[Intestinal microflora in young children with rotavirus infection]. / Mikroflora kishechnika u detei rannego vozrasta s rotavirusnoi infektsiei.

A total of 270 children with rotavirus diarrhea were examined. The quantitative and qualitative composition of their intestinal microflora was studied. Most frequently microorganisms of the genus Enterobacter and most seldom, Serratia were isolated. A decrease in the amount of bifidobacteria, normal Escherichia coli and an increase in the amount of lactose-negative Escherichia were noted. In cases of pronounced dysbiosis in young children the clinical course of rotavirus infection is aggravated and the period of rotavirus excretion is prolonged.

[A method of detecting the rotavirus antigen in feces]. / K metodam vyiavleniia rotavirusnogo antigena v fekaliiakh.

Counter-current immunoelectrophoresis (CCIEP) has been employed for indication of a rotaviral antigen. 50% feces homogenate in physiologic saline, clarified by centrifugation, has been used in the test, as well as a highly active antiserum of rabbits immunized with calf diarrhea (Nebraska strain) rotavirus. The results of parallel studies of 103 feces samples collected from children with acute gastroenteritis, carried out by CCIEP and electron microscopy, coincided in 84.5% of cases. The authors have examined the rotaviral antigen detection rate in children and adults suffering from acute gastroenteritis and in subjects who have had contacts with them; they have analyzed the periods when the antigen could be isolated from the feces and the relationship between the antigen detection rate, the patients' sex, and season. CCIEP is recommended to be widely employed for the detection of the rotaviral antigen in feces.

[Clinico-laboratory analysis of the diarrhea syndrome in young children excreting rotaviruses with feces]. / Kliniko-laboratornyi analiz diareinogo sindroma u detei rannego vozrasta, vydeliaiushchikh rotavirus s fekaliiami.

A total of 404 children admitted to the infectious hospital of the non-intestinal type were examined with the aid of counter immunoelectroosmophoresis. Rotavirus antigen was detected in feces in 46.8% of 126 children admitted with a diagnosis of diarrhea, in 10.8% of 278 without diarrhea, and also in 32.9% of 149 children with diarrhea that occurred at the hospital. The clinical course of acute rotavirus gastroenteritis appeared to be materially influenced by acute respiratory viral infection, particularly influenza, and by certain types of opportunistic bacteria contained in high concentrations by the intestine.