[The European approaches to the microbiological food safety assessment].

The purpose of the study was the analysis of the results of the study of the quality of food products and environment of food industries on microbiological indicators according to domestic and European regulations (EU). The analysis of the quality of food, studied during 2008-2012, showed an increase in the number of samples that do not meet the requirements of regulations at the same time on several factors: mesophilic aerobes and facultatively anaerobic microorganisms, bacteria of the family Enterobacteriaceae and fungi (molds and yeasts). The results of research in dining facilities, food industries and distribution network for 2008-2011. indicate that in the swabs used for sampling surfaces the incidence of sanitary-indicative microorganisms ranged from 2.91 to 8.87%. There was absent dynamics of the improvement in the sanitary-epidemiological status of institutions for food processing. The highest rate of contamination (81.1-82.4%) was observed on the surfaces of tables, dishes, utensils, equipment, and most positive samples were represented by bacteria of the family Enterobacteriaceae. There was performed a critical analysis of European approaches to the study of food. It was made a draw about premature introduction of the Order of the Ministry of Health of Ukraine No 548 dated 19.07.2012, the "Microbiological Criteria for detection of safety of food products," based on the provisions of Regulation number 2073/2005 in Ukraine.

[Drinking water regulations in Ukraine and infectious morbidity rate with water pathway of transmission of causative agents].

These are presented drinking water regulations in Ukraine on microbiological indices in the new state document "Hygienic Drinking water regulations intended for human consumption" (State sanitary regulations and standards (GsanPiN) 2.2.4-171-10). There is reported an analysis of both the open waterbodies water quality and water for centralized water supply in 2007-2011 and 1992-1996 also on sanitary-bacteriological indices for performance of comparative retrospective analysis. There has been shown water quality degradation for open waterbodies, the main sources of water supply for population. Against this background, a marked improvement of drinking water quality has been noted, the number of non-standard samples decreased to 3.6 % compared with 8.8% in the preceding 15 years. This index correlates with a decrease in the number of outbreaks of infections transmitted by water. There was noted unsatisfactory registration of viral infections with water pathway of transmission and the increase in morbidity rate of gastroenterocolitides of unknown etiology that may include acute viral intestinal infections. Expediency to keep "fermentation of glucose" test in bacteriological examination of water in the identification scheme of general coliforms has been substantiated.

[The study of antimicrobial properties of silver nanoparticles in the form of a colloidal solution in the matrix of finely dispersed silica].

In the experimental study obtained with chemical method colloid solution of nanoparticles (NPs) of silver (Ag) and a composite on his base in the matrix of finely dispersed silica with particle size of 8-12 nm and NPs concentration in basic solution of 0,0016% (0,016 mg/cm3) were established to exhibit high antimicrobial activity against the test organisms: E. coli, P. aeruginosa, S. Aureus and C. Albicans, which depended on a set of factors. Antibacterial properties of tissue impregnated with Ag-NPs were studied. As stabilizing substances a mixture of surface-active substance sodium dodecyl sulfate and polymer polyvinylpyrrolidone was used Before the beginning of the study effective neutralizer was tailored. Times of preservation of antimicrobial activity of test samples have been established, and also their stability throughout long term of supervision (24 months) has been shown. Effect of organic pollution on antimicrobal activity of the samples has been studied. Based on obtained results the algorithm of the study of antimicrobial properties of nanopreparations has been elaborated.

[Study of the mechanisms of interaction between intestinal bacteriophages and polyaluminum coagulants].

The authors have investigated the adsorption-elution of intestinal bacteriophages T2 and MS2 as a model of enteric viruses, by using the coagulants aluminum oxychloride (AOX78) and aluminum sulfate (AS). The investigation has ascertained the effective removal of the phages from water with AOX78 (98.7-100%) and the low efficiency of elution of coliphages from the particles of AOX hydrolysis products (0.1-3.7% of the baseline count of bacteriophages), which may suggest that AOX has antiviral activity. The above phenomena produced by AS are less evident.

[Problem of pathogenesis of Mycoplasma infection]. / K voprosu o patogeneze mikoplazmennoi infektsii.

It has been shown that irrespective of the primary focus of affliction, M. hominis-induced infection should be regarded as a systemic disease characterized by an immune-mediated inflammation which is contributed to by blood kallikrein-kinin system activation resulting in changes in the coagulative system and fibrinolysis until disseminated intravascular coagulation develops. This allows one to interpret in a new fashion the etiology of some diseases proceeding with signs of vasculitis.

[Dynamics of Mycoplasma infection in patients with glomerulo- nephritis during pathogenetic therapy]. / Dinamika mikoplazmennoi infektsii u bo'lnykh glomerulonefritom pod vliianiem patogeneticheskoi terapii.

Among bacterial infections arising in glomerulonephritis and complicating its course Mycoplasma infection (M. hominis) is not a rare finding as shown by microbiological and serological examinations. Good therapeutic results achieved in patients treated with prednisolone and cyclophosphamide suggest inhibiting action of glucocorticoids on Mycoplasma infection.

[Changes in the biological properties of Mycoplasma hominis under the influence of prednisolone and cyclophosphane]. / Izmenenie biologicheskikh svoistv Mycoplasma hominis pod vliianiem prednizolona i tsiklofosfana.

The results of experimental investigations on the influence of prednisolone and cyclophosphane on biological properties of M. hominis are presented. Changes in the morphology of mycoplasmal colonies depending on the type and dose of the preparation were stated. The virulence of mycoplasmas was found to be intensified with an increase of the concentration of prednisolone in the culture medium from 0.1 to 20.0 micrograms/ml and that of cyclophosphane from 0.2 to 20.0 micrograms/ml.