ABSTRACT
In this study are reported 17 patients with G-6-PD deficiency. These subjects have been studied with double-blind trial between placebo and Paracetamol, looking for the possible hemolysis induced from drug in these children with G-6-PD deficiency. Hemolysis had been valued by various hematological parameters (Hb, erythrocyte count, reticulocytosis, bilirubinemia, haptoglobin, hemopexin, and survival of erythrocytes marked with Cr). The results (8 cases with placebo and 9 cases with paracetamol) demonstrate that paracetamol have not induced hemolysis in these G-6-PD deficient subjects.
Subject(s)
Acetaminophen/adverse effects , Anemia, Hemolytic/chemically induced , Glucosephosphate Dehydrogenase Deficiency/complications , Adolescent , Child , Child, Preschool , Double-Blind Method , Glucosephosphate Dehydrogenase Deficiency/blood , Hematologic Tests , Humans , PlacebosSubject(s)
Abnormalities, Multiple , Orofaciodigital Syndromes , Abnormalities, Multiple/diagnostic imaging , Child , Cleft Palate/complications , Eye Diseases/complications , Female , Hip Joint , Humans , Joint Diseases/complications , Joint Diseases/diagnostic imaging , Joint Instability/complications , Myopia/complications , Orofaciodigital Syndromes/diagnostic imaging , RadiographySubject(s)
Arthritis, Juvenile/drug therapy , Auranofin/therapeutic use , Child , Female , Follow-Up Studies , Humans , MaleABSTRACT
We describe a patient with primary biliary cirrhosis associated with Graves' disease and discuss the possible autoimmune connections between the two disorders.
Subject(s)
Graves Disease/complications , Liver Cirrhosis, Biliary/complications , Female , Graves Disease/pathology , Humans , Liver Cirrhosis, Biliary/pathology , Middle AgedABSTRACT
A group of 41 patients (25 males and 16 females) with mild chronic active hepatitis was studied for a mean follow-up period of 6.5 years. All patients had liver biopsy on admission and a second biopsy during the follow-up period. All but 7 patients were treated with prednisolone. Most of the patients were asymptomatic. There was no history of alcohol abuse or chronic administration of drugs. Fourteen patients were HBsAg positive and 27 HBsAg negative. Circulating autoantibodies were absent. Thirty-three patients did not demonstrate any change in disease activity. Four developed liver cirrhosis and four were apparently cured.
Subject(s)
Hepatitis, Chronic/complications , Autoantibodies/analysis , Female , Follow-Up Studies , Hepatitis B Surface Antigens/analysis , Hepatitis, Chronic/immunology , Humans , Liver Cirrhosis/complications , Male , Middle Aged , PrognosisABSTRACT
Fibroblasts can synthetize prostaglandins (particularly PGE2) "in vitro" but it still remains unclear what role they play in the regulation of fibroblast proliferation and collagen production. We report here the effect of PGE2 and indomethacin on collagen synthesis by cultured human dermal fibroblasts. PGE2 (range: 1-300 pmoles/ml) and indomethacin (range: 0.0025-1.0 micrograms/ml) did not significantly affect fibroblast collagen production, when added for 24 hours at 37 degrees C to the cultures, in comparison to controls (fibroblasts incubated for 24 hours at 37 degrees C in medium only). Prostaglandins probably modulate collagen synthesis, as described in a previous report, by means their effect on cell proliferation. It appears they do not affect the intracellular mechanism of collagen production.
Subject(s)
Collagen/biosynthesis , Indomethacin/pharmacology , Prostaglandins E/pharmacology , Skin/metabolism , Cells, Cultured , Dinoprostone , Fibroblasts/drug effects , Fibroblasts/metabolism , Humans , Skin/cytologyABSTRACT
Primary biliary cirrhosis (PBC) is a chronic cholestatic progressive liver disease frequently associated with various immunological abnormalities. We have studied the influence of normal and PBC adherent cells (AC) on peripheral blood lymphocytes (PBL) colony growth both in liquid and soft agar culture. Co-culture experiments with different combinations of AC and PBL of PBC patients and normal donors provided evidence that PBC adherent cells function abnormally. However, this impaired function is not the sole factor involved, since the number of colonies formed by PBC lymphocytes in the presence of normal AC was twice the normal value. The possibility that this increase is correlated with the state of activation of T cells is discussed in the light of the high number of DR-positive T cells found in the PBC patients studied. Finally, none of these immunological abnormalities was related to the stage of the disease.
Subject(s)
Liver Cirrhosis, Biliary/immunology , T-Lymphocytes/immunology , Adult , Cell Adhesion , Cholestasis, Extrahepatic/immunology , Female , HLA-DR Antigens , Hepatitis, Chronic/immunology , Histocompatibility Antigens Class II/immunology , Humans , Lymphocyte Activation , Lymphocyte Cooperation , Male , Middle AgedABSTRACT
A defect of cell-mediated immunity was reported in patients with Crohn's disease. In attempting to evaluate the possible role of serum factor(s) in the pathogenesis of this defect we studied the effect of sera from patients with Crohn's disease on normal lymphocyte E rosette formation and in vitro PHA responsiveness. An impairment of E rosette formation and PHA response was detected when normal donor lymphocytes were incubated with patients' sera. We concluded that the defect of cell-mediated immunity in Crohn's disease may be due to the presence of serum inhibitory factor(s) and of nutrient serum deficiencies. This defect is not correlated with clinical parameters and the duration of the disease; it is not affected by surgical resection.
