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1.
Ann Oncol ; 24(10): 2560-2565, 2013 Oct.
Article in English | MEDLINE | ID: mdl-23884439

ABSTRACT

BACKGROUND: Since the best chemotherapy regimen for each patient with advanced gastric cancer is uncertain, we aimed to identify molecular prognostic or predictive biomarkers from biopsy specimens in JCOG9912, a randomized phase III trial for advanced gastric cancer. PATIENTS AND METHODS: Endoscopic biopsy specimens from primary lesions were collected in 445 of 704 randomized patients in JCOG9912. We measured the mRNA expression of excision repair cross-complementing group 1 (ERCC1), thymidylate synthase, dihydropyrimidine dehydrogenase, and five other genes, then, categorized them into low and high groups relative to the median, and examined whether gene expression was associated with efficacy end point. RESULTS: Multivariate analyses showed that high ERCC1 expression [HR 1.37; 95% confidence interval (CI) 1.08-1.75; P = 0.010], performance status ≥ 1 (HR 1.45; 95% CI 1.13-1.86; P = 0.004), and number of metastatic sites ≥ 2 (HR 1.66; 95% CI 1.28-1.86; P < 0.001) were associated with a poor prognosis, and recurrent disease (versus unresectable; HR 0.75; 95% CI 0.56-1.00; P = 0.049) was associated with a favorable prognosis. None of these molecular factors were a predictive marker for choosing irinotecan plus cisplatin or 5-fluorouracil rather than S-1. CONCLUSION: These correlative analyses suggest that ERCC1 is an independent prognostic factor for overall survival in the first-line treatment of gastric cancer. CLINICAL TRIAL NUMBER: C000000062, www.umin.ac.jp.


Subject(s)
DNA-Binding Proteins/metabolism , Endonucleases/metabolism , Stomach Neoplasms/drug therapy , Antimetabolites, Antineoplastic/therapeutic use , Antineoplastic Agents, Phytogenic/therapeutic use , Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Biomarkers, Tumor/biosynthesis , Biomarkers, Tumor/genetics , Biomarkers, Tumor/metabolism , Camptothecin/analogs & derivatives , Camptothecin/therapeutic use , Cisplatin/therapeutic use , DNA-Binding Proteins/genetics , Dihydrouracil Dehydrogenase (NADP)/genetics , Drug Combinations , Endonucleases/genetics , Female , Fluorouracil/therapeutic use , Gene Expression , Humans , Irinotecan , Male , Oxonic Acid/therapeutic use , Prognosis , RNA, Messenger/biosynthesis , Stomach Neoplasms/genetics , Stomach Neoplasms/mortality , Survival , Tegafur/therapeutic use , Thymidylate Synthase/genetics , Vascular Endothelial Growth Factor A/biosynthesis , Vascular Endothelial Growth Factor A/genetics
2.
J Gastroenterol ; 31(4): 498-504, 1996 Aug.
Article in English | MEDLINE | ID: mdl-8844469

ABSTRACT

Almost completely homogenous gastric mucous epithelial cells of guinea pigs were grown to confluence in the presence of 10% fetal calf serum (FCS). FCS, epidermal growth factor (EGF), and insulin significantly increased 5-bromo-2'-deoxyuridine (BrdU) uptake by the cells and EGF together with insulin increased the cells' [3H] thymidine uptake. Basic fibroblast growth factor (bFGF) enhanced EGF-induced DNA synthesis by the cells, but vasoactive intestinal peptide (VIP), secretin, prostaglandin E2 (PGE2), and dibutyryl cyclic AMP (dbcAMP) neither induced DNA synthesis nor enhanced the effect of EGF on DNA synthesis by the cells. Gastrin, cholecystokinin-octapeptide (CCK8), and carbamylcholine chloride (CCh) also did not enhance the effect of EGF on DNA synthesis. 125I-EGF, 125I-bFGF, and 125I-gastrin binding to the gastric mucous cells revealed the presence of high-affinity receptors for EGF and bFGF, but not for gastrin. Northern blot analysis showed the expression of EGF receptor mRNA, but not gastrin receptor mRNA. These results suggest that EGF, insulin, and bFGF may cooperatively regulate gastric mucous cell growth, but that gastrin and other gastrointestinal hormones do not have a direct stimulatory effect on mucous cell growth in the guinea pig.


Subject(s)
Gastric Mucosa/cytology , Gastrointestinal Hormones/pharmacology , Growth Substances/pharmacology , Animals , Blotting, Northern , Cell Division/drug effects , Cells, Cultured , ErbB Receptors/biosynthesis , ErbB Receptors/genetics , ErbB Receptors/physiology , Gastric Mucosa/drug effects , Gastrointestinal Hormones/physiology , Growth Substances/physiology , Guinea Pigs , Iodine Radioisotopes , RNA, Messenger/genetics , Receptors, Cholecystokinin/biosynthesis , Receptors, Cholecystokinin/genetics , Receptors, Cholecystokinin/physiology
3.
J Gastroenterol ; 31(3): 431-6, 1996 Jun.
Article in English | MEDLINE | ID: mdl-8726837

ABSTRACT

Choriocarcinoma, a malignant tumor of usually placental origin, in divided into two groups; the gestational and non-gestational types, the latter being rare. Non-gestational choriocarcinoma occurs in the lung, mediastinum, kidney, stomach, and small intestine, but rarely appears in the large intestine. We treated a 29-year-old woman with choriocarcinoma of the rectum with adenocarcinoma. Despite the rarity of the condition and the obscurity of the histogenesis, reports of similar cases and the occurrence of the tumors in the digestive tract suggest that the condition constitutes a clinical entity of a digestive tumor.


Subject(s)
Adenocarcinoma/genetics , Choriocarcinoma/genetics , Genes, p53/genetics , Genes, ras/genetics , Neoplasms, Multiple Primary/genetics , Rectal Neoplasms/genetics , Adenocarcinoma/pathology , Adult , Choriocarcinoma/pathology , Codon/genetics , Female , Humans , Lung Neoplasms/secondary , Mutation , Neoplasms, Multiple Primary/pathology , Rectal Neoplasms/pathology , Rectum/pathology
4.
Gan To Kagaku Ryoho ; 23(7): 923-8, 1996 Jun.
Article in Japanese | MEDLINE | ID: mdl-8678544

ABSTRACT

Three patients with chemotherapeutically pretreated metastatic gastric cancer were given MAC-VD therapy combining MMC, ADM, CDDP, Etoposide (VP-16) and 5'-DFUR. All were evaluable for their responses. Patients ranged in age from 49 to 61 years. Performance status scale (P.S.) grade 0 was two cases; and P.S. grade 1 was one case. The overall response rate, CR+PR, was 0+2/3 (66.7%). The response rate in the primary lesions was 0%, against 66.7% (2/3) in the liver, 100% (1/1) in the spleen, 100% (1/1) in the lung, and 0% in the abdominal lymph node metastasis. The chief manifestations of toxicity were hematologic, such as leukocytopenia, anemia and thrombocytopenia in 100% of the cases. Non-hematologic toxicity was seen in alopecia in 66.7%, diarrhea in 33.3%, fever in 33.3%, and pigmentation in 33.3%. Severe toxicity was not observed. From these data, the administration of MAC-VD therapy was considered tolerable and these data suggested that this therapy could be given as a second-line chemotherapy when initial treatment failed to obtain a response after a partial response.


Subject(s)
Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Liver Neoplasms/drug therapy , Liver Neoplasms/secondary , Stomach Neoplasms/drug therapy , Cisplatin/administration & dosage , Doxorubicin/administration & dosage , Etoposide/administration & dosage , Female , Floxuridine/administration & dosage , Humans , Lymphatic Metastasis , Male , Middle Aged , Mitomycin/administration & dosage , Neoplasm Recurrence, Local/drug therapy , Stomach Neoplasms/pathology
5.
Dig Dis Sci ; 40(8): 1679-86, 1995 Aug.
Article in English | MEDLINE | ID: mdl-7648967

ABSTRACT

The present study was undertaken to investigate whether epidermal growth factor (EGF) could stimulate prostaglandin E2 release, and if so, by what mechanism EGF would exert such an effect in gastric mucosal cells. In cultured guinea pig gastric mucous cells, EGF dose-dependently stimulated prostaglandin E2 release, with maximal stimulation observed at 10 ng/ml. EGF stimulated an increase in cyclooxygenase activity, which was reduced by protein synthesis inhibitor, actinomycin D, and cycloheximide. EGF also stimulated the enzyme protein synthesis estimated by Western blot analysis, whereas EGF did not stimulate phospholipase A2 activity. These results suggest that such an effect of EGF of de novo synthesis of cyclooxygenase protein and prostaglandin E2 release may be involved at least in part in the mechanism of EGF-induced local regulation of gastric mucosal integrity.


Subject(s)
Dinoprostone/metabolism , Epidermal Growth Factor/physiology , Gastric Mucosa/metabolism , Prostaglandin-Endoperoxide Synthases/biosynthesis , Animals , Cell Division/drug effects , Cells, Cultured , Cycloheximide/pharmacology , Cyclooxygenase Inhibitors/pharmacology , Dactinomycin/pharmacology , Dose-Response Relationship, Drug , Enzyme Induction , Epidermal Growth Factor/pharmacology , Gastric Mucosa/cytology , Guinea Pigs , Male , Phospholipases A/metabolism , Phospholipases A2
6.
J Gastroenterol ; 29 Suppl 7: 73-6, 1994 Jul.
Article in English | MEDLINE | ID: mdl-7921158

ABSTRACT

Epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), and insulin all dose-dependently stimulated [3H]-thymidine incorporation into guinea pig gastric mucous cells cultured in vitro. On the other hand, other growth factors, e.g., platelet-derived growth factor (PDGF) and gastrointestinal hormones, such as gastrin, had no effect on DNA synthesis in these cells. Exposure of the cells to EGF at concentrations which stimulated DNA synthesis caused increases in both prostaglandin (PG) E2 release and cyclooxygenase (COX) enzyme protein synthesis, as evaluated by Western blot analysis. These results suggest that such increases in DNA synthesis and PGE2 release may be involved, at least in part, in the mechanism of EGF-induced local regulation of gastric mucosal integrity.


Subject(s)
Epidermal Growth Factor/pharmacology , Gastric Mucosa/enzymology , Prostaglandin-Endoperoxide Synthases/metabolism , Animals , Blotting, Western , Cell Division , Cells, Cultured , DNA/biosynthesis , Dinoprostone/metabolism , Fibroblast Growth Factor 2/pharmacology , Gastric Mucosa/cytology , Gastric Mucosa/metabolism , Guinea Pigs , Insulin/pharmacology , Radioimmunoassay
7.
Gastroenterol Jpn ; 28 Suppl 5: 122-6, 1993 May.
Article in English | MEDLINE | ID: mdl-8359621

ABSTRACT

In order to clarify how PGE2 regulates gastric mucosal integrity, we examined the effects of PGE2 on ethanol-caused injury of isolated gastric chief cells, cultured gastric mucous cells from guinea pigs and Balb/c 3T3 fibroblasts. Pretreatment of these cells with PGE2 reduced ethanol-caused injury of the cells. Furthermore, pretreatment of gastric mucous cells with indomethacin enhanced ethanol-caused injury, suggesting that endogenous PGE2 may be involved in the cell protection. PGE2 stimulated an increase in diacylglycerol (DG) accumulation in chief cells and treatment of chief cells with synthetic DG reduced the injury of the cells. However, DG accumulation was not observed in gastric mucous cells treated with PGE2. Therefore PGE2 may protect the cells from injury through a variety of mechanisms. In addition, PGE2 enhanced the survival of the quiescent fibroblasts cultured in the absence of serum, while PGE2 had no survival enhancing effect on gastric mucous cells. These results suggest that the mechanism by which PGE2 preserves the cell viability may depend on not only cell types used but also how the cells are injured.


Subject(s)
Dinoprostone/physiology , Gastric Mucosa/cytology , Animals , Apoptosis/physiology , Cell Survival/drug effects , Cells, Cultured , Culture Media , Diglycerides/metabolism , Ethanol/adverse effects , Fibroblasts , Gastric Mucosa/drug effects , Guinea Pigs , In Vitro Techniques , Indomethacin/pharmacology , Mice , Mice, Inbred BALB C
8.
Nihon Shokakibyo Gakkai Zasshi ; 90(1): 9-15, 1993 Jan.
Article in Japanese | MEDLINE | ID: mdl-8433540

ABSTRACT

To understand the mechanism by which prostaglandins (PGs) preserve gastric mucosal integrity, we established a primary cultured monolayer of guinea pig gastric mucous cells, and by using this culture system, we studied whether endogenously released PGE2 could influence the proliferation of the mucous cells. By the histochemical and morphological analysis at 24h of the culture periods, the cells were recognized to contain PAS-positive mucous granules with only 3% of them being parietal cells. Although the cells which were simultaneously labeled with [3H] arachidonic acid in 0.5% serum-containing medium synthesized and released radiolabeled PGE2, PGI2 and PGA2, the release of PGE2 was more markedly observed and was partially dependent upon arachidonic acid added to the culture medium. By radioimmunoassay of the culture media, the mucous cells were found to release PGE2 in a time-dependent manner in response to 10% serum. Pretreatment of the cells with 10(-4)M indomethacin not only inhibited PGE2 release but also inhibited increase in cell number. However, the addition of PGE2 dose-dependently restored the indomethacin-induced inhibition of cell growth with the maximal increase almost to the control level at 10(-6)M PGE2. These results suggest that PGE2 endogenously released from the cells may exert a proliferative effect on gastric mucous cells.


Subject(s)
Dinoprostone/pharmacology , Gastric Mucosa/cytology , Gastric Mucosa/drug effects , Animals , Cell Division/drug effects , Cells, Cultured , Dinoprostone/physiology , Guinea Pigs , Male
9.
Dig Dis Sci ; 37(10): 1593-9, 1992 Oct.
Article in English | MEDLINE | ID: mdl-1396007

ABSTRACT

To better understand phosphatidylcholine synthesis in the stomach, we isolated guinea pig gastric glands and examined their [3H]choline incorporation into phosphatidylcholine in response to either antiulcer drugs such as geranylgeranylacetone (GGA) and H2-receptor antagonists or agents that cause phosphatidylcholine synthesis in other tissues. [3H]Choline incorporation was stimulated by GGA, palmitate, and 12-O-tetradecanoylphorbol-13-acetate (TPA). Dibutyryl cyclic-AMP had no effect. By contrast with GGA, famotidine, ranitidine, and cimetidine equipotently inhibited [3H]choline incorporation into phosphatidylcholine. GGA, palmitate, and TPA increased phosphatidyl-[3H]choline and decreased phosphoryl-[3H]choline as compared with control in tissues that had been pulsed with [3H]choline. On the other hand, no more decrease in [3H]choline incorporation at chase periods was observed in pulse-labeled glands in response to each H2-receptor antagonist. The particulate fraction of glands that had been incubated with GGA or palmitate had more CTP-phosphocholine cytidylyltransferase activity than that of glands incubated without agents. A decrease in choline kinase activity was not observed in the cytosolic fraction of glands that had been incubated with cimetidine. These results suggest that GGA and palmitate stimulate phosphatidylcholine synthesis by activating cytidylyltransferase, and H2-receptor antagonists may affect phosphatidylcholine synthesis by inhibiting choline uptake in the gastric glands.


Subject(s)
Anti-Ulcer Agents/pharmacology , Gastric Mucosa/drug effects , Phosphatidylcholines/biosynthesis , Analysis of Variance , Animals , Choline Kinase/drug effects , Cytosol/enzymology , Dose-Response Relationship, Drug , Gastric Mucosa/enzymology , Gastric Mucosa/metabolism , Guinea Pigs , In Vitro Techniques , Protein Kinases/drug effects , Tritium
10.
Gastroenterol Jpn ; 27(4): 473-81, 1992 Aug.
Article in English | MEDLINE | ID: mdl-1326464

ABSTRACT

The muscarinic receptor system involved in pepsinogen secretion from isolated guinea pig gastric chief cells was investigated by evaluating the effect of muscarinic receptor antagonists on carbamylcholine (CCh)-stimulated chief cell responses. CCh stimulated the hydrolysis of polyphosphoinositide in chief cells at the same concentrations as it stimulated pepsinogen secretion. Each of five different muscarinic receptor antagonists, atropine, pirenzepine, 4-diphenylacetoxy-N-methylpiperidine methiodide (4-DAMP), AF-DX116 and scopolamine, inhibited both pepsinogen secretion and inositol phosphate accumulation stimulated by graded concentrations of CCh. The pA2 values of the antagonists calculated from data on inositol phosphate accumulation and pepsinogen secretion (atropine = scopolamine = 4-DAMP greater than pirenzepine greater than AF-DX116) suggest that the muscarinic acetylcholine receptor in gastric chief cells is the M3 subtype. On the other hand, CCh did not affect the adenylate cyclase/cAMP signaling pathway in gastric chief cells. All pA2 values of the antagonists were also in agreement with the Ki values determined by [3H]NMS binding to chief cells. Furthermore, GTP gamma S reduced [3H]acetylcholine binding to chief cell membranes in a concentration-dependent manner. The present study, therefore, suggests that muscarinic M3 receptors, which may be coupled to a G protein, mediate pepsinogen secretion, probably by activation of the polyphosphoinositide second messenger system in guinea pig gastric chief cells.


Subject(s)
Gastric Mucosa/metabolism , Parasympatholytics/pharmacology , Pepsinogens/metabolism , Phosphatidylinositols/metabolism , Receptors, Muscarinic/physiology , Animals , Carbachol/pharmacology , Dose-Response Relationship, Drug , Gastric Mucosa/drug effects , Guinea Pigs , Hydrolysis/drug effects , Inositol Phosphates/metabolism
11.
Biochem Biophys Res Commun ; 187(1): 332-9, 1992 Aug 31.
Article in English | MEDLINE | ID: mdl-1520317

ABSTRACT

To understand the molecular mechanism of gastric tumorigenesis, the status of neurofibromatosis type 1 (NF1) gene was analyzed in human gastric cancer cell lines. Although the sequencing of the GTPase activating protein (GAP)-related region of NF1 (NF1-GRD) revealed no apparent mutation, the NF1-GRD transcript (type I) and that containing an additional 63 bp insert in the center of NF1-GRD (type II) were equally expressed in most gastric cancer cells. By contrast, type II was predominantly expressed in normal stomach mucosa. When these two types of NF1-GRD were bacterially expressed and their GAP activities were tested, both types of NF1-GRD similarly stimulated ras GTPase activity. However, arachidonic acid inhibited GAP activities of two types of NF1-GRD to different extents. These results suggest that the increased expression of type I NF1 protein may modulate ras-related signal transduction and it may be related to the control of the gastric cellular proliferation.


Subject(s)
Gene Expression , Genes, Neurofibromatosis 1/genetics , Proteins/metabolism , Stomach Neoplasms/genetics , Transcription, Genetic , Arachidonic Acid/pharmacology , Base Sequence , DNA/chemistry , DNA/genetics , GTPase-Activating Proteins , Gastric Mucosa/metabolism , Humans , Molecular Sequence Data , Polymerase Chain Reaction , Proteins/chemistry , Recombinant Fusion Proteins/metabolism , Recombinant Fusion Proteins/pharmacology , Sequence Homology, Nucleic Acid , Signal Transduction , ras GTPase-Activating Proteins
12.
Cancer Res ; 52(16): 4335-41, 1992 Aug 15.
Article in English | MEDLINE | ID: mdl-1322785

ABSTRACT

To further investigate the role of p53 gene inactivation in gastric tumorigenesis, the mutational status of the p53 gene in primary human gastric cancer samples was examined. Reverse transcriptase polymerase chain reaction and subsequent direct sequencing of the p53 gene from gastric cancer samples revealed frequent point mutations of the p53 gene: some of these coincided with those previously identified in gastric cancer cell lines. In addition, both allelic deletion analysis using pYNZ 22 and polymerase chain reaction-restriction fragment length polymorphism analysis demonstrated an allelic deletion of the p53 gene in cancer tissue which contained a point mutation of the p53 gene in the remaining allele. Transfection of the wild-type or mutant p53 genes into gastric cancer cells showed that the wild-type but none of the mutated p53 genes suppressed the colony formation of gastric cancer cells. Furthermore, the incorporation of thymidine into DNA was reduced in cancer cells expressing the wild-type p53 gene. The glutathione S-transferase-wild type p53 fusion protein bound to simian virus 40 large T antigen in COS-1 cell lysate. None of the p53 fusion proteins containing mutations at codons 143, 175, 248, or 273 bound to simian virus 40 large T antigen. By contrast, two different mutant p53 fusion proteins containing mutations specifically observed in gastric cancer bound to simian virus 40 large T antigen. These results indicate that inactivation of the p53 gene through mutations and the allelic deletion may play an important role in gastric tumorigenesis. These mutations may cause a conformational change in the p53 protein resulting in the loss of the suppression by p53 of the growth of gastric cells, partly through disruption of the association of p53 protein with a cellular component.


Subject(s)
Chromosome Deletion , Chromosomes, Human, Pair 17 , Genes, p53/genetics , Mutation/genetics , Stomach Neoplasms/genetics , Antigens, Viral, Tumor/metabolism , Base Sequence , Blotting, Southern , Cell Division/genetics , Codon/genetics , Heterozygote , Humans , Molecular Sequence Data , Plasmids/genetics , Polymerase Chain Reaction , RNA, Messenger/analysis , RNA, Neoplasm/analysis , Simian virus 40 , Stomach Neoplasms/pathology , Transfection , Tumor Suppressor Protein p53/metabolism
13.
Nihon Shokakibyo Gakkai Zasshi ; 89(3): 633-8, 1992 Mar.
Article in Japanese | MEDLINE | ID: mdl-1578808

ABSTRACT

To investigate the pathogenesis of hereditary pancreatitis we determined whether pancreatic stone protein (PSP) gene was structurally altered in two independent families diagnosed as hereditary pancreatitis. Because, it has been shown that a decrease in the activity of PSP which inhibits CaCO3 crystal formation in pancreatic juice is closely related to the development of chronic calcifying pancreatitis. Southern blot analysis revealed neither a rearrangement nor a gross deletion of PSP gene in genomic DNA of affected members of both families. Furthermore, six exons of PSP gene amplified by polymerase chain reaction from genomic DNA was directly sequenced, while no apparent base mutation was observed. The immunohistochemical study utilizing monoclonal antibody to PSP showed the presence of immunoreactive PSP in the section of pancreatic tissue obtained from a patient affected with hereditary pancreatitis. However, the level of immunoreactive PSP in the remaining acinar cells of the patient pancreas was not reduced when compared with that of normal pancreas. Results, therefore, indicate that genetic alteration of PSP gene may not be responsible for the pathogenesis of hereditary pancreatitis.


Subject(s)
Calcium-Binding Proteins/genetics , Genes , Nerve Tissue Proteins , Pancreatitis/genetics , Adolescent , Adult , Base Sequence , Calcium-Binding Proteins/metabolism , Child, Preschool , DNA/analysis , Family Health , Female , Humans , Immunohistochemistry , Lithostathine , Male , Molecular Sequence Data , Pancreas/metabolism , Polymerase Chain Reaction
14.
Nihon Shokakibyo Gakkai Zasshi ; 89(2): 517-21, 1992 Feb.
Article in Japanese | MEDLINE | ID: mdl-1569706

ABSTRACT

To better understand the mechanism underlying the inhibition induced by cholecystokinin (CCK) of phosphatidylcholine (PC) synthesis, the effects of CCK treatment on the activities of enzyme involved in PC synthesis via CDP-choline pathway were studied in isolated rat pancreatic acini. CCK treatment of acini reduced CTP: phosphocholine cytidylyltransferase activity in both cytosolic and particulate fraction. However, CCK treatment of acini did not alter the activities of choline kinase and phosphocholinetransferase in acini. When acini were labeled with [3H] myristic acid and chased, CCK8 (1 nM) reduced the synthesis of [3H] myristic acid labeled-PC to 27% of control after 60 min-chase period. This inhibition of PC synthesis induced by CCK was accompanied by a delayed disappearance of [3H] diacylglycerol (DAG), the radioactivity of which was 225% of control at 60 min. CCK also induced an increase in [3H] triacylglycerol and [3H] phosphatidic acid in acini. These results suggest that CCK inhibits PC synthesis by inducing the inhibition of CTP: phosphocholine cytidylyltransferase activity. The inhibition by CCK of PC synthesis may contribute to the sustained accumulation of DAG in pancreatic acinar cells.


Subject(s)
Cholecystokinin/pharmacology , Pancreas/drug effects , Phosphatidylcholines/biosynthesis , Animals , Cells, Cultured , Depression, Chemical , Diglycerides/metabolism , Male , Pancreas/metabolism , Rats , Rats, Inbred Strains
15.
Biochem Biophys Res Commun ; 182(1): 215-23, 1992 Jan 15.
Article in English | MEDLINE | ID: mdl-1370612

ABSTRACT

To investigate the molecular pathogenesis of human gastric cancers the p53 gene, a suppressor oncogene, was analyzed in 12 human gastric cell lines. Southern blot and Northern blot analysis revealed a total deletion of p53 gene in KATO-III cells but no major abnormality of p53 gene in other cell lines. By the use of the reverse-transcriptase polymerase chain reaction and direct sequencing 7 cell lines showed point mutations of p53 gene resulting in amino-acid substitutions. Most of them were rare mutations which had not been observed in other types of cancers. One of these mutations was also detected through the use of PCR and oligomer-specific hybridization. Six out of 7 cell lines with mutations of p53 gene also lost one allele of chromosome 17p. Immunoblotting of cell lysates with an antibody specific to p53 demonstrated the absence of p53 protein in KATO-III cell. By contrast, the high levels of the p53 protein were observed in 5 cell lines all of which contained mutations of p53 gene. These results further suggest that the inactivation of p53 gene may play an important role in the transformation of gastric cells to the malignant phenotype. KATO-III cells might be a good model for studying the significance of the loss of p53 gene in cellular transformation.


Subject(s)
Chromosome Deletion , Genes, p53 , Mutation , Stomach Neoplasms/genetics , Alleles , Amino Acid Sequence , Base Sequence , Blotting, Northern , Blotting, Southern , Cell Line , Codon/genetics , DNA, Neoplasm/genetics , DNA, Neoplasm/isolation & purification , Humans , Molecular Sequence Data , Oligodeoxyribonucleotides , Polymerase Chain Reaction/methods , RNA, Neoplasm/genetics , RNA, Neoplasm/isolation & purification , RNA-Directed DNA Polymerase
16.
J Biol Chem ; 266(33): 22246-53, 1991 Nov 25.
Article in English | MEDLINE | ID: mdl-1657994

ABSTRACT

The effects of cholecystokinin (CCK) and other pancreatic secretagogues on phosphatidylcholine (PC) synthesis were studied in isolated rat pancreatic acini. When acini were incubated with [3H]choline in the presence of 1 nM CCK-octapeptide (CCK8) for 60 min, the incorporations of [3H]choline into both water-soluble choline metabolites and PC in acini were reduced by CCK8 to 74 and 41% of control, respectively. Pulse-chase study revealed that CCK8 reduced both the disappearance of phosphocholine and the synthesis of PC. Other Ca(2+)-mobilizing secretagogues such as carbamylcholine, bombesin, and Ca2+ ionophore A23187 also reduced PC synthesis to the same extent as did CCK8. When combined with 1 nM CCK8, A23187 or carbamylcholine did not further inhibit PC synthesis. Furthermore, W-7 or W-5, a calmodulin antagonist, reversed the inhibition by CCK8 of PC synthesis, suggesting that a Ca(2+)-calmodulin-dependent pathway may be involved in CCK-induced inhibition of PC synthesis in acini. By contrast, neither cAMP-dependent secretagogues such as secretin and dibutyryl cAMP nor a phorbol ester had any effect on PC synthesis in acini. Staurosporine or H-7, a protein kinase C inhibitor, did not affect the inhibition by CCK of PC synthesis. The analysis of enzyme activity involved in PC synthesis via CDP-choline pathway showed that CCK treatment of acini reduced CTP:phosphocholine cytidylyltransferase activity in both cytosolic and particulate fraction, a finding consistent with the delayed disappearance of phosphocholine induced by CCK in pulse-chase study. By contrast, CCK treatment of acini did not alter the activities of choline kinase and phosphocholine transferase in acini. The extent of inhibition by CCK of cytidylyltransferase activity became much larger when subcellular fractions of acini were prepared in the presence of phosphatase inhibitors. In addition, W-7 reversed the inhibitory effect of CCK treatment on cytidylyltransferase activity in acini. When acini were labeled with [3H]myristic acid and chased, CCK8 (1 nM) reduced the synthesis of [3H]myristic acid-labeled PC to 27% of control after a 60-min chase period. This inhibition of PC synthesis induced by CCK was accompanied by a delayed disappearance of [3H]diacylglycerol, the radioactivity of which was 225% of control at 60 min. These results indicate that CCK inhibits PC synthesis by inducing both the reduction of choline uptake into acini and the inhibition of CTP:phosphocholine cytidylyltransferase activity. Furthermore, the results suggest the possibility that the activation of Ca(2+)-calmodulin-dependent kinase in response to CCK may phosphorylate cytidylyltransferase thereby decreasing this enzyme activity in pancreatic acinar cells.(ABSTRACT TRUNCATED AT 400 WORDS)


Subject(s)
Calcium/physiology , Calmodulin/physiology , Diglycerides/metabolism , Pancreas/metabolism , Phosphatidylcholines/biosynthesis , Sincalide/pharmacology , Alkaloids/pharmacology , Animals , Bombesin/pharmacology , Bucladesine/pharmacology , Calcimycin/pharmacology , Calmodulin/antagonists & inhibitors , Carbachol/pharmacology , Choline/metabolism , Choline Kinase/metabolism , Choline-Phosphate Cytidylyltransferase , In Vitro Techniques , Kinetics , Male , Nucleotidyltransferases/metabolism , Pancreas/drug effects , Phosphatidylcholines/antagonists & inhibitors , Protein Kinase C/antagonists & inhibitors , Rats , Rats, Inbred Strains , Secretin/pharmacology , Staurosporine , Sulfonamides/pharmacology , Tetradecanoylphorbol Acetate/pharmacology , Tritium
17.
Nihon Shokakibyo Gakkai Zasshi ; 88(7): 1442-6, 1991 Jul.
Article in Japanese | MEDLINE | ID: mdl-1920900

ABSTRACT

The effects of cholecystokinin (CCK) and other pancreatic secretagogues on phosphatidylcholine (PC) synthesis were studied in isolated rat pancreatic acini. When acini were incubated with [3H] choline in the presence of 1 nM CCK-octapeptide (CCK8) for 60 min, the incorporations of [3H] choline to both water soluble choline metabolites and PC in acini were reduced by CCK8 to 74% and 41% of control, respectively. Pulse-chase study revealed that CCK reduced both the disappearance of phosphocholine and the synthesis of PC. Ca(2+)-mobilizing secretagogues such as carbamylcholine and Ca2+ ionophore A23187 also reduced PC synthesis to the same extent as CCK8. By contrast, neither cAMP-dependent secretagogues such as secretin and dibutyryl cAMP nor a phorbol ester had any effect on PC synthesis in acini. These results suggest that CCK inhibits PC synthesis by inducing both the reduction of choline uptake into acini and the inhibition of CTP: phosphocholine cytidylyltransferase activity. This hormonal regulation of PC synthesis via CDP-choline pathway appears to be mediated by Ca(2+)-dependent pathway but not by cAMP- or protein kinase C-dependent pathway.


Subject(s)
Cholecystokinin/pharmacology , Pancreas/drug effects , Phosphatidylcholines/biosynthesis , Animals , Depression, Chemical , In Vitro Techniques , Pancreas/metabolism , Rats , Rats, Inbred Strains
19.
Nihon Shokakibyo Gakkai Zasshi ; 88(6): 1281-7, 1991 Jun.
Article in Japanese | MEDLINE | ID: mdl-1653863

ABSTRACT

The mechanism by which PGE2 directly protects individual gastric cells from ethanol-induced injury was studied by using isolated gastric chief cells from guinea pig. Ethanol dose-dependently caused chief cell injury which was estimated by the release of lactate dehydrogenase (LDH) from chief cells. Pretreatment of chief cells with PGE2 reduced the cell damage caused by ethanol in time- and dose-dependent manner. The pretreatment at 37 degrees C and pH 7.4 with PGE2 maximally reduced the cell damage. This protective effect was reduced when the pretreatment was performed at either acid or alkaline pH or at reduced temperature. PGE2 did not stimulate any increase in cytosolic free Ca2+ concentration and initial Ca2+ influx rate. On the other hand, PGE2 stimulated an increase of cAMP accumulation in chief cells. However, pretreatment of chief cells with secretion, VIP, dbcAMP or forskolin failed to reduce subsequent injury caused by ethanol. These results suggest that PGE2 may protect chief cells against ethanol-caused injury probably via PGE type receptors coupled to as yet unidentified signal in gastric chief cells.


Subject(s)
Dinoprostone/pharmacology , Gastric Mucosa/cytology , Animals , Cells, Cultured , Cyclic AMP/metabolism , Epithelial Cells , Epithelium/drug effects , Ethanol , Gastric Mucosa/drug effects , Gastric Mucosa/metabolism , Guinea Pigs , L-Lactate Dehydrogenase/metabolism , Male , Sincalide/pharmacology
20.
Nihon Naibunpi Gakkai Zasshi ; 67(3): 214-21, 1991 Mar 20.
Article in Japanese | MEDLINE | ID: mdl-2070890

ABSTRACT

In order to further investigate the precise mechanisms of cholecystokinin(CCK)-induced pepsinogen secretion from gastric chief cells, we compared the signal transducing mechanisms activated by CCK with those activated by sodium fluoride (NaF) in isolated guinea pig gastric chief cells. NaF stimulated a monophasic increase in diacylglycerol accumulation with a peak value observed at 15 sec, while CCK strongly stimulated its biphasic accumulation. NaF evoked an increase in initial Ca2+ influx rate with a slow and smooth increase in intracellular free Ca2+ concentration [( Ca2+]i) monitored by fura-2, while CCK stimulated a rapid increase in [Ca2+]i followed by a late sustained phase of [Ca2+]i increase. Lanthanum chloride (La3+) effectively (unlike either nifedipine or verapamil) blocked NaF-stimulated increase in [Ca2+], but it blocked only CCK-stimulated late sustained phase of [Ca2+]i increase. La3+ reduced NaF-or CCK-stimulated maximal pepsinogen secretion to 57.0 +/- 2.5% and 73.1 +/- 3.1% of control, respectively. These results suggest that NaF activates a signal transducing mechanism which seems to be distinct from that activated by CCK, thereby inducing an increase in diacylglycerol accumulation, Ca3+ influx and pepsinogen secretion in guinea pig gastric chief cells.


Subject(s)
Cholecystokinin/pharmacology , Gastric Mucosa/cytology , Pepsinogens/metabolism , Sodium Fluoride/pharmacology , Animals , Calcium/metabolism , Cell Separation , Cells, Cultured , Diglycerides/biosynthesis , Gastric Mucosa/metabolism , Guinea Pigs , Male
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