ABSTRACT
Rhodobryum laxelimbatum (Bryophyta, Bryaceae) is one of the folk medicine resources in Southwest China, which has excellent potential for application in treating cardiovascular diseases. In this study, R. laxelimbatum was sequenced by high-throughput sequencing technology. The complete chloroplast genome is 124,632 bp in length with a quadripartite structure. Two inverted repeat regions are 9837 bp, separated by a large single copy region of 86,444 bp and a small single copy region of 18,514 bp. It encodes 118 unique genes, including 82 protein-coding genes, 32 tRNA genes, and four rRNA genes. The phylogenetic tree was constructed based on the complete chloroplast genome sequences of 18 bryophytes, downloaded from GenBank and acquired in this study. The phylogenetic analysis strongly indicated that R. laxelimbatum was the sister group of the clade which consists of Mnium marginatum, Pohlia cruda and Pohlia nutans. The R. laxelimbatum chloroplast genome sequence provides new genomic resources, which will improve its research, conservation, and application in the future.
ABSTRACT
Mnium is the type genus of the family Mniaceae, the species' richest family of order Bryales (Bryophyte). Mnium marginatum is widely distributed worldwide. The complete chloroplast genome of M. marginatum was sequenced by high-throughput sequencing technology. The chloroplast genome is 124,935 bp in length with a quadripartite structure. The two inverted repeat regions are 9,910 bp long and separated by a large single-copy region of 86,572 bp and a small single-copy region of 18,543 bp. Based on the chloroplast genome data of 11 bryophytes downloaded from GenBank and one acquired in this study, phylogenetic trees were constructed.
ABSTRACT
Pohlia is a genus with many taxonomic and systematic controversies. In this study, the complete chloroplast genome of Pohlia cruda (Bryales, Bryophyte) was sequenced by high-throughput sequencing technology and described. The complete chloroplast genome is 125,114 bp in length and has a quadripartite structure. The two inverted repeat (IR) regions are 9921 bp long and separated by a large single-copy (LSC) region of 86,727 bp and a small single-copy (SSC) region of 18,545 bp. Phylogenetic trees were constructed based on the complete chloroplast genome sequences of 10 bryophytes downloaded from GenBank and one acquired in this study.
ABSTRACT
To investigate the seroprevalence of Toxoplasma gondii infection in domestic cats in central China, 843 serum samples were collected in Henan province between March 2015 and May 2016 and tested for IgG antibodies against T. gondii using the enzyme-linked immunosorbent assay (ELISA). The overall seroprevalence of T. gondii was 21% (178/843). No significant difference was observed based on the sex of cats (p > 0.05). Significantly higher seroprevalence (p < 0.05) was observed in mixed-breed cats (24%) compared to purebred cats (17%). Seroprevalence in rural cats (29%) was significantly higher (p < 0.01) than in urban cats (16%), and increased significantly (p < 0.01) with age. These results showed that T. gondii was highly prevalent in domestic cats in Henan province, central China, which might have important implications for public health.