ABSTRACT
The aim of this study was to investigate the effect of anethole (AN) and eugenol (EUG) on leukocyte migration using in vitro chemotaxis and in situ microcirculation assays. BALB/c mice were used for the in vitro chemotaxis assay, and Wistar rats for the in situ microcirculation assay. We evaluated (a) the in vitro leukocyte migration in response to chemotactic factors (formyl-methionyl-leucyl-phenylalanine [fMLP] and leukotriene B4 [LTB4]) and (b) the rolling, adhesion, and migration of leukocytes induced by an injection of carrageenan (100 µg/cavity) into the scrotum of the animal. In the in vitro chemotaxis assay, AN and EUG at doses of 1, 3, 9, and 27 µg/ml significantly inhibited leukocyte migration when stimulated by the chemotactic agents fMLP and LTB4. In the in situ microcirculation assay, AN at doses of 125 and 250 mg/kg and EUG at a dose of 250 mg/kg significantly decreased the number of leukocytes that rolled, adhered, and migrated to perivascular tissue. The results indicate that AN and EUG exert inhibitory effects on leukocyte migration, highlighting their possible use to diminish excessive leukocyte migration in the inflammatory process.
Subject(s)
Anisoles/pharmacology , Chemotaxis, Leukocyte/drug effects , Eugenol/pharmacology , Allylbenzene Derivatives , Animals , Carrageenan/pharmacology , Humans , Leukocytes/drug effects , Leukocytes/physiology , Leukotriene B4/pharmacology , Male , Mice , Mice, Inbred BALB C , N-Formylmethionine Leucyl-Phenylalanine/pharmacology , Rats , Rats, WistarABSTRACT
Rosmarinus officinalis L. (Lamiaceae), popularly known as rosemary, is used for food flavoring and in folk medicine as an antispasmodic, analgesic, antirheumatic, diuretic, and antiepileptic agent. Few studies have shown the anti-inflammatory effects of rosemary essential oil (REO). This study evaluated the effects of REO on leukocyte migration through in vivo leukocyte migration and in vitro chemotaxis assay. REO was analyzed by using gas chromatography-mass spectometry, and the main components identified were camphor (27.59%), 1,8-cineole (15.74%), α-pinene (16.58%), and ß-myrcene (10.02%). In rats, administration of REO reduced the number of leukocytes that rolled, adhered, and migrated to the scrotal chamber after carrageenan injection. All doses of REO tested significantly inhibited leukocyte chemotaxis induced by casein. The effects of REO on leukocyte migration highlight an important mechanism of the anti-inflammatory action of rosemary.
Subject(s)
Anti-Inflammatory Agents, Non-Steroidal/pharmacology , Cell Migration Inhibition , Chemotaxis, Leukocyte/drug effects , Leukocytes/drug effects , Oils, Volatile/pharmacology , Animals , Anti-Inflammatory Agents, Non-Steroidal/administration & dosage , Anti-Inflammatory Agents, Non-Steroidal/chemistry , Carrageenan/toxicity , Cell Migration Assays, Leukocyte , Dose-Response Relationship, Drug , Endothelium/cytology , Endothelium/drug effects , Endothelium/immunology , Gas Chromatography-Mass Spectrometry , Leukocyte Adherence Inhibition Test , Leukocytes/cytology , Leukocytes/immunology , Magnetic Resonance Spectroscopy , Male , Medicine, Traditional , Monoterpenes/analysis , Monoterpenes/chemistry , Monoterpenes/pharmacology , Oils, Volatile/administration & dosage , Oils, Volatile/chemistry , Plant Leaves/chemistry , Rats , Rats, Wistar , Rosmarinus/chemistry , Scrotum/cytology , Scrotum/drug effects , Scrotum/immunologyABSTRACT
Zingiber officinale Roscoe, popular name ginger, is grown naturally in many parts of the world, including Brazil. Ginger is used in pharmaceutical, cosmetic, and food and beverage industries and the essential oil has been used in folk medicine for manifold conditions including as an analgesic, anti-inflammatory, and antirheumatic. The purpose of this study was to investigate the effects of ginger (Zingiber officinale Roscoe) essential oil (GEO) in an in vitro chemotaxis assay and on leukocyte-endothelial interactions in vivo. GEO was analyzed by GC-MS and the main components identified were ar-curcumene (59%), ß-myrcene (14%), 1,8-cineol (8%), citral (7.5%), and zingiberene (7.5%). Oral administration of GEO (200-500 mg/kg) reduced the rolling and leukocyte adherence after 2 h of carrageenan injection (100 µg) into the scrotal chamber. The number of leukocytes migrated to the perivascular tissue 4 h after the irritant stimulus was also diminished. GEO in all doses tested (10(-4), 10(-3), or 10(-2) µL/mL) caused a significant reduction of leukocyte chemotaxis (35.89 ± 4.33, 30.67 ± 0.70, and 35.85 ± 3.83%, respectively) toward casein stimuli. The data presented showed direct and systemic effects of GEO on leukocyte migration as an important mechanism of the anti-inflammatory action of ginger.
