ABSTRACT
Plants employ metal tolerance proteins (MTPs) to confer tolerance by sequestering excess ions into vacuoles. MTPs belong to the cation diffusion facilitator (CDF) family, which facilitates the transport of divalent transition metal cations. In this study, we conducted a comprehensive analysis of the MTP gene families across 21 plant species, including maize (Zea mays). A total of 247 MTP genes were identified within these plant genomes and categorized into distinct subgroups, namely Zn-CDF, Mn-CDF, and Fe/Zn-CDF, based on phylogenetic analyses. This investigation encompassed the characterization of genomic distribution, gene structures, cis-regulatory elements, collinearity relationships, and gene ontology functions associated with MTPs. Transcriptomic analyses unveiled stress-specific expression patterns of MTP genes under various abiotic stresses. Moreover, quantitative RT-PCR assays were employed to assess maize MTP gene responses to diverse heavy metal stress conditions. Functional validation of metal tolerance roles was achieved through heterologous expression in yeast. This integrated evolutionary scrutiny of MTP families in cereals furnishes a valuable framework for the elucidation of MTP functions in subsequent studies. Notably, the prioritized MTP gene ZmMTP6 emerged as a positive regulator of plant Cd tolerance, thereby offering a pivotal genetic asset for the development of Cd-tolerant crops, particularly maize cultivars.
ABSTRACT
The present study aimed to assess the influences of corn straw application on the soil microbial organisms, soil enzyme activities and the grain yield. Four treatments were evaluated: (i) The straw was ploughed into soil using a fence hydraulic turning plow with ploughing depth of 30-40 cm(PD). (ii) The self-developed straw deep returning machine was used to bury 30-40 cm in the sub-surface layer of soil (SD). (iii) The straw was mulched and no tillage sowing(M). (iv)Without straw application(CK). Soil samples of different deep(0-20 cm, 20-40 cm soil layer) were taken during the corn growth stage to determinesoil biological characteristics.Our results suggested that soil microorganisms were not increased by straw mulching. Straw deep ploughing and returning (PD treatment) could effectively improve the phospholipid fatty acids(PLFAs) of bacteria, actinomycetes, and fungi, the activities of urease,invertase,dehydrogenase and polyphenoloxidase, even the grain yield. In 20-40 cm subsoil layer, the effects were more obvious than those of topsoil. The mean yield of PD treatment was higher than SD,M and CK. The results showed that the PLFA signatures and soil enzyme were both sensitive to the changes of soil environment condition by the application of straw. In the actual field production, we should adopt the appropriate way of straw returning to the field to achieve not only the improvement of soil quality, but also the increase of grain yield.
