ABSTRACT
Mycelium of Streptomyces cinnamonensis mutant strains cultivated in a synthetic medium with glycine produced protoplasts after lysis of cell walls with lysozyme. The protoplast yield was up to 95%. The protoplasts could revert and mycelial forms were thus regenerated. In a sucrose-containing medium the protoplasts stored at 4 degrees C were stable for 2 d.
Subject(s)
Streptomyces/genetics , Culture Media , Glycine , Muramidase , Mutation , Mycology/methods , Protoplasts/cytology , Protoplasts/physiology , Streptomyces/cytology , Streptomyces/physiologyABSTRACT
Alizarin glucosyl transferase activity was found in five mutant strains of Streptomyces aureofaciens. The activity bears no direct relationship to the final products of tetracycline biosynthesis.
Subject(s)
Anthraquinones/pharmacology , Glucosyltransferases/metabolism , Streptomyces aureofaciens/enzymology , Glucosides/metabolism , Glucosyltransferases/isolation & purification , Mutagenesis , Streptomyces aureofaciens/geneticsABSTRACT
Vitamins added to submerged Streptomyces cinnamonensis cultures stimulated the production of monensins. Vitamins B2, B3, B5 and B12 enhanced the production by about 50%, vitamins B1 and B6 by 100%. The addition of biotin in optimal concentration resulted in more than 3-fold increase in total production.
Subject(s)
Biotin/pharmacology , Monensin/biosynthesis , Streptomyces/metabolism , Vitamin B Complex/pharmacology , Culture Media , Niacin/pharmacology , Pantothenic Acid/pharmacology , Pyridoxine/pharmacology , Riboflavin/pharmacology , Streptomyces/drug effects , Thiamine/pharmacology , Vitamin B 12/pharmacologyABSTRACT
Variants resistant to propionate were prepared from a mutant strain of Streptomyces cinnamonensis producing predominantly monensin A. Using selected resistants the production of monensins (in media with higher concentrations of propionate) was examined. Stimulation of monensin synthesis by propionate was observed with 70% of the resistants studied. Propionate did not influence the ratio between monensin A and B production.
Subject(s)
Monensin/biosynthesis , Propionates/pharmacology , Streptomyces/metabolism , Drug Resistance, Microbial , MutationABSTRACT
A diffusion plate method for the assay of the activity of lipases in large series of samples was worked out. When using this method the maximum deviation was found to be +/- 3.8%.
Subject(s)
Candida/enzymology , Lipase/analysis , Streptomyces/enzymology , Animals , Diffusion , Kinetics , Methods , Pancreas/enzymology , SwineABSTRACT
In addition to typical acetyl esters, acetic ester acetyl-hydrolase from Mycobacterium phlei can degrade aliphatic acetylated amino acids under suitable conditions.
Subject(s)
Amino Acids/metabolism , Esterases/pharmacology , Mycobacterium phlei/enzymology , Mycobacterium/enzymology , Acetylation , HydrolysisABSTRACT
When investigating the effect of aeration on the utilization of a lipid substrate from the cultivation broth and production of salinomycin in Streptomyces albus it was demonstrated that a higher aeration results in a better utilization of soya oil and a higher production of the antibiotic.
