ABSTRACT
Rhaponticum unifl orum (L.) and Serratula centauroides (L.) are prospective adaptogenic ecdysteroid-containing plants of Buryatia Republic. Underground organs of Rhaponticum unifl orum (L.) and the grass of Serratula centauroides (L.) may be suggested as a plant material containing ecdysteroids for creation of new adaptogenic remedies. Key words: ecdysteroid, Rhaponticum unifl orum (L.), Serratula centauroides (L.) Adaptogenes possess a specifi c combination of peculiar pharmacological properties: being non-toxic substances these preparations manifest anabolic properties which set condition for physiological stimulation of the protective system functions in the body, i.e. bring the body to the state of non-specifi c increased resistance. Adaptogenes have stimulating effect and promote the increase of the resistance to various unfavorable factors. It is worthy to note that adaptogenic properties are characteristic features of natural preparations or compounds being natural metabolites. Some polysaccharides, vaccines, as well as synthetic preparations – stimulators with “economizing” type of the effect have certain adaptogenic properties which are manifested in the regulation of immune system functions. Nevertheless, specifi c stimulating properties and preventive effect manifested in the preparation of the bodily systems to overloading are the most peculiar features of typical adaptogenes [1,2]. At present the use of ecdysteroid-containig plants for developing new adaptogenic medicinal preparations and tonics are of great interest. Plant ecdysteroids have practical signifi cance: they are contained almost in all plants but the difference in concentration levels reaches 8-9 orders. Only few species are characterized by high content of ecdysteroids. Rhaponticum unifl orum (L.) and Serratula centauroides (L.) are ecdysteroid-containing plants growing in the territory of Buryatia [3]. The aim of the present work is to study the chemical composition of ecdysteroid-containing plants - Rhaponticum unifl orum (L.) and Serratula centauroides (L.).
ABSTRACT
Topicality: In recent years interest to biologically active preparations of a phytogenesis is considerably increased. Tannins are very widespread group of biologically active agents of the plants, having various pharmacological properties (astringent, styp tic, antiinflammatory), and causing their broad application in m edicine.[1] Therefore the problem of determination of high quality of medicines and the medicinal plant raw materials containing this group of biologically active agents is very actual. The techniques, allowing to establish high quality of the raw materials containing tannins, were applied to raw materials of Rhizomata Bergeniae crassifoliae from an original derivative plant of a Bergenia crassifolia (L. ) Fritsch, genus of Bergenia. The purpose of this work: is to carry out a number of research works on studying of a chemical composition of the medicinal plant raw materials containing tannins, and to make a precise characteristic of these plants on the basis of the received results. According to a goal of this research the following tasks are defined: 1. To carry out the macroscopic and microscopical analysis of medicinal raw materials of Rhizomata Bergeniae crassifoliae 2. To define the main indicators of commodity research;3. To define quality and quantity biologically active agents in these plants. For the solution of tasks the following Methods: of the analysis were used:macroscopic, microscopical, phytochemicaland histochemical analysis, thin-layerchromatography and spectophotometery.Powder of Rhizomata Bergeniae crassifoliae(Krasnogorseleksredstva ltd. production) and crumbled of Rhizomata Bergeniae crassifoliae (the manufacturer of Ivan-Chay ltd.) were used as samples for this analysis.Results: According to pharmacopeia article “ Rhizomata Bergeniae crassifoliae “, for the specified raw materials are established the following numerical indicators: quantity tannins not less than 20%; humidity no more than 14%; total ash no more than 4%; ashes, insoluble in 10% Acidum hydrochloricum solution, no more than 0,5%. [2] The analyzed raw materials correspond to these indicators. As a result of carrying out histochemical and phytochemical reactions in rhizomes of a bergenia the following biologically active agents were found: arbutin, tannins (catechol, EGCG), polysaccharides.Conclusion: Medicinal plant raw materials ofKrasnogorseleksredstva ltd. and Ivan-Chay ltd.correspond to the general pharmacopeia article“Rhizomata Bergeniae crassifoliae”.References1. L.M. Fedoseeva. Study of tannins and aboveground vegetative organs Bergeniae crassifoliae (L. ) Fritsch native of Altai / L.M. Fedoseeva, E.V. Timochin // Chemistry of plant raw material. – 2003. - №1. – pp.73-77.2. State pharmacopeia Ed. XI. M.: Medicine, 1990. T.2. p. 399.3. State pharmacopeia Ed. XI. M.: Medicine, 1987. T.1. p. 335.4. G.P. Yakovlev. Rhizomata Bergeniae crassifoliae / G.P. Yakovlev, I.A. Samilina, L.V. Moshkova // Pharmacognozy. SPb.: SpecLit, 2006. pp. 556-558.5. I.M. Korenskaya. Qualitative and quantitativeanalysis of medicinal plant raw materials containing tannins. / I.M. Korenskaya, N.P. Ivanovskaya, I.E. Izmalkova // Medicinal plants and medicinal plant raw materials containing simple phenols, lignans, tannins. Voronezh, 2007. Pp. 48-51Methods: of the analysis were used: macroscopic, microscopical, phytochemical and histochemical analysis, thin laye r chromatography and
ABSTRACT
The effects of different iron chelators on both OH.-generation in Fenton reaction and lipid peroxidation products accumulation in brain homogenate were studied. Intensity of luminol-dependent chemiluminescence was the measure of OH.-radical generation. The rate of lipid peroxidation was estimated as malondialdehyde accumulation. It was shown, that inhibition of chemiluminescence by the tested substances increased in next series: ADP, polyhydroxamic acid (PHA), EDTA, desferal, o-phenanthroline, ascorbate. Inhibition of lipid peroxidation by these substances increased in other order: ADP, Ascorbate, EDTA, PHA, desferal, o-phenanthroline. The results obtained allow to conclude, that antioxidant activity of ascorbate is due to the trapping of OH. radicals; in contrast, inhibition of the free radical processes by other tested substances is due to binding of iron ions. Antioxidant effect of PHA is almost as strong as effects of desferal, o-phenanthroline and EDTA. The lowest antioxidant activity was found in the presence of ADP.
