ABSTRACT
We investigated whether pre- and postnatal low-dose exposure to diesel exhaust (DE) affects male reproductive function in mice. The DE concentration is less than that indicated as the environmental quality standard for suspended particulate matter (SPM) in Japan. ICR mice were exposed prenatally to low-dose diesel exhaust (0.17 mg of DE particles/m³) through the airway for 8 h/day in an exposure chamber from gestational day 2 until the examination. In the DE-exposed groups, normal sperm morphology in the epididymis was reduced (p < 0.01), and seminiferous tubules showed degenerative changes in which the number of Sertoli cells was decreased (p < 0.01). Those changes were observed at 6 and 12 weeks of age. Furthermore, ultrastructural studies revealed an increase in damaged mitochondria in Sertoli cells (p < 0.001) and variform spermatozoa. These results indicate that pre- and postnatal exposure of low-dose DE is detrimental to Sertoli cell function and may cause abnormal spermatozoa.
Subject(s)
Air Pollutants/toxicity , Maternal Exposure/adverse effects , Prenatal Exposure Delayed Effects , Spermatogenesis/drug effects , Spermatozoa/drug effects , Vehicle Emissions/toxicity , Animals , Female , Male , Mice , Mice, Inbred ICR , Microscopy, Electron , Mitochondria/drug effects , Mitochondria/ultrastructure , Pregnancy , Seminiferous Tubules/drug effects , Seminiferous Tubules/pathology , Sertoli Cells/drug effects , Sertoli Cells/pathology , Sertoli Cells/ultrastructure , Spermatozoa/pathology , Spermatozoa/ultrastructureABSTRACT
We recently showed that prenatal exposure to diesel exhaust (DE) disrupts spermatogenesis in mouse offspring. This study was undertaken to determine whether filtered DE in which 99.97% of diesel exhaust particles >0.3 microm in diameter were removed affects spermatogenesis in growing mice. After prenatal exposure to filtered DE for 2-16 days postcoitum, we examined daily sperm production (DSP), testicular histology, serum testosterone levels and mRNA expression of hormone synthesis process-related factors. In the filtered DE exposed group, DSP was markedly reduced at 12 weeks compared with the control group; clean air exposed group. Histological examination showed multinucleated giant cells and partial vacuolation in the seminiferous tubules of the exposed group. Testosterone was elevated significantly at 5 weeks. Moreover, luteinizing hormone receptor mRNA at 5 and 12 weeks, 17alpha-hydroxylase/C17-20-lyase and 17beta-hydroxysteroid dehydrogenase mRNAs at 12 weeks were significantly elevated. These results suggest that filtered DE retains its toxic effects on the male reproductive system following prenatal exposure.
Subject(s)
Prenatal Exposure Delayed Effects , Spermatogenesis/drug effects , Testis/drug effects , Vehicle Emissions/toxicity , 17-Hydroxysteroid Dehydrogenases/drug effects , 17-Hydroxysteroid Dehydrogenases/metabolism , Animals , Female , Gene Expression Regulation/drug effects , Giant Cells/drug effects , Giant Cells/metabolism , Male , Mice , Mice, Inbred ICR , Particle Size , Pregnancy , RNA, Messenger/drug effects , RNA, Messenger/metabolism , Receptors, LH/drug effects , Receptors, LH/metabolism , Seminiferous Tubules/drug effects , Sperm Count , Steroid 17-alpha-Hydroxylase/drug effects , Steroid 17-alpha-Hydroxylase/metabolism , Testis/metabolism , Testosterone/blood , Time Factors , Vacuoles/drug effects , Vacuoles/metabolismABSTRACT
The effect of prenatal exposure to diesel exhaust (DE) was investigated. Twenty pregnant ICR mice were exposed to DE at the particle concentration of 1.0 mg/m3, from d 2 until d 16 postcoitum. Male offspring were kept alive until 12 wk of age, and then male reproductive organ weight, daily sperm production (DSP), serum testosterone level, and mRNA expression of sex steroid hormone synthesis process-related factors were measured. Serum testosterone levels of the exposed group were reduced significantly at 3 wk, whereas they were elevated significantly at 12 wk. DSP was also markedly reduced at 5 and 12 wk. Histological examination showed multinucleated giant cells in the seminiferous tubules of the exposed group as well as partial vacuolation of the seminiferous tubules. Follicle-stimulating hormone receptor (FSHR) mRNA expression and steroidogenesis acute regulatory (StAR) protein were significantly increased at 5 wk and 12 wk, respectively. This study suggests that prenatal exposure to DE has detrimental effects on mouse spermatogenesis in offspring.
