Laboratory and field evaluation of two formulations of Bacillus thuringiensis M-H-14 against mosquito larvae in the Islamic Republic of Iran, 2012.

Due to low efficacy in the field of a local commercial product of Bacillus thuringiensis M-H-14 (Bioflash(®)), a study was designed to assess its efficacy in laboratory, glass standard aquarium, semi-field and field conditions at both target and higher dosages against immature stages of Anopheles spp. and Culex spp. In laboratory conditions, the LC50 values of wettable powder and granule formulations were 227 and 1031 ppm respectively against a susceptible strain of An. stephensi. Following application of wettable powder and granules at 56.1 mg/aquarium, the survival rates of the exposed larvae to the granule formulation were 65.6% and 54.2% on days 6 and 8 respectively. In the artificial ponds, the larval density was reduced to 38.9%, 39.3% and 65.1% at dosages of 2, 4 and 8 g/m(2) respectively. In rice fields, at a dosage of 2 kg/ha, the density of immature larvae were reduced to 33.1% and 28.6% 7-days post-treatment. Further investigations are needed for the reasons for the low efficacy of this larvicide.

Laboratory and field evaluation of two formulations of Bacillus thuringiensis M-H-14 against mosquito larvae in the Islamic Republic of Iran, 2012

Due to low efficacy in the field of a local commercial product of Bacillus thuringiensis M-H-14 [Bioflash[registered sign], a study was designed to assess its efficacy in laboratory, glass standard aquarium, semi-field and field conditions at both target and higher dosages against immature stages of Anopheles spp.and Culex spp.In laboratory conditions, the LC[50]values of wettable powder and granule formulations were 227 and 1031 ppm respectively against a susceptible strain of An.stephensi.Following application of wettable powder and granules at 56.1 mg/aquarium, the survival rates of the exposed larvae to the granule formulation were 65.6% and 54.2% on days 6 and 8 respectively.In the artificial ponds, the larval density was reduced to 38.9%, 39.3% and 65.1% at dosages of 2, 4 and 8 g/m[2]respectively.In rice fields, at a dosage of 2 kg/ha, the density of immature larvae were reduced to 33.1% and 28.6% 7-days post-treatment.Further investigations are needed for the reasons for the low efficacy of this larvicide

في ضوء تضاؤل الكفاءة للمنتج التجاري المحلي المستحضر من العصية الثورنجية صمم الباحثون دراسة لتقييم كفاءته في المختبر ضمن حوض مائي زجاجي معياري، وفي ظرف ميدانية وشبه ميدانية لكل من الجرعات المستهدفة والجرعات الأعلى ضد المراحل غير الناضجة من أنواع الأنوفيلات وأنواع الباعضات. وفي الظروف المختبرية، كانت قيم الجرعة القاتلة للنصف من مستحضرات المسحوق المرطب جزءا بالمليون ومن الحبيبات جزءا بالمليون، ضد الذراري المستجيبة من الأنوفيلة الأسطفانية. وبعد تطبيق المسحوق المرطب والحبيبات على حوض مائي بمقدار 65.1 مغ/حوض وجد الباحثون أن معدل بقاء اليرقات على قيد الحياة بعد تعرضهن لمستحضر الحبيبات كان 65.6 % في اليوم 6، و 54.2 % في اليوم 8. أما في الأحواض الاصطناعية فإن كثافة اليرقات انخفضت إلى 38.9 % بجرعة 2 غرام/متر مربع، وإلى 39.3 بجرعة 4 ميلي غرام، وإلى 65 % بجرعة 8 غرام/متر مربع. وفي حقول زراعة الأرز فقد أدت جرعة مقدارها 2 كيلو غرام/هيكتار إلى انخفاض كثافة اليرقات غير الناضجة إلى 33.1 % و 28.6 % بعد مرور 7 أيام على المعالجة. وهناك حاجة لمزيد من الدراسات حول أسباب انخفاض كفاءة مبيدات اليرقات

En raison de la faible efficacité sur le terrain d'un produit commercial local de Bacillus thuringiensis M-H-14 [Bioflash[R], une étude a été élaborée pour évaluer son efficacité en laboratoire, en aquarium de verre standard ainsi que dans des conditions déterraient de semi-terrain à des doses cibles mais aussi à des doses supérieures contre des Anopheles spp.et des Culex spp.à des stades immatures.Aux conditions de laboratoire, les valeurs de la CL[50]pour la poudre mouillable et les formulations en granulés étaient de 227 et 1031 ppm respectivement sur une souche sensible d'An.stephensi.Après application de poudre mouillable et de granules à 56, 1 mg/aquarium, les taux de survie des larves exposées à la formulation en granules étaient de 65, 6 % et 54, 2 % aux jours 6 et 8 respectivement.Dans les plans d'eau artificiels, la densité larvaire a été réduite, passant à 38, 9 %, 39, 3 % et 65, 1 % à des doses de 2 g/m[2], 4 g/m[2]et 8 g/m[2]respectivement.Dans les rizières, à une dose de 2 kg/ha, la densité des larves immatures a été réduite, passant à 33, 1 % et 28, 6 %, sept jours après le traitement.Des recherches supplémentaires sont nécessaires pour expliquer la faible efficacité de ce larvicide

Outcome of primary childhood central nervous system tumors: results from a single center in Iran.

BACKGROUND: Childhood primary central nervous system (CNS) malignancies are one of the most important concerns in pediatric oncology. MATERIAL: In this historical cohort, 82 patients treated in Ali-Asghar Children's Hospital between 1996 and 2005 were evaluated for age, gender, type of treatment, tumor pathology, and survival. RESULTS: The records of 38 female (46.3%) and 44 male (53.7%) patients were included. The mean age was 6.8 ± 3.39 years (6 months to 14 years); the frequency of medulloblastoma was significantly more than others (p = 0.012). Complete resection, radiotherapy, and chemotherapy were done in 47.5, 82.9, and 97.56%, respectively. Estimated 5 years overall and event-free survivals (EFSs) were approximately 68.5 and 35%, respectively. The patients with complete resected tumors had significantly better outcome (p = 0.013). Also, low-grade versus high-grade astrocytoma was significantly a better outcome (p = 0.01). CONCLUSION: The poor EFS with consideration of high number of progressive disease among our patients (50% unresectable tumor) suggests that children in Iran with CNS tumors had high quality of care but availability of advanced treatment protocol and sharing of cooperative trial study is essential for survival improvement. In addition to use more advanced methods and equipments of radiotherapy and chemotherapeutic drugs, we should seriously consider development of better surgical techniques to increase the rate of complete resection of malignant CNS tumors.

Effect of washing on the bioefficacy of insecticide-treated nets (ITNs) and long-lasting insecticidal nets (LLINs) against main malaria vector Anopheles stephensi by three bioassay methods.

BACKGROUND & OBJECTIVES: The use of pyrethoid impregnated bednets is one of the main malaria vector control strategies worldwide. The objective of the present study was to evaluate the bioefficacy of bednets impregnated with various pyrethroids after repeated washings. METHODS: The effectiveness of bednets impregnated with permethrin, deltamethrin, bifenthrin, etofenprox and long-lasting bednets like OlysetNet and PermaNet which were provided by WHOPES was evaluated. The tests were carried out according to the WHO-recommended methods. Malaria vector, Anopheles stephensi was exposed to impregnated bednets for 3 min and the mortality was measured after 24 h recovery period. Knockdown was measured as well. RESULTS: Results of three methods of bioassay tests showed that between two LLINs, PermaNet was more efficient than OlysetNet. Results of ITNs exhibited that deltamethrin and permethrin were more effective than etofenprox and bifenthrin as impregnants. INTERPRETATION & CONCLUSION: Findings of this study will be useful for WHO, local authorities and people who wish to use different pyrethroid-impregnated bednets for malaria vector control.