered nanomaterials in the industry has raised serious concern about human exposure and their unexpected cytotoxic effects. Since inhalation is considered the most relevant way of absorbing nanomaterials, different cell death mechanisms induced in MRC-5 lung fibroblasts, following the exposure to functionalized TiO2 NPs, were investigated. Long-term exposure to TiO2 nanoparticles co-doped with 1% of iron and nitrogen led to the alteration of p53 protein activity and the gene expression controlled by this suppressor (NF-kB and mdm2), DNA damage, cell cycle disruptions at the G2/M and S phases, and lysosomal membrane permeabilization and the subsequent release of cathepsin B, triggering the intrinsic pathway of apoptosis in a Bax- and p53-independent manner. Our results are of major significance, contributing to the understanding of the mechanisms underlying the interaction of these nanoparticles with in vitro biological systems, and also providing useful information for the development of new photocatalytic nanoparticles that are active in the visible spectrum, but with increased biocompatibility.