Factors affecting attitudes towards mother-to-child transmission of HIV among pregnant women in a maternal and child hospital in Thailand.

This study determines the factors which correlate with attitudes towards mother-to-child transmission of HIV in pregnant women. Using a structured questionnaire, 527 pregnant women who visited a hospital to have prenatal checkups were interviewed. The survey items were: sociodemographic characteristics, experiences of pre-test counselling, knowledge of mother-to-child transmission, and attitude towards termination of pregnancy. Results showed that many pregnant women (80%) did not have proper knowledge of the possibility of mother-to-child transmission. Logistic regression analysis also indicates that age and knowledge of the possibility of mother-to-child transmission were the significant determinants of attitudes towards termination of pregnancy. Older women who believe that all the babies of pregnant women with HIV will be infected are most likely to terminate their pregnancy when they are diagnosed as HIV positive. Considering the importance of informed decisions regarding pregnancy, this study must have important implications for future support programmes for HIV-positive pregnant women.

Glycoalkaloids in potato tubers grown under controlled environments.

Tuber content of alpha-solanine, alpha-chaconine, and total glycoalkaloids (TGA) was determined for the potato cultivars, Norland, Russet Burbank, and Denali grown under different environmental conditions in growth chambers. The lowest TGA concentrations (0.30 to 0.35 mg g-1 dry tissue) were found in the cv. Norland with 400 micromoles m-2 s-1 photosynthetic photon flux (PPF), 12 h day length, 16 C temperature, and 350 micromoles mol-1 carbon dioxide. The ratio of alpha-chaconine to alpha-solanine was close to 60:40 under all growing conditions, except that it was 50:50 under the low temperature of 12 C. Cultivars responded similarly to environmental conditions although TGA was about 20% greater in cv. Russet Burbank and about 30% greater in Denali compared to Norland. The largest changes in TGA occurred with changes in temperature. In comparison to 16 C, TGA were 40% greater at 12 C, 80% greater at 20 C, and 125% greater at 24 C (0.70 mg g-1 dry weight). The TGA concentration increased from 10 to 25% with an increase in light from 400 to 800 micromoles m-2 s-1 PPF for all three cultivars. TGA increased 20% with extension of the day length from 12 to 24 hr and also increased 20% when carbon dioxide was increased from 350 to 1000 micromoles mol-1. TGA concentrations were not influenced by changes in relative humidity from 50 to 80%. TGA concentrations decreased only slightly in harvests made from 9 to 21 weeks after planting. Variations in TGA among the different growing conditions and cultivars were below 20 mg/100 g fresh weight (approximately 1.0 mg g-1 dry weight) recognized as the upper concentration for food safety. However the results suggest that TGA should be considered when potatoes are grown at temperatures above 20 C.