Measuring the dynamics of leaf area index of vegetation using fisheye camera.

The rapid and accurate measurement of LAI is of great importance for the research of ecological processes. Photos from typical land use types in the northern Loess Plateau, including Caragana, Salix, alfalfa, wild grass, soybean and maize, were measured by digital hemispherical photography (DHP). Meanwhile, photos were daily taken by video camera with fisheye lens and the pictures were analyzed by image processing software to obtain the dynamics of LAI in soybean, maize and Caragana fields. The results showed that a linear correlation existed between the LAI measured by DHP and LAI-2200. The coefficient of determination (R2) was 0.85 (P<0.05) and root mean square error (RMSE) was 0.256. The key parameters of professional software were affected by the local solar radiation. When the downward lens was used, the green index was the key parameter which increased with the increase of solar radiation. However, the brightness index decreased with the increase of solar radiation when the lens was upward. Through the adjustment of the key parameters, the results of LAI of maize, soybean, and Caragana were consistent with the LAI-2200 results, well reflecting LAI dynamics during the plant growth. The downward lens in Caragana field was better. The fisheye camera could be used for monitoring the dynamic LAI of different vegetations.

Water-soluble constituents of the heartwood of Streblus asper.

A new lignan, (7'R,8'R)-threo-strebluslignanol-2-O-beta-D-glucopyranoside, along with 8 known compounds (2-9) were isolated from the water-soluble part of the MeOH extract of the heartwood of Streblus asper. Their structures were elucidated through various spectroscopic methods, including 1D NMR (1H NMR, 13C NMR), 2D NMR (HMQC, HMBC, and NOESY), and HRMS. The stereochemistry at the chiral centers was determined using the CD spectrum, as well as analyses of coupling constants and optical rotation data. In the preliminary bioassay, the isolated compounds did not show anti-HBV activities in vitro using the HBV transfected HepG2.2.15 cell line.

Anti-HBV activities of Streblus asper and constituents of its roots.

The extracts from leaves, heartwood, barks and roots of the Streblus asper were investigated for anti-HBV activities, separately. The results suggested that the MeOH extracts of the heartwood, barks, and roots exhibited good anti-HBV activities. Further investigations displayed that ethyl acetate and n-butanol soluble parts of their MeOH extracts showed more significant anti-HBV activities. Moreover, a new lignan, together with 11 known compounds, was isolated from n-butanol-soluble part of MeOH extract of the roots of S. asper. The structures were elucidated by spectroscopic methods, including 1D NMR ((1)H NMR, (13)C NMR), 2D NMR (HMQC, HMBC) and HR-EI-MS experiments. Compounds 1-3 were evaluated for their anti-HBV activities. Honokiol showed significant anti-HBV activity with IC(50) values of 3.14µM and 4.74µM for HBsAg and HBeAg with no cytotoxicity respectively, while lamivudine (3TC, positive control) exhibited weak anti-HBV activity with IC(50) values of 11.81µM and 25.80µM for HBsAg and HBeAg respectively.