ABSTRACT
The aim of this study was to evaluate the effect of the agonistic LRH analogue (D-Trp6-LRH) on LH, FSH and prolactin secretion, and its possible efficacy in female patients with anorexia nervosa. The study included 18 female patients with anorexia nervosa and 7 healthy women in the same age group. The patients with anorexia nervosa were divided into two groups of 9 women each. The first group consisted of patients in the weight loss phase of the disorder and the second of 9 women who had achieved normal body weight. Additionally, in 6 women with anorexia nervosa serum LH concentration was determined in response to native LRH before and 14 days after the treatment with D-Trp6-LRH. Serum LH, FSH, prolactin concentrations were determined before and 5, 10, 24 and 48 h after administration of D-Trp6-LRH in a dose of 5 micrograms. The serum LH response to D-Trp6-LRH in the second group did not differ from that of the control group. However, in women with anorexia nervosa in the weight loss phase, LH release in response to administration of the analogue was significantly lower. FSH release after LRH analogue administration in both groups of patients with anorexia nervosa did not differ from that in the control group. The increased LH secretion in response to native LRH after treatment indicates that D-Trp6-LRH in low doses did not inhibit pituitary responsiveness. A dose of 5 micrograms of D-Trp6-LRH was administered in a therapeutic regimen every 48 h from 1 to 3 months to 7 of 9 patients with anorexia nervosa who exhibited amenorrhoea in spite of normalization of body weight.(ABSTRACT TRUNCATED AT 250 WORDS)
Subject(s)
Anorexia Nervosa/drug therapy , Follicle Stimulating Hormone/metabolism , Gonadotropin-Releasing Hormone/analogs & derivatives , Luteinizing Hormone/metabolism , Prolactin/metabolism , Adolescent , Adult , Anorexia Nervosa/blood , Anorexia Nervosa/physiopathology , Body Weight/drug effects , Female , Follicle Stimulating Hormone/blood , Gonadotropin-Releasing Hormone/pharmacology , Humans , Luteinizing Hormone/blood , Menstruation/drug effects , Prolactin/blood , Triptorelin PamoateABSTRACT
The aim of this study was to develop a technique for the radioimmunological determination of the activity of LH-like substances in the human prostate. The material comprised 19 specimens of prostatic tissue obtained during transbladder extirpation in patients with benign prostatic hyperplasia. Tissues of human testes and human sceletal muscle were used as controls. The method adopted for LH extraction from the membrane fraction of human prostatic tissue appeared to be sufficiently specific, accurate and sensitive for routine laboratory investigations. The concentrations of the LH-like immunoreactivity in human testicular tissue was found to be 57, 46 and 70 mU per g of the membrane fraction while those of the prostatic gland tissues ranged from 34 to 155 mU per g of the membrane fraction. However such LH-like substance was not found in human sceletal muscle tissue. It seems that the LH-type activity is an indirect proof for the existence of LH receptors in the human prostate.
Subject(s)
Luteinizing Hormone/analysis , Prostate/analysis , Radioimmunoassay/methods , Aged , Humans , Male , Middle Aged , Prostatic Hyperplasia/metabolismABSTRACT
The aim of the study was to evaluate the hormonal pattern in men with idiopathic oligozoospermia and azoospermia. Material consisted of 13 sterile men, 21-34 years old (the mean - 27 years). Three of 13 patients had the third degree oligozoospermia, 3 - extreme oligozoospermia and 7 - azoospermia. The control group consisted of 11 healthy men who were found to have normozoospermia. In all patients and in control subjects the estimation of LH, FSH, prolactin (PRL), testosterone and dihydrotestosterone (DHT) in basal conditions and also PRL determination at 15, 30, 60 min after stimulation with metoclopramide (MCP 10 mg i.v.) were performed by radioimmunoassay. The results indicate that men with idiopathic oligo- or azoospermia have significantly higher prolactin level in response to dopaminergic receptor blockade with metoclopramide and significantly lower serum testosterone and DHT concentrations.
Subject(s)
Metoclopramide/therapeutic use , Oligospermia/drug therapy , Prolactin/blood , Adult , Dihydrotestosterone/blood , Follicle Stimulating Hormone/blood , Humans , Luteinizing Hormone/blood , Male , Testosterone/bloodABSTRACT
The aim of this study was to determine whether in human clinical practice destruction of the prostate gland by a malignant process is accompanied by a fall in serum testosterone and dihydrotestosterone levels. Mean serum testosterone and dihydrotestosterone levels were significantly lower and LH levels notably higher in patients with metastases than in those without metastases. No notable differences were found between serum testosterone and dihydrotestosterone levels in relation to the degree of malignancy of the tumours. Concentrations of relatively inactive androgens (DHA and delta-4-androstenedione) in the serum were normal. The fall in serum concentration levels with later increase in LH levels is probably caused by malignant destruction of the prostate gland.
Subject(s)
Hormones/blood , Prostatic Neoplasms/blood , Acid Phosphatase/blood , Aged , Dihydrotestosterone/blood , Humans , Luteinizing Hormone/blood , Male , Middle Aged , Neoplasm Staging , Prostatic Neoplasms/pathology , Testosterone/bloodSubject(s)
Adenoma/physiopathology , Adrenal Cortex Neoplasms/physiopathology , Cushing Syndrome/physiopathology , Ovary/physiopathology , Pituitary Gland, Anterior/physiopathology , Adenoma/surgery , Adolescent , Adrenal Gland Neoplasms/surgery , Adult , Cushing Syndrome/drug therapy , Cushing Syndrome/surgery , Evaluation Studies as Topic , Female , Humans , Middle AgedSubject(s)
Adipose Tissue/analysis , Milk, Human/analysis , Polychlorinated Biphenyls/analysis , Abdominal Muscles , Adolescent , Adult , Environmental Exposure , Female , Humans , Male , Poland , Pregnancy , Rural Population , Urban PopulationSubject(s)
Chromatography , Dihydrotestosterone/blood , Radioimmunoassay/methods , Testosterone/blood , Adult , Aged , Humans , Male , Middle AgedSubject(s)
Gonadotropins/blood , Hypogonadism/diagnosis , Olfaction Disorders/diagnosis , Adult , Humans , Male , SyndromeSubject(s)
Cyproterone/therapeutic use , Hirsutism/drug therapy , Adult , Female , Humans , Middle AgedABSTRACT
The effects of alpha, alpha1-dipyridyl (DP) on noradrenaline (NA), dopamine (DA) and 5-hydroxytryptamine (5-HT) levels in rat and mouse brain and on dopamine-beta-hydroxylase (DbetaH) activity in rat brain have been studied, DP decreases NA level and inhibits DbetaH activity in a dose dependent manner, without affecting DA or 5-HT levels.
Subject(s)
2,2'-Dipyridyl/pharmacology , Brain/metabolism , Dopamine beta-Hydroxylase/metabolism , Dopamine/metabolism , Norepinephrine/metabolism , Pyridines/pharmacology , Serotonin/metabolism , Animals , Brain/drug effects , Brain/enzymology , Male , Mice , Time FactorsSubject(s)
Milk, Human/analysis , Polychlorinated Biphenyls/analysis , Animals , Cattle , Female , Humans , Milk/analysis , PregnancyABSTRACT
In search for selective dopamine-beta-hydroxylase inhibitors, we studied the effect of dimethyl-, monocyclohexyl-, and dicyclohexyldithiocarbamic acids on the activity of this enzyme and on dopamine and noradrenaline levels in rat's brain. All the three derivatives inhibit the activity of dopamine-beta-hydroxylase in brain; in the tested doses, their effectiveness is similar. Moreover, all three compounds decrease noradrenaline level in brain. The tested derivatives differ from one another in their effect on dopamine level in brain. Dimethyl- and monocyclohexyl derivatives increase while dicyclohexyl derivative decreases the level of this amine.
