ABSTRACT
The authors suggest a method of obtaining purified antilymphocytic antibodies by mean of a specific immunosorbent of human or mouse lymphocytes fixated with glutharic aldehyde Such immunosorbents subjected to special treatment could be used repeatedly; their sorptive capacity was retained in such case. Only from 5 to 12% of the activity could be obtained from immunosorbents sorbed from the serum with 77--93% activity. In comparison with the initial serum, purification was from 6- to 15-fold. Thus, the suggested method provided considerable purification of the antilymphocytic preparations and permitted to obtain highly active antilymphocytic antibodies.
Subject(s)
Aldehydes/pharmacology , Antigen-Antibody Complex/pharmacology , Autoantibodies/isolation & purification , Glutaral/pharmacology , Immunosorbents/pharmacology , Lymphocytes/immunology , Lymphotoxin-alpha/isolation & purification , Animals , Cytotoxicity Tests, Immunologic , Humans , Immunosorbent Techniques , Mice , Palatine Tonsil/cytology , Thymus Gland/cytologyABSTRACT
Gamma-globulin preparations prepared by the alcoholic method from the placental and abortion sera were split during storage under the effect of proteases contained in them; fragments with a molecular weight of 50 000 and 60 000 were formed. The most intensive splitting was seen in the preparations obtained from the precipitates of B placental and abortive sera. Some delay in the gamma-globulin fragmentation could be reached by addition to protease inhibitors--epsilon-aminocapronic acid or cyclocapron.
