Hyperbaric oxygen inhibits the HMGB1/RAGE signaling pathway by upregulating Mir-107 expression in human osteoarthritic chondrocytes.

OBJECTIVE: MicroRNA (miRNA)107 expression is downregulated but high mobility group box 1 (HMGB-1), Toll-like receptors (TLRs), and receptor for advanced glycation end products (RAGE) are upregulated in osteoarthritic (OA) cartilage. We investigated mir-107/HMGB-1 signaling in OA after hyperbaric oxygen (HBO) treatment. DESIGN: MiR-107 mimic was transfected and the HMGB-1 was analyzed in OA chondrocytes. MiRNA targets were identified using bioinformatics and a luciferase reporter assay. After HBO treatment, the mRNA or protein levels of HMGB-1, RAGE, TLR2, TLR4, and inducible nitric oxide (NO) synthase (iNOS) and phosphorylation of mitogen-activated protein kinase (MAPK) were evaluated. The secreted HMGB-1 and matrix metalloproteases (MMPs) levels were quantified. Finally, we detected the HMGB-1 and iNOS expression in rabbit cartilage defects. RESULTS: Overexpression of miR-107 suppressed HMGB-1 expression in OA chondrocytes. The 3'UTR of HMGB-1 mRNA contained a 'seed-matched-sequence' for miR-107. MiR-107 was induced by HBO and a marked suppression of HMGB-1 was observed simultaneously in OA chondrocytes. Knockdown of miR-107 upregulated HMGB-1 expression in hyperoxic cells. HBO downregulated the mRNA and protein expression of HMGB-1, RAGE, TLR2, TLR4, and iNOS, and the secretion of HMGB-1. HBO decreased the nuclear translocation of nuclear factor (NF)-κB, downregulated the phosphorylation of MAPK, and significantly decreased the secretion of MMPs. Morphological and immunohistochemical observation demonstrated that HBO markedly enhanced cartilage repair and the area stained positive for HMGB-1 and iNOS tended to be lower in the HBO group. CONCLUSIONS: HBO inhibits HMGB-1/RAGE signaling related pathways by upregulating miR-107 expression in human OA chondrocytes.

Mixed culture models for predicting intestinal microbial interactions between Escherichia coli and Lactobacillus in the presence of probiotic Bacillus subtilis.

Bacillus has been proposed as a probiotic due to its in vivo effectiveness in the gastrointestinal tract through antimicrobial activities. The present study investigates the effects of Lactobacillus alone or in the presence of Bacillus subtilis MA139 on the inhibition of pathogenic Escherichia coli K88. Mixed cultures were used to predict the possible interactions among these bacteria within the intestinal tract of animals. B. subtilis MA139 was first assayed for its inhibition against E. coli K88 both under shaking and static culture conditions. A co-culture assay was employed under static conditions to test the inhibitory effects of Lactobacillus reuteri on E. coli K88, with or without addition of B. subtilis MA139. The results showed that B. subtilis MA139 had marked inhibition against E. coli K88 under shaking conditions and weak inhibition under static conditions. Lactobacillus alone as well as in combination with B. subtilis MA139 spores exerted strong inhibition against E. coli K88 under static conditions. However, the inhibition by Lactobacillus in combination with B. subilis spores was much higher than that by Lactobacillus alone (P<0.01). B. subtilis MA139 significantly decreased the pH and oxidation-reduction potential values of the co-culture broth compared to that of Lactobacillus alone (P<0.05). The viability of Lactobacillus increased when co-cultured with B. subtilis MA139 because of significantly higher Lactobacillus counts and lower pH values in the broth (P<0.05). The role of Bacillus in the mixed culture models suggests that Bacillus may produce beneficial effects by increasing the viability of lactobacilli and subsequently inhibiting the growth of pathogenic E. coli. Therefore, the combination of Bacillus and Lactobacillus species as a probiotic is recommended.

Acute paraplegia due to thoracic extramedullary hematopoiesis in chronic myeloproliferative disorder--an unusual presentation.

Spinal cord compression due to extramedullary hematopoiesis is a well-known complication in a number of hematological diseases. Most of the patients present with progressive paraparesis due to the slow expansion of the extramedullary hematopoietic tissue. The authors report a case of chronic myeloproliferative disorder with spinal extramedullary hematopoiesis presenting with acute paraplegia. Chronic myeloproliferative disorder is an uncommon cause of spinal cord compression, especially when associated with acute paraplegia. The authors discuss the pathomechanism of this unusual presentation.

Surgical treatment of adjacent instability after lumbar spine fusion.

STUDY DESIGN: This study is a retrospective review of 39 patients with previous instrumented lumbar fusion who underwent secondary spine surgery for lumbar adjacent instability. To the authors' knowledge, this is the largest study of surgical treatment of lumbar adjacent instability in the literature to date. OBJECT: This study evaluated the feasibility of adjacent instability treated with medial facetectomy, fusion with autologous bone grafting, and pedicle screw instrumentation. SUMMARY OF BACKGROUND DATA: The surgical treatment of adjacent instability has seldom been discussed. Revision spine fusions are challenged by high pseudarthrosis rates. METHODS: Thirty-nine patients with previous lumbar fusion underwent second lumbar spine surgery for adjacent instability. All were treated with autogenous posterolateral arthrodesis and transpedicle screw fixation in addition to decompressive laminectomy. Medical records, radiographs, and pain scores were obtained. RESULTS: The clinical results were excellent or good in 76.9% of patients, and the radiographic fusion was successful in 37 (94.9%) of patients. Flat back was noted in 8 (20.5%) of patients. In 5 patients (12.8%), neighboring segment breakdown again developed, and 2 of those patients underwent a third lumbar fusion. Dural tear during operation occurred in 2 patients. One patient experienced cauda equina syndrome but recovered bladder function 1 month later. CONCLUSION: Autogenous posterolateral arthrodesis combined with pedicle screw fixation led to successful radiologic and clinical outcome in patients with lumbar adjacent instability. Adequate decompression of the adjacent stenosis requires medial facetectomy, thus preventing aggressive nerve root manipulation and reducing the incidence of dural tear.

Destructive spondyloarthropathy mimicking spondylitis in long-term hemodialysis patients.

A 63-year-old man with end-stage renal disease (ESRD) who had been undergoing hemodialysis for 18 years suffered persistent neck pain, progressive quadriparesis, and a deteriorating ataxic gait during the 6 months before admission. A sudden onset of aggravating quadriparesis and an inability to ambulate occurred during his trip to Sydney, Australia, 1 week before this admission. Vertebral tuberculosis osteomyelitis of the C5/6 segment was considered and treated in a hospital there. Findings from cervical magnetic resonance imaging (MRI; low signal intensity on both T1- and T2-weighted images) were diagnostic of destructive spondyloarthropathy (DSA) and distinguishable from spinal osteomyelitis preoperatively. Amyloid masses, mainly composed of B-2 microglobulin, filled in disc and paradiscal ligaments, with adjacent end-plate destruction by cytokine-mediated reactive inflammation, and appeared to be mostly related to the pathogenesis of DSA. The cervical spine, especially C5/6, is the most common site of DSA. Spinal instability and neurologic compression cause the clinical symptoms and signs. Adequate decompression and successful cervical fusion ensure the best therapeutic results.

Anterior reconstructive spinal surgery with Zielke instrumentation for metastatic malignancies of the spine.

From March 1984 to April 1996, 60 consecutive patients with spinal metastasis underwent palliative surgery by anterior corpectomy and Zielke instrumentation. Their ages ranged from 21 to 76 years (mean 54 years). Thirty-two patients had metastasis to the thoracic spine, 20 to the lumbar spine, and 8 had both thoracic and lumbar metastases. The primary malignancies were lung cancer in 12 patients, colorectal cancer in 10, hepatoma in 9, thyroid cancer in 7, breast cancer in 3, and cancers of the stomach, kidney, nasopharynx, long bones, skin, and cervix in 1 patient each. A primary carcinoma was never identified in 13 patients. In the present series, 4 patients died within 1 month, and 56 patients were followed-up. All maintained spinal stability postoperatively. Forty of 52 patients with severe pain obtained significant symptomatic relief for 3 months or more, and 33 of the 46 paralyzed patients gained neural improvement. Sphincter dysfunction became better in 10 patients, and none became worse. We conclude that anterior corpectomy to decompress neural encroachment with instrumental reconstruction to stabilize the collapsed spine is a good adjunctive treatment in these highly selected patients.

Acute postoperative aggravation of radiculopathy as a complication of free fat transplantation in lumbar disc surgery: case report.

This case report illustrates a rare case of motor weakness caused by a free fat graft herniation. A 40-year-old woman who had undergone surgery for a herniated lumbar intervertebral disc experienced right lower leg weakness. On magnetic resonance image (MRI) a herniated free fat graft was noted. An emergent operation was performed and the herniated fat graft was removed. Postoperatively, the patient recovered well with improvement of the motor weakness. MRI is a good method for diagnosis of fat graft herniation. The mechanisms of this complication have been documented, and the size of the fat graft plays an important role. The methods for prevention of this herniation are also discussed. Although the transplantation of adipose tissue has many advantages, including the prevention of postoperative epidural fibrosis, great care is needed when applying a fat graft intra-operatively. When a postoperative neurologic deficit develops, herniation of the fat graft must be considered. An emergent operation is the treatment of choice for this particular complication.

Simultaneous anterior dislocation of the shoulder and fracture of the ipsilateral humeral shaft. Two case reports.

Two patients with anterior dislocation of the shoulder and ipsilateral fracture of the shaft of the humerus have been studied and the mechanism of their injuries has been documented. Closed reduction of the dislocations was performed under anaesthesia during surgery for humeral fixation with a dynamic compression plate. The fractures and dislocations healed without any problems at 6 to 9 months postoperatively. At recent follow-up, one patient had returned to work and regained normal mobility. The other patient had the sequelae of a brachial plexus injury. The literature on this subject is reviewed.

Spinal fusion and pedicle screw instrumentation in the treatment of spondylolisthesis over the age of 60.

BACKGROUND: Spinal fusion or instrumentation for the treatment of spondylolisthesis in elderly people is still an issue of controversy. The purpose of this study was to assess the clinical results of laminectomy, posterolateral fusion, and pedicle screw instrumentation in patients over age 60. MATERIALS AND METHODS: From 1993 through 1994, 94 spondylolisthesis patients over age 60 underwent laminectomy, posterolateral fusion and pedicle screw instrumentation. All the patients had follow-up examinations 3 months, 6 months, 1 year and then annually after the operation. At each follow-up visit, the clinical results and complications were evaluated and a radiographic assessment was performed. The follow-up period ranged from 2 to 4 years. At the final follow-up visit, we administered a questionnaire designed for clinical evaluation. RESULTS: Seventy-five patients (80%) obtained improvement in back pain, 75 patients (80%) got improvement in leg pain, and 65 patients (69%) needed no medications. The average distance the patients were able to walk, at one time was 2.2 km. Sixty-seven patients (71%) could walk more than 500 m at a time. Seventy-four patients (79%) showed solid fusion, 20 patients inadequate fusion, and no psuedoarthrosis was found. Complications were rare. CONCLUSION: Laminectomy with spinal fusion and instrumentation is a good method for the treatment of spondylolisthesis in elderly people, and it can achieve a satisfactory clinical outcome.

Survivorship analysis of DKS instrumentation in the treatment of spondylolisthesis.

This retrospective study analyzed the survivorship of DKS instrumentation and the clinical outcomes in 185 patients with spondylolisthesis. These patients were treated with Zielke DKS instrumentation for a mean followup period of 3.5 years. Eight (4.3%) patients had late removal of implants, 25 (14%) had rod breakage, three (1.7%) had screw breakage, and 16 (8.7%) had nut loosening. The survivor rate of DKS instrumentation was 96% within 3 months after operation, 80% at 2 years, and 61% at 5 years after surgery. One hundred sixty-three (88%) patients had solid posterolateral fusion, and 167 (90%) patients had good to excellent results. Adjacent instability developed in 18 (9.7%) patients. Although Zielke DKS instrumentation has a smaller rod and relatively insecure locking system between the rod and screw, it is an effective implant for the treatment of spondylolisthesis.

[Metastatic spine disease: surgical treatment by anterior corpectomy and Ziekel's VDS fixation].

The spine is the common site of skeletal metastases. The goal of surgical treatment of metastatic spine disease is to improve the quality of remaining life, by relief of pain, the preservation or restoration of neurologic function, and reconstruction of the spinal stability. From 1984 through June 1993, 40 consecutive patients underwent surgical treatment by anterior corpectomy, and bone cement with VDS fixation for corporal metastatic diseases. There were 28 men and 12 women. The average age was 53.2 years, range from 21 to 76 years. Twenty-four patients had metastasis to the thoracic spine, 12 to the lumbar spine, and 4 had both thoracic and lumbar metastases. The primary malignancies were lung cancers in 10 cases, colorectal carcinomata in 6, hepatomas in 5, thyroid cancers in 4, cancer of stomach, renal, breast, nasopharynx, long bone, skin, cervical in 1 case respectively. A primary carcinoma was never identified in 8 patients. In the present series, 4 patients died within one month, 36 patients were allowed for follow-up study. Thirty-two patients (89%) attained pain relief. Thirty patients presented with neurologic compromise. After operation, neurologic improvement was noted in 21 (70%). No patient's neurologic function deteriorated secondary to surgical intervention. We conclude that anterior corpectomy to decompress neural encroachment with VDS and cement fixation to stabilize the collapsed spine is an adjunctive treatment to reduce pain and restore the neurologic function in these highly selected patients.

Reduction-fixation spinal system in spondylolisthesis.

Posterior transpedicular instrumentation with short segmental fixation represents a current trend in surgery for unstable spinal problems, such as spondylolisthesis. A reduction-fixation spinal system with 5.75- to 6.25-mm diameter pedicle screws of variable angles and an 8-mm diameter rod can achieve a rather rigid, short-segment fixation and satisfactory reduction of spondylolisthetic deformities. In clinical applications, 84% of 85 patients with spinal instability had good-to-excellent results for back pain, 88% for sciatica, and 86% for claudication; 91% achieved solid posterolateral fusion from the roentgenographic studies. This reduction-fixation system achieved a reduction of 71% +/- 5.3% of slipping width in this population. Posterior lumbar interbody fusion is mandatory for effective and lasting reduction. Complications from reduction fixation including pedicle-screw loosening (12%), pedicle screw breakage (7%), adjacent instability (7%), and nerve root injury (2%).

Back pain after thoracolumbar fracture treated with long instrumentation and short fusion.

The purposes of this retrospective study are to evaluate the degeneration of facets in unfused segments immobilized by Harrington rods and to see how often back pain developed after treatment with this method. Sixty patients with thoracolumbar fracture were treated with the Harrington rod long-fuse short technique. Harrington rods are removed an average of 14 months after initial surgery. Every case showed solid fusion during implant removal exploration. Thereafter, all cases were followed for a minimum of 5 years. Roentgenographic studies and a bone scan were performed to evaluate the degeneration of facet joints. Back pain complaint was found in 11 patients (18.3%). The degeneration of unfused facets was visualized in six patients by roentgenograms. Increased activity in the instrumented spine by bone scan examination also was noted in six patients: three cases were at unfused area, one at a fused area, and two at fused and unfused areas. In conclusion, degeneration in the immobilized and unfused segments after rod long and fused short was rarely seen clinically after long-term follow-up. Back pain was experienced in only 18.3% of the patients and seldom required medication.