1.
Int J Biochem
; 18(9): 813-20, 1986.
Article
in English
| MEDLINE
| ID: mdl-3758463
ABSTRACT
Leupeptin (N-acetyl-L-leucyl-L-leucyl-L-argininal) was produced as an extracellular metabolite by Streptomyces lavendulae. Addition of lysine to the growth medium stimulated leupeptin production from 200 to 1400 micrograms/ml. Incubation of late exponential phase mycelium in a synthetic medium was used to prepare single labelled (3H) and double labelled (3H/14C) inhibitor for metabolic studies. An improved purification scheme that generates leupeptin of high purity with good recoveries is also reported.
Subject(s)
Leupeptins/isolation & purification , Oligopeptides/isolation & purification , Streptomyces/growth & development , Carbon Radioisotopes , Culture Media , Kinetics , Leupeptins/biosynthesis , Tritium
2.
Prog Clin Biol Res
; 180: 269-71, 1985.
Article
in English
| MEDLINE
| ID: mdl-3898100