Utility of an in-house real-time PCR in whole blood samples as a minimally invasive method for early and accurate diagnosis of invasive mould infections.

INTRODUCTION: Invasive mould infections (IMIs) are a leading cause of death in patients with compromised immune systems. Proven invasive mould infection requires detection of a fungus by histopathological analysis of a biopsied specimen, sterile culture, or fungal DNA amplification by PCR in tissue. However, the clinical performance of a PCR assay on blood samples taken from patients suspected of invasive mould disease has not been fully evaluated, particularly for the differential diagnosis of invasive aspergillosis (IA) and invasive Mucormycosis (IM). OBJECTIVES: To assess the diagnostic utility of our previously validated in-house real-time PCR in blood samples for diagnosis of invasive aspergillosis and mucormycosis in patients with suspected invasive mould infection. METHODS: All patients with suspected invasive mould infection were prospectively enrolled from May 2021 to July 2021. Conventional fungal diagnosis was performed using tissue and respiratory samples. In-house PCR was performed on blood samples and its diagnostic performance evaluated. RESULTS: A total of 158 cases of suspected invasive mould infection were enrolled in the study. The sensitivity and specificity of in-house PCR performed on blood samples was found to be 92.5% and 81.4% respectively for diagnosis of probable IA, and 65% and 84.62% respectively for diagnosis of proven and probable IM. It was also able to detect 3 out of 5 cases of possible IM where no other microbiological evidence of IM was obtained. CONCLUSIONS: This assay could be helpful in minimally invasive diagnosis of IMIs for patients in whom invasive sampling is not feasible, especially as a preliminary or screening test. It can help in early diagnosis, anticipating conventional laboratory confirmation by days or weeks. Possible correlation between fungal load and mortality can help in initiating aggressive treatment for patients with high initial fungal load.

A Quality Improvement (QI) initiative in diagnostic stewardship of urine culture in an era of Laboratory Information Systems (LIS).

Standard urine culture is the gold standard for diagnosing urinary tract infections (UTIs) but fails to differentiate true UTI from asymptomatic bacteriuria, which is important to prevent the overuse of antibiotics. Correlation with the presence or absence of pyuria can be helpful in giving a hint of the true situation. With the help of Laboratory Information System (LIS), patients' urinalysis reports can be conveniently accessed and compared simultaneously with appropriate reports. In our study, a quality improvement initiative was planned for appropriate reporting of urine culture and antimicrobial susceptibility testing using information obtained through LIS.

The Gram stain: Implication in blood culture reporting.

Bloodstream infections are life-threatening. They are responsible for prolonged hospital stays and high healthcare costs. Clinical microbiology has an essential role to play in its management. Direct Gram-stain reporting from positive blood culture bottles has been found helpful. We aimed to evaluate the use of the automated blood culture system in adjunct to the conventional Gram-stain technique, with a direct antimicrobial susceptibility test on the second day. Concordance was highly satisfactory.

Polymicrobial growth in standard urine culture: Time to Act or Ignore?

Diagnosing Urinary Tract Infections (UTI) remains a clinical challenge. A monobacterial growth in standard urine culture is diagnostic while polymicrobial growth is usually considered as contaminants. However, in certain cases, true mixed infections may exist making it important to correctly identify the pathogens and streamline the therapy. Communication between the clinician and microbiologist is essential to establish the right diagnosis at right time. Here, we would like to share our experience about a polymicrobial UTI in a paediatric patient in which coordinated discussion between the clinical team and microbiologist was helpful for favourable outcome.

Online classes versus traditional classes? Comparison during COVID-19.

BACKGROUND: Nowadays, the use of Internet with e-learning resources anytime and anywhere leads to interaction possibilities among teachers and students from different parts of the world. It is becoming increasingly pertinent that we exploit the Internet technologies to achieve the most benefits in the education. MATERIALS AND METHODS: This study compares the difference between traditional classroom and e-learning in the educational environment. Medical undergraduate students of our institution were enrolled to compare between the online versus traditional method of teaching through questionnaire. RESULTS: Forty percent of students found the online lecture material difficult to understand. 42.6% of respondents found it difficult to clear the doubts in online teaching; 64.4% of the participants believed that they have learned more in a face-to-face learning. CONCLUSION: In this study, we concluded that online mode offers flexibility on timing and delivery. Students can even download the content, notes, and assignment. Despite all the advantages offered, there is a general consensus that no technology can replace face-to-face teaching in real because in this, there will be visual as well as verbal discussion. Looking at the uncertainty of the current scenario, it is difficult to predict how long online classes will have to continue. Hence, it is of paramount importance that we assess the effectiveness of online classes and consequently take measures to ensure proper delivery of content to students, especially in a skilled field like medicine, so we concluded that face-to-face learning is of utmost importance in medical institutions.