[Protein and DNA adsorption on dehydroxylated and aluminated silochromes]. / Adsorbtsiia belkov i DNK na degidroksilirovannykh i aliuminirovanykh silokhromakh.

A macroporous silica - silochrom - used for immobilization and purification of biopolymers was modified by dehydroxylation and alumination. The adsorption capacity of the modified silochroms for proteins and DNA is higher than that of the initial sorbent. The biopolymers adsorbed on aluminated silochrom are strongly attached to its surface.

[Removal of lipoprotein complexes of cholesterol from plasma using silochromes]. / Udalenie lipoproteidnykh kompleksov kholesterina iz plazmy krovi s ispol'zovaniem silokhromov.

Industrial silicates, silochromes, proved to be effective sorbents of cholesterol lipoprotein complexes in blood in vitro. Silochrome SH-1.5 with a diameter of pores of 180-200 nm and a capacity of 12-14 mg of cholesterol per 1 g of the sorbent exhibited the best efficiency in isolation of the complexes. At the same time, maximal unspecific binding of blood plasma proteins was observed in the systems with silochrome SH-3 with a diameter of pores of 60 nm. By use of silochrome SH-1.5 for plasmosorption the content of cholesterol was decreased from 3,600 mg/l down to 2,150 mg/l, i.e. by 40%, in blood plasma of a rabbit with experimental hypercholesterolemia.

[Urease immobilization on macroporous silicas]. / Immobilizatsiia ureazy na makroporistykh kremnezemakh.

Urease was immobilized on macroporous silicas using gamma-aminopropyl triethoxysilane and glutaraldehyde. The amount of protein on the surface, the structure of pores of the support and the purity of the initial enzyme were varied, the enzymic activity of the immobilized preparations being controlled. After the immobilization of sufficiently large quantities of the enzyme (about 3 mg protein per m2 support) about 35% of the specific activity was retained. The maximum activity per unit weight of the support was observed for silicagels and silochromes with the mean diameter of pores 70-90 nm and the specific surface area about 70 m2/g. The use of purified urease produced highly active preparations of the immobilized enzyme (17,000 U per g dry support). Freeze-drying of the immobilized enzyme in the presence of sorbitol yielded dry preparations retaining their activity.

[Nisin adsorption on silica adsorbents]. / Adsorbtsiia nizina na kremnezemnykh adsorbentakh.

It has been shown that silica adsorbents can be used for adsorption of nizin from solutions obtained after centrifugation of the fermentation broth. The optimal structure of the adsorbent pores has been determined. Silica with pores 50 to 75 nm in size provided the highest adsorption rates. The value of silica adsorption of nizin depended on the medium pH. The maximum adsorption rates were observed at pH 6.5--7. At pH 3.5 the level of nizin adsorption was low.