Metal kinetics and respiration rates in F1 generation of carabid beetles (Pterostichus oblongopunctatus F.) originating from metal-contaminated and reference areas.

We investigated resistance to metals in carabid beetles inhabiting metal-polluted and reference areas. Chronic multigeneration exposure to toxic metal concentrations may potentially result in adaptation through decreased metal uptake rate and/or increased excretion rate. The cost of resistance to pollution could be associated with increased metabolic rate. To test these predictions, laboratory cultured F(1)-generation beetles originating from metal-polluted and reference sites were exposed to food contaminated with zinc and/or cadmium for 10 weeks. After that, uncontaminated food was offered to the animals for another 3 weeks. During the experiment, internal concentrations of Cd and Zn were measured as were respiration rates of the animals. The results obtained show no significant differences in metal accumulation and excretion patterns or respiration rates between the populations. This may suggest that adaptation has not occurred in the beetles chronically exposed to toxic metal concentrations. The possible explanations for the lack of differences between the populations are discussed.

Heavy metal accumulation, heat shock protein expression and cytogenetic changes in Tetrix tenuicornis (L.) (Tetrigidae, Orthoptera) from polluted areas.

The orthopteran insect Tetrix tenuicornis, collected from polluted and unpolluted areas, was used to study heavy metal accumulation and its impact on stress protein levels and on changes in the number and morphology of chromosomes in mitotic and meiotic cells. During two consecutive years, insects were collected from polluted areas of zinc-lead mine spoils near Boleslaw (Poland) and from unpolluted areas near Busko and Staszów (Poland). T. tenuicornis from the polluted area showed 1.5, 4.03, 4.32 and 41.73 times higher concentrations of copper (Cu), zinc (Zn), lead (Pb) and cadmium (Cd), respectively, than insects of the same species collected from unpolluted areas. Insects exposed to heavy metals showed only small changes, and rather a decrease in the concentration of constitutive and inducible heat shock proteins Hsp70, the level of which increases under stress conditions. A cytogenetic study of T. tenuicornis revealed intra-population anomalies in chromosome number and morphology in mitotic and meiotic cells and the presence of an additional B chromosome in germinal cells. In 50% of females collected from polluted areas, mosaic oogonial mitotic chromosome sets and diploid, hypo- or hypertetraploid, tetraploid, and octoploid chromosome numbers were detected. In turn, 14.6% of males showed a heterozygous deficiency of chromatin in L2 and M3 bivalents in addition to the presence of B chromosomes.

Heavy metal concentrations in Enchytraeidae (Oligochaeta) in the Niepolomice Forest.

Enchytraeidae were investigated as a part of a broad study of Niepolomice Forest ecosystem functioning. Animals were collected in oak-hornbeam (Tilio-Carpinetum) and in mixed oak-pine (Pino-Quercetum) stands. The highest cadmium concentration was observed in oak-hornbeam from the northern part of the forest (32.33+/-23.22 mg kg(-1) dry mass), with lower ones in mixed oak-pine (13.60+/-21.42 mg kg(-1) dry mass) and southern oak-hornbeam stands (11.84+/-7.52 mg kg(-1) dry mass). Lead concentrations were higher in mixed oak-pine (47.73+/-23.36 mg kg(-1) dry mass) and southern oak-hornbeam (52.20+/-45.19 mg kg(-1) dry mass) stands than in northern, oak-hornbeam (9.20+/-6.46 mg kg(-1) dry mass). The copper concentration was similar in animals from all forest types studied (147.94+/-106.38 mg kg(-1) dry mass in mixed oak-pine, 138.35+/-64.76 mg kg(-1) dry mass in northern oak-hornbeam, and 138.27+/-80.08 mg kg(-1) dry mass in southern oak-hornbeam). The lowest concentration of zinc in animals was found in northern oak-hornbeam (1015.27+/-300.28 mg kg(-1) dry mass) and higher concentrations, in mixed oak-pine (2233.81+/-1825.41 mg kg(-1) dry mass) and southern oak-hornbeam (2366.60+/-1987.52 mg kg(-1) dry mass). The concentrations of available metals in litter trended with those in the animals, but only the relation between lead concentration in animals and available lead in litter was significant. The spatial distribution of metal concentrations in the whole forest suggests a relationship between forest type and the content of cadmium, lead, and zinc in animals. The spatial distribution of heavy metal content in enchytraeids was investigated in selected plots as well. The coefficients of variation indicated that lead had the most diverse distribution and zinc the most homogeneous.

Effect of heavy metals and storage time on two types of forest litter: basal respiration rate and exchangeable metals.

Two types of forest litter, MOR and MULL, were treated with 0 (control), 25, 100, 400, 1600, and 6400 mg kg-1 Cd, Cu, Pb, or Zn after different storage times (35, 75, and 125 days at approx 5 degreesC). Highly significant effects on respiration rate were observed for dose of heavy metals, type of litter, type of metal, and storage time. The respiration rate of untreated MULL litter was lower than that of untreated MOR in all incubations, and the slope of the relation to the dose of metals was steeper for MOR. Respiration rates after storage were lower than in fresh litter, and the slope of the relation between respiration rate and metal dose was less steep after storage. In the first incubation, MULL litter was more sensitive to Cd, Cu, and Pb and less sensitive to Zn than MOR litter. After 125 days of storage, no single significant effect was found in MULL litter, whereas in MOR litter all metals still inhibited respiration rate significantly. The relative toxicity of metals was similar for both litter types, and the average EC50respiration values were (mg kg-1) Cu=3880, Zn=5610, Cd=6320, and Pb=24800. The percentages of exchangeable metals (1 M NH4OAc, pH=7) in MULL litter were lower on average than in MOR litter, and the order of solubility of the metals was Cd>Zn>Pb>Cu. Storage caused no significant difference in the average percentage of exchangeable metal. The highest doses of heavy metals increased the amounts of Ca, K, Mg, and Na extracted.

Effect of heavy metals and mineral nutrients on forest litter respiration rate.

Two hypotheses were tested: (1) heavy metals such as Zn, Pb and Cd can suppress the respiration rate of forest litter at low-moderate pollution levels, and (2) mineral nutrients such as K, Ca and Mg can counteract the toxicity of heavy metals when applied onto the polluted litter. In a completely randomised design, three doses of heavy metals were used: Cd-10, 50, 250; Pb-100, 500, 2500; Zn-200, 1000, 5000 microg/g dwt litter, respectively. For Ca, Mg and K, the doses corresponded to 100, 500 and 2500 microg/g. A significant decrease in cumulated CO2 evolution after 4 weeks of incubation was found for the litter samples treated with medium doses of Zn, Cd/Ca, Cd/Mg, Pb/Ca, Pb/Mg, Pb/K, Zn/Mg, Zn/Ca, Zn/K and for all the highest-dose treatments. The largest drop in respiration rate in both the medium and the highest doses was caused by additions of Zn either alone or in combination with K, Ca or Mg. The additions of mineral nutrients were found to decrease the litter respiration rate below the value measured for the respective heavy metal alone in the case of Cd/Ca, Cd/Mg, Pb/Ca, Pb/Mg and Pb/K in the medium-dose treatments, and for Cd/Ca, Cd/Mg, Cd/K, Pb/Ca and Pb/Mg in the highest-dose treatments. In all other cases, additions of the mineral nutrients did not influence the respiration rate significantly when compared to the effect of the respective heavy metal.