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Int J Cancer ; 91(4): 492-6, 2001 Feb 15.
Article in English | MEDLINE | ID: mdl-11251971

ABSTRACT

To reveal genes relevant for malignant mesothelioma (MM), we carried out cDNA array experiments on 4 MM cell lines and 2 primary mesothelial cell cultures established from pleural fluid of non-cancer patients. Human cancer gene filters including 588 genes were used for the cDNA array experiments. Our study revealed 26 over-expressed genes that play a role in the regulation of cell fate, cell cycle, cell growth and DNA damage repair and 13 under-expressed genes encoding growth factors, receptors and proteins involved in cell adhesion, motility and invasion to be common to 3 or 4 MM cell lines. We confirmed the cDNA array results using RT-PCR for 5 of the over-expressed genes and for 3 of the under-expressed genes. Our study presents gene expression profiles in MM cell lines and shows the involvement of several genes, such as those encoding JAGGED1, ser/thr protein kinase NIK, Ku80 and cyclin D2, novel in MM.


Subject(s)
DNA, Complementary/metabolism , Gene Expression Regulation, Neoplastic , Mesothelioma/genetics , Mesothelioma/metabolism , Oligonucleotide Array Sequence Analysis , Down-Regulation , Electrophoresis, Agar Gel , Epithelium/metabolism , Humans , Nucleic Acid Hybridization , Reverse Transcriptase Polymerase Chain Reaction , Tumor Cells, Cultured , Up-Regulation
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