ABSTRACT
The large number of nanomaterial-based applications emerging in the materials and life sciences and the foreseeable increasing use of these materials require methods that evaluate and characterize the toxic potential of these nanomaterials to keep safety risks to people and environment as low as possible. As nanomaterial toxicity is influenced by a variety of parameters like size, shape, chemical composition, and surface chemistry, high throughput screening (HTS) platforms are recommended for assessing cytotoxicity. Such platforms are not yet available for genotoxicity testing. Here, we present first results obtained for application-relevant nanomaterials using an automatable genotoxicity platform that relies on the quantification of the phosphorylated histone H2AX (γ-H2AX) for detecting DNA double strand breaks (DSBs) and the automated microscope system AKLIDES® for measuring integral fluorescence intensities at different excitation wavelengths. This platform is used to test the genotoxic potential of 30 nm-sized citrate-stabilized gold nanoparticles (Au-NPs) as well as micellar encapsulated iron oxide nanoparticles (FeOx-NPs) and different cadmium (Cd)-based semiconductor quantum dots (QDs), thereby also searching for positive and negative controls as reference materials. In addition, the influence of the QD shell composition on the genotoxic potential of these Cd-based QDs was studied, using CdSe cores as well as CdSe/CdS core/shell and CdSe/CdS/ZnS core/shell/shell QDs. Our results clearly revealed the genotoxicity of the Au-NPs and its absence in the FeOx-NPs. The genotoxicity of the Cd-QDs correlates with the shielding of their Cd-containing core, with the core/shell/shell architecture preventing genotoxicity risks. The fact that none of these nanomaterials showed cytotoxicity at the chosen particle concentrations in a conventional cell viability assay underlines the importance of genotoxicity studies to assess the hazardous potential of nanomaterials.
Subject(s)
Cadmium/chemistry , Histones/metabolism , Mutagenicity Tests/methods , Nanostructures/toxicity , Quantum Dots/chemistry , Cadmium/toxicity , Cell Survival , DNA Breaks, Double-Stranded/drug effects , Ferric Compounds/chemistry , Ferric Compounds/toxicity , Fluorometry , Gold/chemistry , Gold/toxicity , Metal Nanoparticles/chemistry , Metal Nanoparticles/toxicity , Mutagenicity Tests/instrumentation , Nanostructures/chemistry , Particle Size , Phosphorylation/drug effects , Quantum Dots/toxicityABSTRACT
Recent work in smoking cessation has focused on cognitive factors related to relapse and maintenance. The present investigation utilized an established self-efficacy-based questionnaire to determine whether sex, length of smoking history, successful quitting attempts, and environmental events affected subjects' perceived confidence of resisting smoking urges. It was hypothesized that: (a) subjects with fewer years smoking would have higher self-efficacy than those smoking for longer periods; (b) reported self-efficacy would be lowest for those situations involving negative moods; and (c) successful quitters would report higher self-efficacy than unsuccessful quitters. The second and third hypotheses were strongly confirmed, and a nonsignificant tendency in the expected direction was found for the first hypothesis. The results were generally consistent with the self-efficacy and the Abstinence Violation Effect (AVE) models.
