Impact of Local Drug Delivery of Minocycline on the Subgingival Microbiota during Supportive Periodontal Therapy: A Randomized Controlled Pilot Study.

The aim of this study was to examine the effect of adjunct local minocycline administration on the microbiological parameters of subgingival plaque samples in the residual periodontal pockets. Ten chronic periodontitis patients under a supportive periodontal therapy regimen were recruited. After subgingival debridement, either 2% minocycline gel, Periocline™, (Test Group) or a placebo (Control Group) was administered to the selected sites once a week for three weeks. Subgingival plaque was collected at baseline, and at four weeks and eight weeks. The microbiological composition was analyzed by 16S ribosomal RNA sequencing. In the Test Group, α-diversity (evenness) decreased compared to the baseline (p = 0.005) and was lower compared to the control group at four weeks (p = 0.003). The microbial community composition between the two groups was significantly different at four weeks (p = 0.029). These changes were attributable to a decrease in the bacteria associated with periodontitis and an increase in the bacteria associated with periodontal health. Additionally, the improvement in bleeding on probing continued at eight weeks; however, there were little microbial effects of 2% minocycline gel observed at eight weeks. The control group demonstrated no change throughout the eight-week experimental period. Thus, local minocycline administration can change the subgingival microbial community of residual periodontal pockets.

[Application of dietary supplement to periodontal disease and osteoporosis].

It is known that there is a relationship between various systemic diseases, life style and periodontal disease. In such a situation, it is becoming clear a relationship between the systemic bone density and the mandibular bone, alveolar bone density and clinical periodontal parameters. And it is also cleared that to lower common risk factors related to these conditions might be useful to prevent osteoporosis and periodontal disease. As it is reported that medical treatment such as hormone replacement therapy (HRT) is effective not only preventing lowering skeletal bone density but also maintaining mandibular and alveolar bones, it is also considered that continuing supplementation of some nutritions such as calcium and isoflavone is also expected to have preventive effects.

[Calcium and the risk for periodontal disease].

Recently, there is much interest regarding the relationship between periodontal disease and the life style as well as systemic disease including osteoporosis. In addition, there is increasing awareness that dietary calcium intake and other nutrients are important for prevention and management of periodontal disease. In this paper, a cross-sectional study from NHANES III for investigating the association between dietary calcium intake and periodontal disease is introduced, and the management of periodontal disease is proposed from nutritional aspects.