KIF24 depletion induces clustering of supernumerary centrosomes in PDAC cells.

Clustering of supernumerary centrosomes, which potentially leads to cell survival and chromosomal instability, is frequently observed in cancers. However, the molecular mechanisms that control centrosome clustering remain largely unknown. The centrosomal kinesin KIF24 was previously shown to restrain the assembly of primary cilia in mammalian cells. Here, we revealed that KIF24 depletion suppresses multipolar spindle formation by clustering centrosomes in pancreatic ductal adenocarcinoma (PDAC) cells harboring supernumerary centrosomes. KIF24 depletion also induced hyper-proliferation and improved mitotic progression in PDAC cells. In contrast, disruption of primary cilia failed to affect the proliferation and spindle formation in KIF24-depleted cells. These results suggest a novel role for KIF24 in suppressing centrosome clustering independent of primary ciliation in centrosome-amplified PDAC cells.

Changes of residual stresses on the surface of leucite-reinforced ceramic restoration luted with resin composite cements during aging in water.

The objective of this study was to compare the changes in the residual stresses present on the surface of leucite-reinforced dental ceramic restorations luted with a self-adhesive and a conventional resin composite cement during aging in water. Ring specimens made of a leucite-reinforced ceramics were luted to ceramic cylinders using a self-adhesive (Panavia SA Luting Plus) or a conventional resin composite cement (Panavia V5) in dual-cure or self-cure mode. Residual stresses on the ring surface were measured using indentation fracture method at 1 h, 1, 3, 7, 14 and 28 days of the 37 °C water immersion. Water sorption, water solubility and elastic modulus of the cements were also measured. Compressive stress was generated on the surface of the ceramic rings by the polymerization of the resin composite cements, and the stresses appeared to decrease over time by water sorption of the cements. The dual-cured conventional resin composite cement remained compressive stresses on the ceramic surface, while only the self-cured self-adhesive cement, which demonstrated the greatest water sorption, generated tensile stresses during the four weeks of aging in water. The elastic moduli of cements did not significantly change through the immersion, suggesting that the stresses were less affected by the modulus. To prevent the generation of tensile stresses on the leucite-reinforced ceramic restoration, self-adhesive cements exhibiting small water sorption should be clinically selected.