ABSTRACT
Earthworms are the most abundant invertebrates in the soils and are permanently in close contact with soil particles. Therefore, they are significantly affected by the pollutants that reach the soil system. The study was aimed at evaluating the effect of exposure to crude oil contaminated soil on Eisenia fetida using cellular antioxidant enzymes and tissue organization as biomarkers. E. fetida were exposed to different concentrations of crude oil contamination of 1 mL, 2 mL. and 3 mL (0.25, 0.50 and 0.75%) for 14 days. The antioxidant/oxidant parameters were analysed in the muscle and liver tissues. The results showed that only the reduced glutathione (GSH) of earthworms exposed to 1 mL were not significant (p>0.05) from the control, while the other concentrations (2 mL - 0.50% and 3 mL - 0.75%) were significantly different (p<0.05) from the control. The activity of catalase (CAT) with respect to the total protein content was highest in the liver of earthworms exposed to 1 mL (0.25% conc.) on day 7 (51.84 µ mol/mg pro) while the least CAT activity with respect to the total protein content was reported in the liver of control earthworms after 14 days (19.51 µ mol/mL/min). A significant increase in the activity of superoxide dismutase at all the concentrations (0.25, 0.50 and 0.75%) after 14 days were also observed. Significant histopathological alterations were observed in E. fetida from the three concentrations. Severe disruptions in the arrangement of their body wall muscle layers, distorted internal viscera, as well as cellular degeneration, pigments, moderate to severe areas of lesion, and distortion of the shape of circular and longitudinal muscles, eroding of internal and external tissues leading to total destruction of body wall were observed. In conclusion, the study revealed that crude oil even at lower concentration induced biomarker responses in E. fetida such as higher levels of Malondialdehyde in E. fetida after exposure crude oil due its toxicity. Histopathological alterations such as cellular degeneration, moderate to severe areas of necrosis, areas of inflammation, inclusion bodies, pigments, and distortion of the shapes of circular and longitudinal muscles also showed the adverse impacts of crude oil pollution in the soils.
ABSTRACT
INTRODUCTION: In spite of doubts over the safety and nutritional benefits of singed cowhide meat, called ponmo in some parts of Nigeria, and its potential negative impact on the hide and leather industry, consumption in Nigeria and many parts of Africa has continued unabated. OBJECTIVES: In the present study, physicochemical and genotoxic assessments of wastewater used to rinse ready-to-cook singed cowhide meat were carried out. METHODS: Physicochemical analyses were carried out using the American Public Health Association procedures, while genotoxic assessment was carried out using Allium cepa chromosome assay. RESULTS: The results of the physicochemical analyses indicated that some of the parameters, especially metals, were within the threshold of the limits set by the country's regulatory agencies, but some parameters like phosphate, chloride, nitrate, biological oxygen demand, and chemical oxygen demand were higher in concentration. The wastewater inhibited the growth of A. cepa roots and caused a decrease in its mitotic index relative to the control onions exposed to water only. The highest root growth inhibition of 72.14% was induced by a 10% wastewater concentration, while the lowest (53.57%) was induced by a 5% wastewater concentration. In addition, the wastewater induced bi-nucleated, attached, vagrant, C-mitosis, sticky and bridged chromosomal aberrations. Wastewater at a 5% concentration induced the highest significant (P < 0.05) percentage chromosome aberration of 36.62% at 48 hours of exposure. Sticky chromosomes had the highest significant frequency (P <0.01) at the end of the 72-hour exposure period. No chromosomal aberration was observed in the control. CONCLUSIONS: These results indicate that singed cowhide meat wastewater is potentially genotoxic and environmentally harmful. Governments, public health practitioners, and relevant stakeholders should work in synergy to discourage the habit of processing cowhide into cowhide meat. COMPETING INTERESTS: The authors declare no competing financial interests.
