Health Risks for Consumers of Forest Ground Cover Produce Contaminated with Heavy Metals.

BACKGROUND: The activity of heavy metal (HM) mining and processing industries causes soils contamination with HM. The metals could be transferred from contaminated soils to edible plants and fungi. This study aimed to assess the content of Cd, Pb, Hg, As, and Ni in berries and edible mushrooms collected in the forests located near Miasteczko Slaskie zinc smelter and in the Lubliniec region, in the context of consumers' health risk. METHODS: The ET-AAS method was used to determine the content of Cd, Pb, Ni, and As. Mercury concentration was determined using the CV-AFS method. RESULTS: The studies showed high levels of Cd and Pb in the examined products. A statistically significant impact of the distance from the smelter on the Cd concentration in the berries was observed. Total non-cancer health risk from the combined exposure of adults to all HM in mushrooms and berries was significant when consuming the most heavily contaminated produce. The risk to children was significant, even when consuming products with moderate levels of the metals. Ingestion of Cd by children with mushrooms was related to a high cancer risk. The uncertainty of the results was determined. CONCLUSIONS: It is recommended to take action to increase awareness among residents of the areas adjacent to the forests regarding the existing health risk and to take possible measures to reduce exposure to HM.

Exposure to culturable and total microbiota in cultural heritage conservation laboratories.

OBJECTIVES: To date, the scientific source materials usually focus on microbial contamination of the museum or library collections themselves, while the exposure of persons who professionally deal with this type of objects in cultural heritage conservation laboratories is ignored. MATERIAL AND METHODS: The study was carried out in 9 naturally ventilated conservation laboratories with no history of water damage. Viable (understood as culturable) bioaerosol stationary samples were collected in both outdoor and indoor environments using 6-stage Andersen impactor. Simultaneously, stationary and personal indoor bioaerosol measurements were carried out using both Gesamtstaubprobenahme an der Person (GSP) and Button filter samplers. These measurements were complemented by evaluation of microbial content in the dust settled on conserved works of art. All impactor, filter, and settled dust samples were quantitatively examined to obtain viable and total concentrations of bacteria and fungi. All isolated microbial strains were taxonomically identified. RESULTS: At workplaces, the concentrations of viable microorganisms in air were below 2000 cfu/m3 and accounted for not more than 5.5% of total microbiota. The study showed that quantitative assessment of viable bioaerosol can be made with an Andersen impactor as well as by using Button and GSP filter samplers, irrespective of whether they are applied for personal or stationary measurements. Compared to the impactor, however, the use of filter samplers for microbial contamination monitoring substantially limits the scope of qualitative information which can be obtained. Size distribution analysis revealed that the largest "load" of microorganisms can penetrate into the respiratory tract between the trachea and terminal bronchi, and thereby may be responsible for allergic inflammations in exposed workers. CONCLUSIONS: The precise assessment of microbial hazards in conservation laboratories should comprise control of both viable and total particle counts. The hermetization of such workplaces and control of relative humidity should be implemented and maintained to assure proper hygienic conditions.

Occupational exposure to airborne microorganisms, endotoxins and ß-glucans in poultry houses at different stages of the production cycle.

The aim of the presented study was to assess the exposure of poultry workers to airborne microorganisms, endotoxins and ß-glucans during different stages of the chicken production cycle in 3 commercially-operated poultry houses. Personal and stationary sampling was carried out to assess exposure to both viable and total microbial aerosols. The stationary measurements of PM10 were performed to establish the level of endotoxins and ß-glucans. The concentrations of bacterial and fungal aerosols ranged from 2.5×10(2) CFU/m(3)-2.9×10(6) CFU/m(3), and from 1.8×10(2) CFU/m(3)-1.8×10(5) CFU/m(3), respectively. The number of culturable microorganisms was significantly lower than their total counts, constituting from 0.0004%-6.4% of the total microbial flora. The level of PM10 in poultry facilities did not exceed 4.5 mg/m(3). After the flock entered the clean house, the level of endotoxins and ß-glucans increased from below detection limit to 8,364 ng/m(3) and from 0.8 ng/m(3) to 6,886 ng/m(3), respectively. The presented study shows that professional activities in poultry farms are associated with constant exposure to bioaerosol, which may pose a health hazard to workers. It was found that workers' exposure to airborne microorganisms increased with consecutive stages of the chicken production cycle.

Bioaerosol assessment in naturally ventilated historical library building with restricted personnel access.

The aim of this study was to check the degree and identify the sources of microbial contamination of the Jasna Gora (Bright Hill) monastery library 10 years after disinfection of the incunabula collection. The registered maximum viable indoor microbial concentrations were 1,875 and 7,100 cfu/m³ for stationary and personal measurements, whereas respective total concentrations were 71,000 and 100,000 counts/m3. There was no statistically significant difference between the concentrations of viable microorganisms measured in the stationary using Andersen, GSP, and Button samplers. Moreover, GSP and Button samplers can be interchangeably applied when viable or total microbial levels are stationary or personally measured. The culturable microorganisms constituted 0.5 - 3.9% of the total microflora only. Filamentous fungi were the most prevalent outdoors, whereas Gram-positive cocci and endospore forming Gram-positive rods dominated indoors in the air and settled dust, respectively. Hence, an unrestrained infiltration of ambient air through the draughtiness of the building envelope is probably the main process responsible for indoor fungal pollution, whereas bacterial contaminants have their major sources in the indoor environment. Moreover, even a chemically cleansed library collection, having a restricted personnel access, but under the influence of ambient air, can undergo microbial contamination and becomes an important microbial emission source.

Environmental risk factors for respiratory symptoms and childhood asthma.

The presented cross-sectional study, comprised a group of 1,130 children from 13-15 years of age living in Upper Silesia, Poland, was undertaken to ascertain the role of environmental factors in the development of adverse respiratory health outcomes. To estimate the prevalence of these effects, the ISAAC questionnaire supplemented by questions related to risk factors was used. Bronchial asthma was identified in 4.5% of the children, asthma diagnosed by physicians in 8.7%, and prevalence of wheezing in the previous 12 months in 12.6%. The highest probability of wheezing was found in children with maternal genetic propensity (such as asthma, allergy), exposed to maternal smoking, or was connected with household risk factors such as the presence of dampness/mould or living in 50-year-old building. Female gender and attendance at nursery school were shown to be protective factors against wheezing. The probability of asthma was nearly twice as high in children residing in damp/mouldy dwellings, heated by coal-fired furnaces and living in the immediate vicinity of a road with heavy traffic. This study revealed that exposure to indoor (tobacco smoke, coal stove emission, mould or dampness in dwelling) and outdoor (traffic pollution) air contaminants are major environmental factors responsible for adverse respiratory health effects in children.

Viability of fungal and actinomycetal spores after microwave radiation of building materials.

The effects of microwave radiation on viability of fungal and actinomycetal spores growing on agar (medium optimal for growth) as well as on wooden panel and drywall (common building construction/finishing materials) were studied. All materials were incubated at high (97-99%) and low (32-33%) relative humidity to mimic "wet" and "dry" environmental conditions. Two microwave power densities (10 and 60 mW/cm2) and three times of exposure (5, 30, and 60 min) were tested to find the most effective parameters of radiation which could be applied to non-invasive reduction or cleaning of building materials from microbial contaminants. Additionally, a control of the surface temperature during the experiments allowed differentiation between thermal and microwave effect of such radiation. The results showed that the viability of studied microorganisms differed depending on their strains, growth conditions, power density of microwave radiation, time of exposure, and varied according to the applied combination of the two latter elements. The effect of radiation resulting in a decrease of spore viability on "wet" wooden panel and drywall was generally observed at 60 min exposure. Shorter exposure times decreased the viability of fungal spores only, while in actinomycetes colonizing the studied building materials, such radiation caused an opposite (supporting growth) effect.