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Background and Aim: Coat color variations in cattle are known to be influenced by the melanocortin 1 receptor (MC1R) and receptor tyrosine kinase (KIT) genes. The presence of coat color abnormalities, such as white spots and albinism, in Bali cattle was the focus of this study. This study aimed to identify single nucleotide polymorphisms (SNPs) in the coding region of MC1R and exons 2 and 3 of KIT associated with coat color abnormalities in Bali cattle. Materials and Methods: The study included the analysis of 48 Bali cattle, including 20 individuals with standard coat color, 15 with white spots, and 13 with albinism. Total DNA was extracted using a DNA Extraction Kit, and MC1R (coding region) and KIT (exons 2 and 3) gene amplifications were analyzed using forward and reverse primers with polymerase chain reaction product lengths of 1071, 234, and 448 bp, respectively. The determination of MC1R and KIT gene diversity was analyzed through direct sequencing. Melanocortin 1 receptor and KIT gene sequence data were analyzed using BioEdit and MEGA6 to identify SNPs associated with standard and abnormal coat color phenotypes (white-spotted and albino) in Bali cattle. Results: No SNPs associated with coat color abnormalities were found in the coding region of MC1R and exons 2 and 3 of KIT genes in Bali cattle. However, the intron two regions of KIT contained the SNP g.70208534A>G, which showed a high degree of diversity. The AA genotype frequency was highest in albino Bali cattle, whereas the G allele frequency was highest and the A allele frequency was lowest in white-spotted Bali cattle. Conclusion: The results indicated that standard, white-spotted, and albino coat colors in Bali cattle could not be distinguished by analyzing the MC1R and KIT genes.
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BACKGROUND: Sheep is one of the commodities of livestock which has been known widely in Indonesia for supporting the national food security. Improvement in genetic quality by selection based on genetic markers for growth is necessary to increase meat production. Quantitative trait loci (QTL) analysis in sheep suggests that Calpain 3 gene (CAPN3) gene might be one of the candidate loci affecting growth traits. CAPN3 is located on chromosome 7 sheep expressed in the skeletal muscles. The aim of this study was to investigate polymorphism CAPN3 intron 11 in Merino × Garut (MEGA) backcross using the PCR-RLFP method and to determine their association with growth traits. RESULTS: SNP intron 11 CAPN3 | BseSI of Merino × Garut (MEGA) backcross sheep was polymorphic and resulted in two alleles of C and T with a frequency of 0.76 and 0.24, respectively, and CC, CT, and TT genotypes with a frequency of 0.54, 0.43, and 0.02, respectively. These loci were found to be in Hardy-Weinberg equilibrium. The SNP CAPN3 | BseSI significantly affected (P < 0.05) the birth weight in Merino × Garut (MEGA) backcross sheep. CONCLUSION: This result suggests that the CAPN3 | BseSI can be used as a genetic marker for birth weight trait in sheep.
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To assess their potential for beef production, this study describes the morphometrics, physical traits, and body weight of 1034 Indonesian beef cattle from eight breeds, namely Bali, Rambon, Madura, Ongole Grade, Kebumen Ongole Grade, Sasra, Jabres, and Pasundan. An analysis of variance in addition to cluster, Euclidean distance, dendrogram, discriminant function, stepwise linear regression, and morphological index analyses were performed to describe the differences in traits among breeds. The morphometric proximity analysis revealed two distinct clusters with a common ancestor, where the first cluster included Jabres, Pasundan, Rambon, Bali, and Madura cattle and the second included Ongole Grade, Kebumen Ongole Grade, and Sasra cattle, with an average suitability value of 93.20â¯%. This showed that the classification and validation methods can be used to distinguish breeds. The most important factor in estimating body weight was the heart girth circumference. Ongole Grade cattle had the highest cumulative index, followed by Sasra, Kebumen Ongole Grade, Rambon, and Bali cattle. A cumulative index value >3 can be used as a threshold for determining the type and function of beef cattle.
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OBJECTIVE: This study aimed to investigate the polymorphisms of the dopa decarboxylase (DDC) gene and association analysis with lamb quality and expression quantification of the DDC gene in phenotypically divergent Indonesian sheep. METHODS: The totals of 189 rams with an average body weight of 24.12 kg at 10 to 12 months were used to identify DDC gene polymorphism using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Among 189 rams, several rams representing various sheep genotypes were used for an association study between genotypes and phenotypic traits with proc general linear model (GLM) analysis. In addition, the gene expression analysis of the DDC mRNA in the phenotypically divergent sheep population was analyzed using quantitative reverse-transcription PCR. RESULTS: The DDC gene (g. 5377439 G>A) showed polymorphisms that indicated three genotypes: AA, AG, and GG. The DDC gene polymorphism was significantly associated (p≤0.05) with carcass characteristics including carcass percentage, carcass length, hot and cold carcass; physical properties of lamb quality including pH value; retail cut carcass; fatty acid composition such as fat content, pentadecanoic acid (C15:0), tricosylic acid (C23:0), lignoceric acid (C24:0), oleic acid (C18:1n9c), elaidic acid (C18:1n9t), nervonic acid (C24:1), linoleic acid (C18:2n6c), arachidonic acid (C20:4n6), cervonic acid (C22:6n3); and mineral content including potassium (K). The GG genotype of the DDC gene had the best association with lamb quality traits. The DDC gene expression analysis mRNA showed no significant difference (p≥0.05) between lamb quality traits. CONCLUSION: The DDC gene could be used as a potential candidate gene to improve lamb quality.
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Growth curves are important for understanding the growth phase and specific characteristics of farm animals. This study evaluated growth curve models to predict the age at puberty and growth rate of Ongole Grade cattle. The study used the weights of cattle from birth to 5 years old in 2011 to 2019 obtained from the Beef Cattle Research Station. The data were analyzed using four non-linear growth curve models: Brody, von Bertalanffy, logistic, and Gompertz. The coefficients of determination (R2) of the growth equation for the respective models were in the high range. The lowest Akaike's information criterion (AIC) value for males and females was obtained by the Brody and logistic models, respectively. Males with the von Bertalanffy, logistic, and Gompertz models had an estimated age and body weight at puberty of 12.56 months and 198.93 kg, 21.46 months and 298.43 kg, and 15.71 months and 235.49 kg, respectively, while females had an estimated age and body weight of 11.79 months and 166.44 kg, 13.94 months and 164.08 kg, and 11.76 months and 169.57 kg, respectively. The Brody function best fit the growth rate and exhibited the smallest mean deviation.
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Nonlinear Dynamics , Sexual Maturation , Animals , Body Weight , Cattle , Female , Logistic Models , Male , Models, BiologicalABSTRACT
The objective of this research was to find the basic data on genetic diversity of mtDNA D-Loop in Aceh cattle and its association with Bhutanese, Chinese, and Indian cattle. There were sixty samples of DNA which had been sequenced; i.e. Banda Aceh (11), Saree (20), and Indrapuri (29). To the best of our knowledge this is the first published data on the complete mitochondrial D-Loop sequence of Aceh cattle. Results show that Aceh cattle have the closest relationship to Bos indicus and have been influenced by Bos taurus. The closest genetic ranges among Aceh cattle, Bhutanese, Chinese, Indian and Zebu were Aceh-Zebu (0.0138), Aceh-Bhutanese (0.0156), Aceh-Chinese (0.0190) and Aceh-Indian (0.0193). D-Loop mtDNA analyses showed that there were 27 haplotypes in which twenty-one samples spread in haplotype 1, two samples were in haplotype 2, and the other four haplotypes had various samples in the range of three to seventeen samples. One sample of Aceh cattle from Saree has a closest maternal genetic with B. taurus. One of the four mutations among the star-shaped clusters on median joining network was a new specific haploid-group in Aceh cattle. From this finding it could be assumed that Aceh cattle form a specific haplotype and it can be conclude that Aceh cattle are animal genetic resources from Aceh in Sumatera Island that have to be preserved.
