ABSTRACT
UNLABELLED: AIM. Study the effect of fluconazole antimycotic on temporal organization of biological properties of Candida albicans. MATERIALS AND METHODS: Clinical isolates and a museum C. albicans strain were used. The study was carried out for 24 hours with a 4 hour interval, determining proliferative, adhesive, morphological, protease, catalase, phospholipase activity. The results of the study were treated statistically. RESULTS: Maximal sensitivity to fluconazole was established for the museum C. albicans strain during the day, in clinical isolates--in the evening and at night. After the effect of 1/2 minimal inhibitory concentration (MIC) of the preparation, the rhythms of all the parameters changed in the museum culture from circadian to ultradian: The preparation reduced mesor and amplitude of the oscillation of proliferative and adhesive activity, but did not have an effect on chrono-infrastructure of morphogenesis and aggression enzymes. The temporal rows of biological activity of pathogenic C. albicans isolates after the incubation with fluconazole were characterized by synchronization of all the properties in a narrow temporal interval from 07:00 to 11:00 and from 16:00 to 23:00 hours. CONCLUSION: The results obtained give evidence on the lability of adhesion factors and colonization and stability of virulence factors in the museum variant. A clear synchronization in time of all the pathogenicity factors of clinical isolates gives evidence on the economy of material and energy resources, opening a perspective of using the chronobiological approach in antibiotics therapy of candidoses.
Subject(s)
Antifungal Agents/pharmacology , Biological Clocks/drug effects , Candida albicans/metabolism , Cell Proliferation/drug effects , Fluconazole/pharmacology , Fungal Proteins/metabolism , Dose-Response Relationship, Drug , PhotoperiodABSTRACT
AIM: Study the effect of exometabolites of associative microbiota on circadian dynamics of functional parameters, that reflect pathogenic and persistence properties of fungi and bacteria. MATERIALS AND METHODS: Clinical isolates of Candida albicans, isolated-from intestine of healthy individuals and patients with candidosis, as well as clinical isolates and museum ATCC strains Staphylococcus. aureus 25923, Escherichia coli 35218 and Pseudomonas aeruginosa 27853 were taken for study of proliferative, adhesive, catalase, protease, phospholipase, hemolytic, anti-lysozyme, biofilm-forming activity. The results were treated statistically. RESULTS: C. albicans isolates, isolated from healthy individuals were revealed to be indifferent to the effect of bacterial metabolites. Chrono-infrastructure of biological properties of fungi altered under the effect of microbiota metabolites. Hospital isolates of S. aureus, E. coli and P. aeruginosa displayed a relative stability of physiological properties against the effect of bacterial-fungal metabolites as opposed to museum strains. CONCLUSION: The alterations of chrono-infrastructure of biological rhythms of microorganisms by bacterial-fungal metabolites of associants reflect the intensity of the biological system, that is inevitable during the process of formation of inter-microbial interactions.
Subject(s)
Candida albicans/metabolism , Escherichia coli/metabolism , Microbial Consortia , Pseudomonas aeruginosa/metabolism , Staphylococcus aureus/metabolism , Candida albicans/isolation & purification , Escherichia coli/isolation & purification , Humans , Pseudomonas aeruginosa/isolation & purification , Staphylococcus aureus/isolation & purificationABSTRACT
AIM: Evaluation of the influence of bacterial microbiota exometabolites on temporal organization of biological features of Candida albicans. MATERIALS AND METHODS; Experiments were carried out on the model of C. albicans 24433 ATCC for 24 hours with 4 hour intervals. Biorythms of proliferative, phospholipase and adhesive activity of fungi were studied. Influence of exometabolites of Staphylococcus aureus 25923 ATCC, Escherichia coli 35218 ATCC, Pseudomonas aeruginosa 27853 ATCC on biological properties of C. albicans were examined. The results were statistically analyzed by using Student's t-test. RESULTS: Circadian biological rhythms were detected for all investigated parameters of C. albicans 24433 ATCC. In the presence of exometabolites of S. aureus and P. aeruginosa, rhythm profile and amplitude oscillation of the fungi changes, and phase synchronism is disrupted. Under the influence of exometabolites of Gram-negative microbiota a marked desynchrony of fungi adhesive activity was observed. CONCLUSION: The results obtained confirm the key role of microorganisms in the formation and regulation of pathogenic microsymbiocenosis, and determine the necessity to study mechanisms of symbiotic interaction of bacteria and fungi.
Subject(s)
Candida albicans/pathogenicity , Escherichia coli/metabolism , Pseudomonas aeruginosa/metabolism , Staphylococcus aureus/metabolism , Symbiosis , Candida albicans/enzymology , Cell Adhesion , Circadian Rhythm , Metagenome , Phospholipases/metabolismSubject(s)
Bacterial Infections , Fishes/parasitology , Opisthorchis/microbiology , Opisthorchis/physiology , Proteobacteria/physiology , Symbiosis , Animals , Anti-Bacterial Agents/pharmacology , Bacterial Infections/physiopathology , Bacterial Infections/transmission , Colony Count, Microbial , Disease Vectors , Drug Resistance, Bacterial , Life Cycle Stages , Microbial Sensitivity Tests , Proteobacteria/classification , Proteobacteria/drug effects , Proteobacteria/isolation & purification , Proteobacteria/pathogenicity , VirulenceABSTRACT
In series of experiments intraday dynamics of proliferative activity of museum and hospital strains of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa was revealed. Statistically significant circadian and ultradian biorhythms in these strains were detected. Chronoinfrastructure of hospital strains differed from museum ones. This finding allows to detect hospital strains of these bacteria.
