ABSTRACT
Seven strains of an unknown Gram-positive catalase-negative chain-forming coccus-shaped organism isolated from clinical specimens from sheep were characterized by phenotypic and molecular taxonomic methods. Comparative 16S rRNA gene sequencing studies demonstrated that the bacterium represents a new sub-line within the genus Streptococcus. The unknown bacterium was readily distinguished from recognized streptococcal species by biochemical tests and electrophoretic analysis of whole-cell proteins. Based on phylogenetic and phenotypic evidence, it is proposed that the unknown bacterium be classified as Streptococcus ovis sp. nov. The type strain of Streptococcus ovis is CCUG 39485T (= LMG 19174T).
Subject(s)
Phylogeny , Sheep Diseases/microbiology , Streptococcus/classification , Animals , Arthritis, Infectious/microbiology , Arthritis, Infectious/veterinary , Bacterial Proteins/analysis , Molecular Sequence Data , Pneumonia, Bacterial/microbiology , Pneumonia, Bacterial/veterinary , Sheep , Streptococcal Infections/microbiology , Streptococcal Infections/veterinary , Streptococcus/genetics , Streptococcus/isolation & purificationABSTRACT
Three strains of a previously undescribed Actinomyces-like bacterium were isolated from human clinical specimens. Phenotypic studies indicated that the strains were members of the genus Actinomyces and were presumptively identified as Actinomyces turicensis. Comparative 16S rRNA gene sequencing studies showed that although the bacterium is phylogenetically closely related to Actinomyces turicensis, it nevertheless constitutes a new sub-line within the genus Actinomyces. Based on phenotypic and molecular chemical and molecular genetic evidence, it is proposed that the unknown Actinomyces-like bacterium from human clinical specimens be classified as Actinomyces funkei sp. nov. The type strain of Actinomyces funkei is CCUG 42773T (= CIP 106713T).
Subject(s)
Actinomycetales/classification , Phylogeny , Actinomycetales/genetics , Actinomycetales/isolation & purification , Adult , Cefuroxime/therapeutic use , DNA, Bacterial/genetics , DNA, Ribosomal/genetics , Drug Therapy, Combination/therapeutic use , Endocarditis, Bacterial/complications , Endocarditis, Bacterial/drug therapy , Female , Humans , Molecular Sequence Data , Phenotype , RNA, Bacterial/genetics , RNA, Ribosomal, 16S/genetics , Staphylococcal Infections/complications , Staphylococcal Infections/drug therapy , Substance Abuse, Intravenous/complicationsABSTRACT
Four strains of a hitherto unrecognized gram-positive, catalase-negative, facultatively anaerobic, rod-shaped bacterium isolated from human sources were characterized using phenotypic and molecular taxonomic methods. Comparative 16S rRNA gene sequencing demonstrated that the bacterium represents a new subline within the Lactobacillus casei/Pediococcus rRNA group of the genus Lactobacillus. The unknown bacterium was readily distinguished from all other described Lactobacillus species and related taxa by biochemical tests and electrophoretic analysis of whole-cell proteins. On the basis of phylogenetic and phenotypic evidence, it is proposed that the unknown bacterium be classified as Lactobacillus coleohominis sp. nov. The type strain of Lactobacillus coleohominis is CCUG 44007T (= CIP 106820T).
Subject(s)
Lactobacillus/classification , Lactobacillus/genetics , Phylogeny , Adult , Cervix Uteri/microbiology , DNA, Ribosomal/genetics , Female , Humans , Lactobacillus/isolation & purification , Lactobacillus/physiology , Middle Aged , Molecular Sequence Data , Phenotype , RNA, Ribosomal, 16S/genetics , Urine/microbiology , Vagina/microbiologyABSTRACT
Two strains of a previously undescribed Actinomyces-like bacterium were recovered in pure culture from infected root canals of teeth. Analysis by biochemical testing and polyacrylamide gel electrophoresis of whole-cell proteins indicated that the strains closely resembled each other phenotypically but were distinct from previously described Actinomyces and Arcanobacterium species. Comparative 16S rRNA gene-sequencing studies showed the bacterium to be a hitherto unknown subline within a group of Actinomyces species which includes Actinomyces bovis, the type species of the genus. Based on phylogenetic and phenotypic evidence, we propose that the unknown bacterium isolated from human clinical specimens be classified as Actinomyces radicidentis sp. nov. The type strain of Actinomyces radicidentis is CCUG 36733.
Subject(s)
Actinomyces/classification , Actinomyces/isolation & purification , Actinomycosis/microbiology , Dental Pulp Cavity/microbiology , Actinomyces/genetics , Actinomyces/metabolism , Adult , Aged , Aged, 80 and over , Female , Genes, Bacterial , Genes, rRNA , Humans , Male , Molecular Sequence Data , Phylogeny , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNAABSTRACT
Three strains of a previously undescribed Actinomyces-like bacterium were isolated from human clinical sources (urine, urethra and vaginal secretion). Biochemical testing and PAGE analysis of whole-cell proteins indicated that the strains were phenotypically homogeneous and distinct from previously described Actinomyces and Arcanobacterium species. Comparative 16S rRNA gene sequencing studies showed the bacterium to be a hitherto unknown subline within a group of Actinomyces species which includes Actinomyces bovis, the type species of the genus. Based on phylogenetic and phenotypic evidence it is proposed that the unknown bacterium from humans be classified as Actinomyces urogenitalis sp. nov. The type strain of Actinomyces urogenitalis is CCUG 38702T (= CIP 106421T).
Subject(s)
Actinomyces/classification , Urogenital System/microbiology , Actinomyces/chemistry , Actinomyces/genetics , Adult , Aged , Bacterial Proteins/analysis , DNA, Bacterial/analysis , DNA, Ribosomal/analysis , Female , Humans , Male , Molecular Sequence Data , Phylogeny , RNA, Bacterial/genetics , RNA, Ribosomal, 16S/geneticsABSTRACT
Biochemical, molecular chemical and molecular genetic studies were performed on an unknown Gram-positive, catalase-negative, chain-forming coccus isolated from the urine of a patient suffering from cystitis. Comparative 16S rRNA gene sequencing showed that the organism is a member of the 'pyogenic subgroup' of the genus Streptococcus and has a close affinity with Streptococcus pyogenes and Streptococcus canis. The unknown coccus was, however, readily distinguished from these species and other streptococci by biochemical tests and electrophoretic analysis of whole-cell proteins. Based on phenotypic and phylogenetic evidence, it is proposed that the unknown bacterium be classified as a new species of the genus Streptococcus, Streptococcus urinalis sp. nov. The type strain of Streptococcus urinalis is CCUG 41590T.
Subject(s)
Cystitis/microbiology , Streptococcal Infections/microbiology , Streptococcus/classification , Urine/microbiology , DNA, Bacterial/genetics , DNA, Ribosomal/genetics , Electrophoresis, Polyacrylamide Gel , Female , Genes, rRNA , Humans , Middle Aged , Molecular Sequence Data , Phenotype , Phylogeny , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Streptococcus/chemistry , Streptococcus/genetics , Streptococcus/isolation & purificationABSTRACT
An unusual catalase-positive pleomorphic Gram-positive rod isolated from a human clinical specimen was subjected to a polyphasic taxonomic analysis. Comparative 16S rRNA gene sequence analysis revealed the unknown bacterium was a member of the high G+C branch of the Gram-positive bacteria (Actinobacteria), and was phylogenetically a member of the family Propionibacteriaceae, with Luteococcus japonicus as its nearest relative. Based on both phenotypic and phylogenetic evidence, it is proposed that the unknown bacterium be classified in the genus Luteococcus, as Luteococcus peritonei sp. nov. The type strain of Luteococcus peritonei is CCUG 38120T.
