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1.
J Immunol ; 185(2): 1124-31, 2010 Jul 15.
Article in English | MEDLINE | ID: mdl-20543103

ABSTRACT

Various bacterial pathogens activate the endothelium to secrete proinflammatory cytokines and recruit circulating leukocytes. In contrast, there is a distinct lack of activation of these cells by Francisella tularensis, the causative agent of tularemia. Given the importance of endothelial cells in facilitating innate immunity, we investigated the ability of the attenuated live vaccine strain and virulent Schu S4 strain of F. tularensis to inhibit the proinflammatory response of HUVECs. Living F. tularensis live vaccine strain and Schu S4 did not stimulate secretion of the chemokine CCL2 by HUVECs, whereas material released from heat-killed bacteria did. Furthermore, the living bacteria suppressed secretion in response to heat-killed F. tularensis. This phenomenon was dose and contact dependent, and it occurred rapidly upon infection. The living bacteria did not inhibit the activation of HUVECs by Escherichia coli LPS, highlighting the specificity of this suppression. The endothelial protein C receptor (EPCR) confers anti-inflammatory properties when bound by activated protein C. When the EPCR was blocked, F. tularensis lost the ability to suppress activation of HUVECs. To our knowledge, this is the first report that a bacterial pathogen inhibits the host immune response via the EPCR. Endothelial cells are a critical component of the innate immune response to infection, and suppression of their activation by F. tularensis is likely a mechanism that aids in bacterial dissemination and evasion of host defenses.


Subject(s)
Antigens, CD/immunology , Endothelial Cells/immunology , Francisella tularensis/immunology , Receptors, Cell Surface/immunology , Antibodies, Monoclonal/immunology , Antibodies, Monoclonal/pharmacology , Antigens, CD/metabolism , Cells, Cultured , Chemokine CCL2/immunology , Chemokine CCL2/metabolism , Endothelial Cells/metabolism , Endothelial Cells/microbiology , Endothelial Protein C Receptor , Enzyme-Linked Immunosorbent Assay , Francisella tularensis/physiology , Host-Pathogen Interactions/drug effects , Host-Pathogen Interactions/immunology , Humans , Inflammation Mediators/immunology , Inflammation Mediators/metabolism , Interleukin-8/immunology , Interleukin-8/metabolism , Receptors, Cell Surface/metabolism , Time Factors , Vaccines, Attenuated/immunology
2.
Infect Immun ; 78(4): 1797-806, 2010 Apr.
Article in English | MEDLINE | ID: mdl-20123721

ABSTRACT

Francisella tularensis, the causative agent of tularemia, interacts with host cells of innate immunity in an atypical manner. For most Gram-negative bacteria, the release of lipopolysaccharide (LPS) from their outer membranes stimulates an inflammatory response. When LPS from the attenuated live vaccine strain (LVS) or the highly virulent Schu S4 strain of F. tularensis was incubated with human umbilical vein endothelial cells, neither species of LPS induced expression of the adhesion molecule E-selectin or secretion of the chemokine CCL2. Moreover, a high concentration (10 microg/ml) of LVS or Schu S4 LPS was required to stimulate production of CCL2 by human monocyte-derived macrophages (huMDM). A screen for alternative proinflammatory factors of F. tularensis LVS identified the heat shock protein GroEL as a potential candidate. Recombinant LVS GroEL at a concentration of 10 microg/ml elicited secretion of CXCL8 and CCL2 by huMDM through a TLR4-dependent mechanism. When 1 microg of LVS GroEL/ml was added to an equivalent amount of LVS LPS, the two components synergistically activated the huMDM to produce CXCL8. Schu S4 GroEL was less stimulatory than LVS GroEL and showed a lesser degree of synergy when combined with Schu S4 LPS. These findings suggest that the intrinsically low proinflammatory activity of F. tularensis LPS may be increased in the infected human host through interactions with other components of the bacterium.


Subject(s)
Chaperonin 60/immunology , Francisella tularensis/immunology , Lipopolysaccharides/immunology , Macrophage Activation , Macrophages/immunology , Animals , Bacterial Vaccines/immunology , Chemokine CCL2/metabolism , Female , Humans , Interleukin-8/metabolism , Mice , Mice, Inbred C57BL
3.
Microb Pathog ; 44(6): 512-23, 2008 Jun.
Article in English | MEDLINE | ID: mdl-18304778

ABSTRACT

Francisella tularensis is a highly virulent bacterium that causes tularemia, a disease that is often fatal if untreated. A live vaccine strain (LVS) of this bacterium is attenuated for virulence in humans but produces lethal disease in mice. F. tularensis has been classified as a Category A agent of bioterrorism. Despite this categorization, little is known about the components of the organism that are responsible for causing disease in its hosts. Here, we report the deletion of a well-characterized lipoprotein of F. tularensis, designated LpnA (also known as Tul4), in the LVS. An LpnA deletion mutant was comparable to the wild-type strain in its ability to grow intracellularly and cause lethal disease in mice. Additionally, mice inoculated with a sublethal dose of the mutant strain were afforded the same protection against a subsequent lethal challenge with the LVS as were mice initially administered a sublethal dose of the wild-type bacterium. The LpnA-deficient strain showed an equivalent ability to promote secretion of chemokines by human monocyte-derived macrophages as its wild-type counterpart. However, recombinant LpnA potently stimulated primary cultures of human macrophages in a Toll-like receptor 2-dependent manner. Although human endothelial cells were also activated by recombinant LpnA, their response was relatively modest. LpnA is clearly unnecessary for multiple functions of the LVS, but its inflammatory capacity implicates it and other Francisella lipoproteins as potentially important to the pathogenesis of tularemia.


Subject(s)
Bacterial Proteins/immunology , Bacterial Vaccines/immunology , Francisella tularensis/immunology , Francisella tularensis/pathogenicity , Inflammation Mediators/immunology , Lipoproteins/immunology , Tularemia/immunology , Animals , Bacterial Proteins/genetics , Bacterial Vaccines/genetics , Chemokines/immunology , Conjugation, Genetic , Endothelial Cells/immunology , Endothelial Cells/microbiology , Female , Francisella tularensis/genetics , Francisella tularensis/metabolism , Humans , Lipoproteins/genetics , Macrophage Activation , Macrophages/immunology , Macrophages/microbiology , Male , Mice , Mice, Inbred C3H , Sequence Deletion , Tularemia/microbiology , Vaccines, Attenuated/genetics , Vaccines, Attenuated/immunology , Virulence
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