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1.
J Virol ; 90(9): 4780-4795, 2016 May.
Article in English | MEDLINE | ID: mdl-26937036

ABSTRACT

UNLABELLED: Japanese encephalitis virus (JEV) can invade the central nervous system and consequently induce neuroinflammation, which is characterized by profound neuronal cell damage accompanied by astrogliosis and microgliosis. Albeit microRNAs (miRNAs) have emerged as major regulatory noncoding RNAs with profound effects on inflammatory response, it is unknown how astrocytic miRNAs regulate JEV-induced inflammation. Here, we found the involvement of miR-19b-3p in regulating the JEV-induced inflammatory responsein vitroandin vivo The data demonstrated that miR-19b-3p is upregulated in cultured cells and mouse brain tissues during JEV infection. Overexpression of miR-19b-3p led to increased production of inflammatory cytokines, including tumor necrosis factor alpha, interleukin-6, interleukin-1ß, and chemokine (C-C motif) ligand 5, after JEV infection, whereas knockdown of miR-19b-3p had completely opposite effects. Mechanistically, miR-19b-3p modulated the JEV-induced inflammatory response via targeting ring finger protein 11, a negative regulator of nuclear factor kappa B signaling. We also found that inhibition of ring finger protein 11 by miR-19b-3p resulted in accumulation of nuclear factor kappa B in the nucleus, which in turn led to higher production of inflammatory cytokines.In vivosilencing of miR-19b-3p by a specific antagomir reinvigorates the expression level of RNF11, which in turn reduces the production of inflammatory cytokines, abrogates gliosis and neuronal cell death, and eventually improves the survival rate in the mouse model. Collectively, our results demonstrate that miR-19b-3p positively regulates the JEV-induced inflammatory response. Thus, miR-19b-3p targeting may constitute a thought-provoking approach to rein in JEV-induced inflammation. IMPORTANCE: Japanese encephalitis virus (JEV) is one of the major causes of acute encephalitis in humans worldwide. The pathological features of JEV-induced encephalitis are inflammatory reactions and neurological diseases resulting from glia activation. MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression posttranscriptionally. Accumulating data indicate that miRNAs regulate a variety of cellular processes, including the host inflammatory response under pathological conditions. Recently, a few studies demonstrated the role of miRNAs in a JEV-induced inflammatory response in microglia; however, their role in an astrocyte-derived inflammatory response is largely unknown. The present study reveals that miR-19b-3p targets ring finger protein 11 in glia and promotes inflammatory cytokine production by enhancing nuclear factor kappa B activity in these cells. Moreover, administration of an miR-19b-3p-specific antagomir in JEV-infected mice reduces neuroinflammation and lethality. These findings suggest a new insight into the molecular mechanism of the JEV-induced inflammatory response and provide a possible therapeutic entry point for treating viral encephalitis.


Subject(s)
Carrier Proteins/genetics , Encephalitis Virus, Japanese/physiology , Encephalitis, Japanese/genetics , Encephalitis, Japanese/virology , MicroRNAs/genetics , RNA Interference , Animals , Astrocytes/metabolism , Astrocytes/virology , Base Sequence , Binding Sites , Carrier Proteins/chemistry , Cytokines/genetics , Cytokines/metabolism , DNA-Binding Proteins , Disease Models, Animal , Encephalitis, Japanese/drug therapy , Encephalitis, Japanese/metabolism , Encephalitis, Japanese/mortality , Gene Expression Profiling , Gene Expression Regulation , Host-Pathogen Interactions , Humans , Inflammation Mediators/metabolism , Intracellular Signaling Peptides and Proteins , Mice , MicroRNAs/chemistry , NF-kappa B , Oligonucleotides/genetics , RNA, Messenger/chemistry , RNA, Messenger/genetics , Signal Transduction
2.
J Immunol ; 195(5): 2251-62, 2015 Sep 01.
Article in English | MEDLINE | ID: mdl-26202983

ABSTRACT

Japanese encephalitis virus (JEV) can target CNS and cause neuroinflammation that is characterized by profound neuronal damage and concomitant microgliosis/astrogliosis. Although microRNAs (miRNAs) have emerged as a major regulatory network with profound effects on inflammatory response, it is less clear how they regulate JEV-induced inflammation. In this study, we found that miR-15b is involved in modulating the JEV-induced inflammatory response. The data demonstrate that miR-15b is upregulated during JEV infection of glial cells and mouse brains. In vitro overexpression of miR-15b enhances the JEV-induced inflammatory response, whereas inhibition of miR-15b decreases it. Mechanistically, ring finger protein 125 (RNF125), a negative regulator of RIG-I signaling, is identified as a direct target of miR-15b in the context of JEV infection. Furthermore, inhibition of RNF125 by miR-15b results in an elevation in RIG-I levels, which, in turn, leads to a higher production of proinflammatory cytokines and type I IFN. In vivo knockdown of virus-induced miR-15b by antagomir-15b restores the expression of RNF125, reduces the production of inflammatory cytokines, attenuates glial activation and neuronal damage, decreases viral burden in the brain, and improves survival in the mouse model. Taken together, our results indicate that miR-15b modulates the inflammatory response during JEV infection by negative regulation of RNF125 expression. Therefore, miR-15b targeting may constitute an interesting and promising approach to control viral-induced neuroinflammation.


Subject(s)
Encephalitis Virus, Japanese/immunology , Encephalitis, Japanese/immunology , Inflammation/immunology , MicroRNAs/immunology , Ubiquitin-Protein Ligases/immunology , 3' Untranslated Regions/genetics , 3' Untranslated Regions/immunology , Animals , Blotting, Western , Brain/immunology , Brain/metabolism , Brain/virology , Cell Line , Cell Line, Tumor , Cytokines/immunology , Cytokines/metabolism , Encephalitis Virus, Japanese/physiology , Encephalitis, Japanese/genetics , Encephalitis, Japanese/virology , Gene Expression Regulation/immunology , HeLa Cells , Host-Pathogen Interactions/immunology , Humans , Inflammation/genetics , Inflammation/virology , Inflammation Mediators/immunology , Inflammation Mediators/metabolism , Mice, Inbred BALB C , MicroRNAs/genetics , RNA Interference , Reverse Transcriptase Polymerase Chain Reaction , Ubiquitin-Protein Ligases/genetics , Ubiquitin-Protein Ligases/metabolism
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