ABSTRACT
A method of extracorporeal cultivation and fertilization of human oocytes is described. A special method of selection of active spermatozoa was used. The zygote formed 24-36 hours after the fertilization of oocytes; cleavage of two blastomeres occurred in 36-40 hours. Formation of the four-cell stage embryos was observed in 48 hours. The number of blastomeres reached eight in 60-64 hours and the morula formed in 100-120 hours. The results obtained in this work open opportunities for wide study of physiology and pathology of early human embryonic development.
Subject(s)
Embryo, Mammalian/physiology , Fertilization , Embryology/methods , Humans , In Vitro TechniquesABSTRACT
The egg-cells were obtained from ovaries removed for different clinical reasons and cultivated after Edward's technique with modifications to complete the maturation division. The process of fecundation, formation of pronucleuses, separation of the 2nd pole body and the first stage of egg-cell cleavage (up to the stage of 3 blastomeres). Non-fertilized egg-cells underwent random fragmentation into blastomere-like nucleus-free particles.
Subject(s)
Fertilization , Ovum/physiology , Cell Division , Cells, Cultured , Female , Haploidy , Humans , In Vitro Techniques , Male , Meiosis , Mitosis , Oocytes/physiology , Pregnancy , Sperm-Ovum Interactions , Time Factors , Zygote/physiologySubject(s)
Fertilization , Ovum , Culture Media , Female , Humans , In Vitro Techniques , Male , Ovum/physiology , Spermatozoa , Time FactorsABSTRACT
PIP: 47 pubescent rats were inserted with IUDs and then impregnated. Ova were removed at 2, 3, 4, 5, and 6 days after impregnation and examined microscopically. The IUD failed to interrupt the processes of fertilization and of cleavage of the ova during their passage along the oviduct or the rate of passage. When a blastocyst entered the uterine cornu with an IUD, degenerative changes appeared leading to its death be fore the development of decidual transformations in the endometrial stroma.^ieng
