[Structural transformation of human erythrocyte membranes initiated by group specific antibodies]. / Strukturnyi perekhod v membranakh éritrotsitov cheloveka, initsiiruemyi gruppospetsificheskimi antitelami.

Some changes have been found in the rates of the desintegration by sodium dodecylsulfate of human erythrocite membranes (EM) after incubation with specific group antibodies (GA) from incompatible blood. GA have no effect on the disintegration rates of EM by sodium desoxycholate and Triton X-100. The data are considered as the evidence of the structural transition in EM induced by GA and resulting in modification of integrative coupling forces primarily in the protein components of the membrane. The stopflow technique has been used to investigate the dependence of the desintegration rates in the quick phase (10 to 100 ms) upon the GA concentrations in the incubation medium and upon the incubation time. The obtained curves are distinctly S-shaped that is peculiar of cooperative processes.

[Disintegration and fractionation of barley chloroplast membranes at different concentrations of digitonin and chloroplasts]. / Dezintegratsiia i fraktsionirovanie khloroplastnykh membran iachmenia pri razlichnom soderzhanii digitonina i khloroplastov.

Chloroplasts pretreated with digitonin were fractionated by electrophoresis in order to study the arrangement of chloroplast membranes. Different types of particles resulting from the electrophoresis are shown to be due to fractionation of the chloroplast membranes of the same type rather than to the isolation of particular types from the chloroplasts of different ages. The stopped-flow measurement of quickly changing light scattering demonstrated that the membrane solubilization increased with the increase in the digitonin concentration. Meanwhile the fractionation result (the amount of particles in the electrophoretic zones and their spectral properties) remain very similar over a wide range of the detergent and chloroplast concentrations. This is explained by the fact that the increasing detergent concentrations do not cause particle destruction. Thus the method used revealed the particles which different from one another before they were isolated from the membrane. They are fractionated following the natural boundaries between them and the containing membrane. In other words, chloroplast membranes have a discrete set of submembrane particles of different types.

[Structural rearrangements in membranes induced by anesthetics]. / Strukturnye perestroiki membran, indutsiruemye anestetikami

Studies were carried out on the effects of anesthetics on the stability of membranes to sodium dodecylsulfate and trypsin. The initial rate of membrane desintegration by detergent evaluated by light scattering with stopped flow method was increased in the presence of ethanol, propanol, benzyl alcohol, procain-amide (erthrocyte membrane) and chlorpromazine (rat brain synaptosomes). Concentrations of anesthetics which correspond to half of the maximum effect were very close to that of blocking the action potential and inducing 50%--antihemolysis. Alcohols and procaine at anesthetic concentrations inhibited proteolysis of isolated erythrocyte membranes but were without effect on their ultrasonic fragments. Increase of membrane desintegtation rate is interpreted as structural rearrangements of membranes with the weakening of detergent sensitive intermolecular interactions. Proteolysis inhibition of ghosts but not the gragments indicates against the participation of the basic func of proteins in the structural membrane rearrangements.