ABSTRACT
The possibility of using mesoporous silicon nanoparticles as amplifiers (sensitizers) of therapeutic ultrasonic exposure were studied experimentally in vitro and in vivo. The combination of nanoparticles and ultrasound led to a significant inhibition of Hep-2 cancer cell proliferation and Lewis lung carcinoma growth in mice. These results indicated good prospects of using silicon nanoparticles as sensitizers for sonodynamic therapy of tumors.
Subject(s)
Antineoplastic Agents/administration & dosage , Carcinoma, Lewis Lung/drug therapy , Silicon/administration & dosage , Animals , Antineoplastic Agents/chemistry , Cell Line, Tumor , Drug Screening Assays, Antitumor , Humans , Male , Mice, Inbred CBA , Nanoparticles/chemistry , Neoplasm Transplantation , Silicon/chemistry , Ultrasonic WavesABSTRACT
Dynamic light scattering method or laser correlation spectroscopy was applied to evaluation of the size of viruses. We measured correlation functions of the light scattered by human immunodeficiency viruses (HIV) and hepatitis A viruses (HAV) and found that size of HIV-1 (subtype A and B) and HAV virions were 104 nm and 28 nm, respectively. Comparison of these findings with electron microscopy data for fixed samples of the same viruses showed good agreement of the results.
Subject(s)
HIV-1/ultrastructure , Hepatitis A virus/ultrastructure , Cell Line , Dynamic Light Scattering , Humans , Lasers , Microscopy, Electron , Particle Size , Spectrum AnalysisABSTRACT
A model describing the process of dissociation of hydrogen bonding in water clusters when irradiated by electromagnetic field in the microwave range is suggested. The model is also applicable for the case of rupture of the covalent bond of the water molecule cluster. If the energy absorption occurs at the interface of water and polymer clusters (e.g., DNA, chitosan), degradation of the polymer chain is possible.
Subject(s)
Chitosan/chemistry , DNA/chemistry , Hydrogen Bonding/radiation effects , Microwaves , Chitosan/radiation effects , DNA/radiation effects , Electromagnetic Fields , Water/chemistryABSTRACT
Diphtheria morbidity among adult population of Moscow is growing. The tests of different modes and schemes of heterogeneous antidiphtheria serum administration have found out that the highest efficacy of serotherapy was achieved with a single intravenous dose. Another treatment of diphtheria is hemosorption. Widely used in present-day clinical practice hemosorbents provide adequate elimination of the toxin from plasma. The authors analyse the present situation in clinical symptoms, complications, lethality of diphtheria basing on the data provided by S. P. Botkin's Hospital.
Subject(s)
Diphtheria/diagnosis , Diphtheria/therapy , Adolescent , Adult , Antibodies, Bacterial/blood , Corynebacterium diphtheriae/immunology , Diphtheria/classification , Diphtheria/epidemiology , Diphtheria/immunology , Diphtheria Antitoxin/administration & dosage , Evaluation Studies as Topic , Female , Humans , Male , Middle Aged , Moscow/epidemiology , Seroepidemiologic Studies , Sorption Detoxification/methods , Time Factors , Urban Population/statistics & numerical dataSubject(s)
Carrier State/epidemiology , Diphtheria/etiology , Adult , Age Factors , Carrier State/diagnosis , Carrier State/therapy , Diagnostic Errors , Diphtheria/diagnosis , Diphtheria/epidemiology , Diphtheria/therapy , Diphtheria Antitoxin/therapeutic use , Female , Humans , Male , Middle Aged , Moscow/epidemiologySubject(s)
Botulism/physiopathology , Respiration , Adult , Female , Humans , Respiratory Insufficiency/etiologySubject(s)
Toxoplasmosis, Animal/diagnosis , Toxoplasmosis/diagnosis , Animals , Female , Humans , Pregnancy , Research , Toxoplasmosis/epidemiology , Toxoplasmosis/immunology , Toxoplasmosis, Animal/epidemiology , Toxoplasmosis, Animal/immunology , Toxoplasmosis, Congenital/diagnosis , Toxoplasmosis, Congenital/epidemiology , Toxoplasmosis, Congenital/immunology , USSRSubject(s)
Blood Transfusion , Cefazolin/therapeutic use , Erysipelas/therapy , Blood/radiation effects , Combined Modality Therapy , Female , Humans , Male , RecurrenceABSTRACT
Dependence of the enzymatic reaction rate V. catalyzed by aspartate aminotransferase, on concentration of standard human blood serum Moni-trol I ("Dade", Switzerland) was studied. In the range of moderate dilutions, which did not exceed 1.3-times, the V value altered in accordance with the blood serum concentration C. Under conditions of extensive dilutions dependence of V on blood serum concentration became complicated. The non-linear type of V dependence on blood serum concentration appears to be due to deaggregation of the enzyme molecules with formation of more active fragments as a result of blood serum dilution. Two parameters should be used for characteristics of blood serum aspartate aminotransferase activity: the enzymatic activity of undiluted blood serum as well as the ratio between specific activities of the enzyme in an associated from and the products of its dissociation, i.e. when C-1 and C-0, respectively.
Subject(s)
Aspartate Aminotransferases/blood , Humans , Kinetics , Specimen HandlingABSTRACT
The possibility of using the primary epidemiological unit, force of infection, as a criterion for evaluating the intensity of the transfer of toxoplasmosis among the population at the focus of infection has been established. The comparison of data obtained in different regions indicates that the level of the force of infection is, in general, low, but it can vary within a wide range. The problem of the suitability of using the force of infection as a criterion for dividing territories into zones with respect to toxoplasmosis is discussed.
