Factors controlling accumulation of organic carbon in a rift-lake, Oligocene Vietnam.

Understanding of the processes of petroleum source rock (SR) accumulation in lacustrine rift basins and the behavior of lake systems as long-term carbon sinks is fragmentary. Investigation of an 800 m thick (500 m core and ~ 300 m outcrop), deep-lacustrine, Oligocene section in Vietnam, provides a rare insight into the controls and deposition of organic carbon (OC) and SR formation in continental rift basins. A multidisciplinary dataset, combining elemental data, inorganic and organic geochemistry with sedimentology, shows that the richest alginite-prone, sapropelic SR developed during periods of relative tectonic quiescence characterized by moderate primary productivity in a mainly dysoxic lacustrine basin. Increased rift activity and further development of graben morphology intensified water column stratification and anoxia, which hindered nutrient recycling. Sapropelic organic matter (OM) continued to accumulate, but with increasing amorphous OM content and decreasing total OC values. Periods of increased seasonality were characterized by thermocline weakening, enhanced mixing of water columns, increased primary productivity and diatom blooming. The results suggest that a change from dysoxia towards anoxia or extreme primary productivity does not necessarily enhance OC burial and SR quality. External nutrient input from a phosphate-rich hinterland is sufficient for sapropel formation, whereas the main limiting factor is methanogenesis.

Danish guidelines on management of otitis media in preschool children.

INTRODUCTION: Otitis media is one of the most common diseases in small children. This underlines the importance of optimizing diagnostics and treatment of the condition. Recent literature points toward a stricter approach to diagnosing acute otitis media (AOM). Moreover, ventilating tube treatment for recurrent AOM (RAOM) and chronic otitis media with effusion (COME) has become the most frequently performed surgical procedure in pre-school children. Therefore, the Danish Health and Medicines Authority and the Danish Society of Otorhinolaryngology, Head and Neck Surgery deemed it necessary to update the Danish guidelines regarding the diagnostic criteria for acute otitis media and surgical treatment of RAOM and COME. METHODS: The GRADE system (The Grading of Recommendations Assessment, Development and Evaluation) was used in order to comply with current standards of evidence assessment in formulation of recommendations. An extensive literature search was conducted between July and December 2014. The quality of the existing literature was assessed using AGREE II (Appraisal of Guidelines for Research & Evaluation), AMSTAR (assessing the Methodological Quality of Systematic Reviews), QUADAS-2 (Quality of Diagnostic Accuracy Studies), Cochrane Risk of Bias Tool for randomized trials and ACROBAT-NRSI (A Cochrane Risk of Bias Assessment Tool for Non-Randomized Studies). The working group consisted of otolaryngologists, general practitioners, pediatricians, microbiologists and epidemiologists. CONCLUSION: Recommendations for AOM diagnosis, surgical management for RAOM and COME, including the role of adenoidectomy and treatment of ventilating tube otorrhea, are proposed in the guideline.

A randomised crossover comparison of manikin ventilation through Soft Seal®, i-gel™ and AuraOnce™ supraglottic airway devices by surf lifeguards.

Forty surf lifeguards attempted to ventilate a manikin through one out of three supraglottic airways inserted in random order: the Portex® Soft Seal®; the Intersurgical® i-gel™; and the Ambu® AuraOnce™. We recorded the time to ventilate and the proportion of inflations that were successful, without and then with concurrent chest compressions. The mean (SD) time to ventilate with the Soft Seal, i-gel and AuraOnce was 35.2 (7.2)s, 15.6 (3.3)s and 35.1 (8.5) s, respectively, p < 0.0001. Concurrent chest compression prolonged the time to ventilate by 5.0 (1.3-8.1)%, p = 0.0072. The rate of successful ventilations through the Soft Seal (100%) was more than through the AuraOnce (92%), p < 0.0001, neither of which was different from the i-gel (97%). The mean (SD) tidal volumes through the Soft Seal, i-gel and AuraOnce were 0.65 (0.14) l, 0.50 (0.16) l and 0.39 (0.19) l, respectively. Most lifeguards (85%) preferred the i-gel. Ventilation through supraglottic airway devices may be considered for resuscitation by surf lifeguards.

Enzyme replacement therapy for alpha-mannosidosis: 12 months follow-up of a single centre, randomised, multiple dose study.

BACKGROUND: Alpha-mannosidosis (OMIM 248500) is a rare lysosomal storage disease (LSD) caused by alpha-mannosidase deficiency. Manifestations include intellectual disabilities, facial characteristics and hearing impairment. A recombinant human alpha-mannosidase (rhLAMAN) has been developed for weekly intravenous enzyme replacement therapy (ERT). We present the preliminary data after 12 months of treatment. METHODS: This is a phase I-II study to evaluate safety and efficacy of rhLAMAN. Ten patients (7-17 y) were treated. We investigated efficacy by testing motor function (6-minutes-Walk-Test (6-MWT), 3-min-Stair-Climb-Test (3-MSCT), The Bruininks-Oseretsky Test of Motor Proficiency (BOT2), cognitive function (Leiter-R), oligosaccharides in serum, urine and CSF and Tau- and GFA-protein in CSF. RESULTS: Oligosaccharides: S-, U- and CSF-oligosaccharides decreased 88.6% (CI -92.0 -85.2, p < 0.001), 54.1% (CI -69.5- -38.7, p < 0,001), and 25.7% (CI -44.3- -7.1, p < 0.05), respectively. Biomarkers: CSF-Tau- and GFA-protein decreased 15%, p < 0.009) and 32.5, p < 0.001 respectively. Motor function: Improvements in 3MSCT (31 steps (CI 6.8-40.5, p < 0.01) and in 6MWT (60.4 m (CI -8.9 -51.1, NS) were achieved. Cognitive function: Improvement in the total Equivalence Age of 4 months (0.34) was achieved in the Leiter R test (CI -0.2-0.8, NS). CONCLUSIONS: These data suggest that rhLAMAN may be an encouraging new treatment for patients with alpha-mannosidosis.The study is designed to continue for a total of 18 months. Longer-term follow-up of patients in this study and the future placebo-controlled phase 3 trial are needed to provide greater support for the findings in this study.

Elimination of mange mites Sarcoptes scabiei var. suis from two naturally infested Danish sow herds using a single injection regime with doramectin.

Attempts to eliminate Sarcoptes scabiei var. suis were made in 2 naturally infested sow herds, by intramuscular (i.m.) injection of doramectin (Dectomax, Pfizer, New York, USA). A single injection strategy was used. In one of the herds, the environment was treated with an acaricide following dry cleaning of floors, walls and equipment. In the second herd, no environmental treatment was performed. Results were measured by skin lesion scoring, ear scrapings to show Sarcoptes scabiei var. suis, and calculating rubbing index throughout the observation period of 20 months following treatment. Skin lesion scores decreased and stayed low following treatment for the entire observation period. Live Sarcoptes scabiei var. suis mites were isolated prior to treatment from both herds, but not following treatment. Rubbing index decreased following treatment, but was occasionally at or above 0.4. The results of these studies indicate that elimination of Sarcoptes scabiei var. suis from 2 naturally infested herds was successful, using doramectin in a single injection strategy. Precautions must be taken to ensure adequate dosing of every pig, and to avoid reinfestation due to poor biosecurity.

Urinary albumin excretion and 24-hour blood pressure as predictors of pre-eclampsia in Type I diabetes.

AIMS/HYPOTHESIS: To evaluate the value of 24-h blood pressure monitoring compared to office blood pressure and urinary albumin excretion in predicting pre-eclampsia in Type I (insulin-dependent) diabetes mellitus. METHODS: The study included 136 consecutive pregnancies in Caucasian women with Type I diabetes before gestation without diabetic nephropathy, anamnestic hypertension or early abortion. Values of urinary albumin excretion and office blood pressure before pregnancy and the HbA1C value at the time of conception were obtained. Microalbuminuria was defined as urinary albumin excretion of 30-300 mg/24 h in two out of three consecutive urine samples. Single measurements of 24-h urinary albumin excretion, office blood pressure and HbA1C were done five 5 times during pregnancy. In a subgroup of 74 women 24-h blood pressure measurements were done at 10 and 28 weeks of gestation. Pre-eclampsia was defined as office blood pressure higher than 140/90 mmHg accompanied by proteinuria above 0.3 g/24 h later than 20 weeks of gestation. RESULTS: Urinary albumin excretion and systolic blood pressure were higher before and throughout pregnancy in 14 women developing pre-eclampsia compared with women remaining normotensive (p <0.001; p < 0.05, respectively). By logistic regression analysis the best predictor for pre-eclampsia was microalbuminuria before pregnancy (p < 0.05) with no additive predictive effect of 24-h blood pressure or office blood pressure measurement. The night:day ratio of blood pressure was similar in the two groups. CONCLUSION/INTERPRETATION: Microalbuminuria before pregnancy is the strongest predictor of pre-eclampsia in Type I diabetes. Measuring 24-h blood pressure early in pregnancy did not improve the ability to identify women at risk.

Gait disturbance interpreted as cerebellar ataxia after MMR vaccination at 15 months of age: a follow-up study.

Measles, mumps and rubella (MMR) vaccination was included in the Danish childhood vaccination programme in 1987. During the following 10-y period, 550 notification records of adverse events after MMR vaccination at 15 mo of age have been registered, and a total of 41 notifications have included "gait disturbance". This corresponds to a frequency of 8 per 100,000 doses of MMR vaccine used for 15-mo-old children. The symptoms and signs are characteristic of cerebellar ataxia. In 28 notifications, the descriptions by the doctors included only "gait disturbance", while in 13 an additional interpretation was included. Thirty-two parents (78%) filled in a questionnaire and 26 (63%) agreed to participate in a clinical follow-up study. The gait disturbance symptoms mainly occurred 7-14 d after the vaccination, and the duration was median 1-2 wk (range 1 d to more than 4 mo). One-third of the children had symptoms lasting more than 2 wk. Significantly more children with long duration of symptoms had some kind of complaint or clinical signs at the follow-up in 1997. Gait disturbance registered after MMR vaccination seems to be more frequent than hitherto reported. Most cases are mild and short-lasting and a longer duration of symptoms seems to be predictive of late sequelae. A clinical diagnosis of cerebellar ataxia after MMR and the exact frequency of this adverse event remains to be tested in prospective studies.

Pressure transfer between intracranial and cochlear fluids in patients with Meniere's disease.

OBJECTIVES: To elucidate the pressure transfer between intracranial and labyrinthine fluids in patients with well-defined unilateral Meniere's disease. STUDY DESIGN: Eleven patients previously exposed to hypobaric pressure agreed to be investigated further with the tympanic membrane displacement (TMD) technique. TMD was used to indirectly analyze perilymph pressure changes as the result of changes in body position. METHODS: Repeated measurements for both the diseased and the healthy ears were made with the patients supine and then in a sitting position. The TMD parameters for the maximum inward displacement, the Vi, and the mean volume displacement, the Vm, were calculated and compared. RESULTS: The paired comparison showed statistically significant larger Vi values for both ears in the supine position. A similar tendency was observed for the Vm value. This difference of the Vi was significantly larger for the diseased ear compared with the currently healthy ear. The results were compared to the audiometric and electrocochleographic results previously obtained on the same patients when they were subjected to hypobaric pressure. Patients who experienced the largest differences in hearing level thresholds in the lower frequencies also showed the greatest differences in TMD values as the result of postural changes. CONCLUSIONS: Despite the limited number, the statistically supported results suggest a relation between the efficiency of the routes of pressure transfer and the observed effect of hypobaric exposure. The results also indicate that for the patients tested, the routes of communication are more effective in the diseased ear than in the healthy ear--a condition that may relate to the pathogeneses of Meniere's disease.

The relationship between the acoustic reflex threshold and levels of loudness categories in hearing-impaired listeners.

When applied as a tool for hearing aid fitting, categorical loudness scaling (CLS) is time consuming and not feasible in all subjects. It is therefore desirable to use objective measures for accurate prediction of loudness categories among hearing-impaired individuals. The present study aimed at exploring whether loudness perception at the ART is constant with varying hearing threshold. Seventy-five subjects with various degrees of hearing impairment, measurable acoustic reflex and normal middle ear function participated. The HTLs, ARTs and the levels of six loudness categories at frequencies 0.5, 1, 2 and 4 kHz were determined for all subjects. Loudness at the ART was found to be correlated with the amount of hearing loss. On the basis of these results, it is concluded that the ART cannot be used for accurate estimation of loudness in hearing-impaired subjects.

The acoustic reflex threshold: not predictive for loudness perception in normally-hearing listeners.

The working hypothesis of an ongoing study is that the quick and reliable procedure of acoustic reflex threshold (ART) determination in conjunction with measurements of HTL may yield accurate estimates of loudness. The aim of this study was to investigate whether differences in loudness in normally-hearing subjects are reflected in the ARTs and to collect normal material with respect to pure-tone elicited ART and loudness categories. Categorical loudness scaling (CLS) and ART measurements were performed at frequencies of 0.5, 1, 2 and 4 kHz in 60 normally-hearing subjects (HTL<20 dB HL, 26 males, 34 females, aged 21-63 years) with no history or sequelae of middle ear disease. Subjects reporting disturbing tinnitus were excluded. The results show that the ART is not a predictor of individual loudness perception for normally-hearing subjects. Using a numerical scale (HTL=0, 'very soft'=5, 'soft'=15, 'OK'=25, 'loud'=35, 'very loud'=45 and 'too loud'=50) loudness for pure tones grows almost linearly at approximately 0.4 arbitrary loudness units per dB below the 'loud' category. Above the 'loud' category the slope is around 1 unit per dB. The median ART was 85 dB HL at frequencies of 0.5, 1, 2 and 4 kHz. No differences in loudness perception across frequencies were found.

Loudness perception is influenced by long-term hearing aid use.

The aim of this study was to explore possible differences in the perception of loudness between long-term hearing aid full-time users and non-users. Categorical loudness scaling using pure-tone stimuli was carried out by hearing-impaired subjects. The mean levels of loudness categories at one frequency (hearing threshold: 50-75 dB HL) in a group of 18 hearing aid users (daily use < or = 15 hours per day) were compared with the corresponding levels found in 18 hearing-impaired non-users with the same distribution of hearing thresholds. The results show that, for hearing losses of 50-75 dB HL, the mean level rated as 'loud' by long-term full-time users of hearing aids is 4.5 dB above the mean level of the corresponding category rated by non-users. This difference is statistically significant (P<0.05). No significant differences were found for the lower categories. Among those subjects who had been wearing hearing aids for at least six months, no significant correlation was found between the levels of the 'loud' category and the length of time that hearing aids had been used.

Long-term test-retest reliability of category loudness scaling in normal-hearing subjects using pure-tone stimuli.

The present study investigates the test-retest reliability of category loudness scaling with pure tones for each of the scaling categories: 'very soft', 'soft', 'OK', 'loud', 'very loud' and 'too loud' at the audiometric frequencies 0.5, 1 kHz, 2 kHz and 4 kHz. Category loudness scaling at two sessions separated by between 1 and 4 weeks was obtained from 16 normal-hearing subjects who all had normal otoscopy, present acoustic reflexes at audiometric frequencies 0.5-4 kHz and middle ear pressure within +/-50 daPa. Intra-subject between-session reliability was found not to be frequency dependent, and comparison with other studies revealed that reliability is not dependent on the applied stimulus signal. Test-retest reliability varied between the different categories: In the categories 'very soft', 'loud', 'very loud' and 'too loud' the test reliability is in the same range as found for hearing thresholds determination, whereas for the 'soft' and 'OK' categories it is poorer. The greater uncertainty for intermediate levels should be considered when using category loudness scaling, e.g. for calculating hearing aid parameters.

[TSH-receptor blocking antibodies in juvenile thyrotoxicosis]. / TSH-receptorblokerende antistoffer ved juvenil tyreotoksikose.

The case presented is a 12 year-old girl with hyperthyroidism due to juvenile Grave's disease, who during the course of treatment developed hypothyroidism due to TSH-receptor blocking antibodies, measured by bioassay. The bioassay used was performed on CHO-R cells measuring c-AMP. The role of radioreceptor assays and bioassays in measuring TSH receptor antibodies in hyper- and hypothyroid Graves' disease in discussed.

The influence of spontaneous otoacoustic emissions on the amplitude of transient-evoked emissions.

The aim of the study was to analyse whether the presence of spontaneous otoacoustic emissions (SOAEs) in a group of normal-hearing adults is related to larger transient evoked otoacoustic emission (TEOAE) amplitudes when compared to a similar adult population without SOAEs. Twenty-four normal-hearing subjects participated in the investigation. They were selected to form two groups of 12, one containing only subjects with measurable spontaneous emissions, the other of members who had no measurable spontaneous emissions. Each group comprised 7 males and 5 females. TEOAEs were recorded in both linear and non-linear mode, and equivalent sound pressure levels in different octave frequency bands were calculated. For each frequency band, the comparison of the equivalent sound pressure levels in the two selected groups showed statistically significant differences. Therefore, when normative TEOAE data were collected with the purpose of establishing a normative reference for the assessment of cochlear hearing loss, it is mandatory to search for and, when pertinent, to compensate for the presence of SOAEs.

[Agenesis of the corpus callosum]. / Corpus callosum-agenesi.

This article presents ten children with agenesis of the corpus callosum diagnosed during the period 1978-1992. The children were re-examined in 1992, and the results of the examination compared with the results of other authors. Among the ten children, five had other cerebral anomalies, six had eye anomalies, six were mentally retarded and two suffered from epilepsy. There was one boy with inversion on chromosome no. 1, one girl with Aicardie's syndrome, two sisters with agenesis of the corpus callosum (ACC), one of whom was suffering from diabetes insipidus, and two brothers with Walker Warburg's syndrome. ACC is often associated with mental retardation, epilepsy and eye anomalies and may be found associated to many different syndromes.

Calibration of ipsilateral stimulus transducer for acoustic reflex measurements.

Pure-tone Reference Equivalent Threshold Sound Pressure Level (RETSPL) of the ipsilateral stimulus receiver for acoustic reflex measurements on Madsen Electronics type Zodiac 901 impedance audiometer is provided. The results, obtained from 20 normal-hearing subjects, are achieved by comparing hearing threshold levels measured using a TDH 39 telephone (calibrated to ISO 389) with thresholds recorded using the ipsilateral stimulus insert phone. The calibration is referenced to an IEC-711 ear simulator and comprises the following frequencies: 125, 250, 500, 750, 1000, 1500, 2000, 3000, 4000, 6000, 8000 Hz.

[Invasive blood pressure measurements. Factual safety]. / Invasiv blodtryksmåling. Faktisk sikkerhed.

Intra-arterial blood pressure measurement is often used in patients with unstable haemodynamics. The demand for accuracy in such measurements is high. Usually these demands are fulfilled, but situations can occur where the dynamic characteristics of the system are exceeded. In order to acknowledge this situation, one must be aware of these dynamic characteristics. The significance of the system's resonance frequency and damping is described. A method to control the usability of the system is described.

[Disposable transducers for invasive blood pressure measurement. A test of the dynamic characteristics]. / Engangstransducere til invasiv blodtryksmåling. En test af de dynamiske egenskaber.

The accuracy of intra-arterial blood pressure measurement is dependent on the dynamic characteristics of the transducer. Specifications made by the manufacturers are often misleading. Four different transducers are tested in a clinically relevant setting. The resonance frequencies of all the transducers were found to be acceptable.

[Keloid of the external ear]. / Keloid på ydre øre.

Keloid formation is an unpredictable complication to normal scar tissue development. The etiology is unknown, although an individual predisposition may be present. There is a regional propensity for the head, neck and upper torso. The treatment is difficult, and this should be taken into consideration when cosmetic procedures are being contemplated. This type of surgery should be avoided in individuals with previous keloid formation. Four cases of keloid formation, two after ear lobe piercing and two after otoplasty are presented and a satisfying outcome of surgical revision with ensuing steroid medication is reported.

Growth of distortion product otoacoustic emissions with primary-tone level in humans.

This study addressed the hypotheses that the growth of the level of distortion product otoacoustic emissions (LDP) with primary-tone level reflects the behavior of a third-order nonlinear polynomial system, and that two sources exist for these distortion products. The results indicated that the 2f1-f2 otoacoustic emission in humans can be measured over a much larger stimulus range than reported previously, even for stimuli (L1 = L2) as low as 10 dB SPL (re 20 microPa). The input/output functions are best described as a straight line with a rate of growth of about 1 dB/dB of stimulus level. For stimulus levels at which metabolically active, nonlinear cochlear processes are in operation, the system does not behave as a simple third-order nonlinear polynomial. Small plateaus and sharp discontinuities or 'notches' can occur in the functions at stimulus levels of approximately 55 dB SPL. These characteristics are consistent with the notion of two separate sources of the LDP, one at low stimulus levels, and one at high levels. An alternative explanation is that the measured otoacoustic emission does not represent only the activity at a single location along the basilar membrane but includes the effects of interactions among similar signals arising from multiple locations, or from the original source via multiple paths.