Hydrogen production in microbial electrolysis cells with biocathodes.

Electroautotrophic microbes at biocathodes in microbial electrolysis cells (MECs) can catalyze the hydrogen evolution reaction with low energy demand, facilitating long-term stable performance through specific and renewable biocatalysts. However, MECs have not yet reached commercialization due to a lack of understanding of the optimal microbial strains and reactor configurations for achieving high performance. Here, we critically analyze the criteria for the inocula selection, with a focus on the effect of hydrogenase activity and microbe-electrode interactions. We also evaluate the impact of the reactor design and key parameters, such as membrane type, composition, and electrode surface area on internal resistance, mass transport, and pH imbalances within MECs. This analysis paves the way for advancements that could propel biocathode-assisted MECs toward scalable hydrogen gas production.

Integrated dark-fermentation-microbial electrosynthesis for efficient wastewater treatment and bioenergy recovery.

High ammonia concentration in wastewater can hinder methane production rate in anaerobic digestion (AD)-microbial electrosynthesis systems (ADMES). To address this issue, a dual-chamber reactor was fabricated using an anion exchange membrane (AEM) to separate the dark-fermentation (DF) and ADMES process, preventing ammonia migration from the DF chamber to the ADMES chamber. As a result, the DF-ADMES achieved a high methane yield based on chemical oxygen demand (COD) of 0.35 L CH4/gCOD compared to control operation AD (0.23 L CH4/gCOD) and ADMES (0.30 L CH4/gCOD). Additionally, hydrogen could be recovered from the DF chamber which improved the energy efficiency of the DF-ADMES reactor (91.7 %) as compared to control AD (53.4 %) and ADMES (71.9 %). Thus, a dual-chamber DF-ADMES with an AEM separator could be a feasible design for scalable treatment of high nitrogen-containing wastewater and high bioenergy recovery.

Copper foam supported g-C3N4-metal-organic framework bacteria biohybrid cathode catalyst for CO2 reduction in microbial electrosynthesis.

Microbial electrosynthesis (MES) presents a versatile approach for efficiently converting carbon dioxide (CO2) into valuable products. However, poor electron uptake by the microorganisms from the cathode severely limits the performance of MES. In this study, a graphitic carbon nitride (g-C3N4)-metal-organic framework (MOF) i.e. HKUST-1 composite was newly designed and synthesized as the cathode catalyst for MES operations. The physiochemical analysis such as X-ray diffraction, scanning electron microscopy (SEM), and X-ray fluorescence spectroscopy showed the successful synthesis of g-C3N4-HKUST-1, whereas electrochemical assessments revealed its enhanced kinetics for redox reactions. The g-C3N4-HKUST-1 composite displayed excellent biocompatibility to develop electroactive biohybrid catalyst for CO2 reduction. The MES with g-C3N4-HKUST-1 biohybrid demonstrated an excellent current uptake of 1.7 mA/cm2, which was noted higher as compared to the MES using g-C3N4 biohybrid (1.1 mA/cm2). Both the MESs could convert CO2 into acetic and isobutyric acid with a significantly higher yield of 0.46 g/L.d and 0.14 g/L.d respectively in MES with g-C3N4-HKUST-1 biohybrid and 0.27 g/L.d and 0.06 g/L.d, respectively in MES with g-C3N4 biohybrid. The findings of this study suggest that g-C3N4-HKUST-1 is a highly efficient catalytic material for biocathodes in MESs to significantly enhance the CO2 conversion.

Fundamentals and recent progress in bioelectrochemical system-assisted biohythane production.

Biohythane, a balanced mixture of 10%-30% v/v of hydrogen and 70%-90% v/v of methane, could be the backbone of an all-purpose future energy supply. Recently, bioelectrochemical systems (BES) became a new sensation among environmental biotechnology processes with the potential to sustainably generate biohythane. Therefore, to unleash its full potential for scaling up, researchers are consistently improving microbial metabolic pathways, novel reactors, and electrode designs. This review presents a detailed analysis of recently discovered fundamental mechanisms and science and engineering intervention of different strategies to improve the biohythane composition and production rate from BES. However, several milestones are to be achieved, for instance, improving electrode kinetics using efficient catalysts, engineered microbial communities, and improved reactor configurations, for commercializing this sustainable technology. Thus, a future perspective section is included to recommend novel research lines, mainly focusing on the microbial communities and the efficient electrocatalysts, to enhance reactor performance.

Accelerating anaerobic digestion process with novel single chamber microbial electrochemical systems with baffle.

A newly designed microbial electrochemical system (MES) with the addition of a baffle between the electrodes was integrated with the anaerobic digestion (AD) process for biogas upgradation. Novel MES configuration attained an increased methane production rate of 292.6 mL/L∙d and methane yield of 0.36 ± 0.006 [Formula: see text] /gCOD, which were higher than the values (185.3 mL/L∙d and 0.33 ± 0.009 [Formula: see text] /gCOD) from the MES operation without baffle, respectively. Moreover, the MES with baffle operation resulted in increased substrate removal (88.4 ± 0.5%) and less volatile fatty acids accumulation with a high energy efficiency of 99.6 %. Microbial community analysis revealed that acids metabolizing bacteria, Firmicutes, and Methanothrix were highly enriched in the cathode biofilm of MES with baffle. This study suggests that the baffle addition into the single chamber MES is beneficial to further improve the methanogenesis process for practical applications in the scaled-up MES-AD process.

A critical review on microbe-electrode interactions towards heavy metal ion detection using microbial fuel cell technology.

Implicit interaction of electroactive microbes with solid electrodes is an interesting phenomenon in nature, which supported development of bioelectrochemical systems (BESs), especially the microbial fuel cell (MFCs) for valorization of low-value waste streams into bioelectricity. Intriguingly, the metabolism of interacted microbes with electrode is affected by the microenvironment at electrodes, which influences the current response. For instance, when heavy metal ions (HMIs) are imposed in the medium, the current production decreases due to their intrinsic toxic effect. This event provides an immense opportunity to utilize MFC as a sensor to selectively detect HMIs in the environment, which has been explored vastly in recent decade. In this review, we have concisely discussed the microbial interaction with electrodes and mechanism of detection of HMIs using an MFC. Recent advancement in sensing elements and their application is elaborated with a future perspective section for follow-up research and development in this field.

Conductive magnetite nanoparticles trigger syntrophic methane production in single chamber microbial electrochemical systems.

Performance of methane-producing microbial electrochemical systems (MESs) is highly reliant on electron transfer efficiency from electrode to microorganisms and vice versa. In this study, magnetite nanoparticles were used as electron carriers to enhance extracellular electron transfer in single chamber MESs. The MES with magnetite exhibited the highest methane yield and current generation of 0.37 ±â€¯0.009 LCH4/gCOD and 9.6 mA, respectively among the tested reactors. The experimental data was observed to be highly consistent with modified Gompertz model results (R2 > 0.99), which also showed 74.2% and 22.1% enhanced methane production rate in MES with magnetite as compared to control AD and MES without magnetite, respectively. Cyclic voltammetry and electrochemical impedance spectroscopy analysis confirmed that magnetite enhanced catalytic activity of biofilm and lowered both solution and charge transfer resistance. Therefore, supplementing magnetite in MESs could be a strategy to develop an efficient syntrophic biomethanation in field scale applications.

Novel Sulfonated Co-poly(ether imide)s Containing Trifluoromethyl, Fluorenyl and Hydroxyl Groups for Enhanced Proton Exchange Membrane Properties: Application in Microbial Fuel Cell.

A hydroxyl group containing new cardo diamine monomer was synthesized, namely 9,9-bis (hydroxy- (4'-amino(3-trifluoromethyl)biphenyl-4-oxy)-phenyl)-9H-fluorene (mixture of isomers, HAPHPF). HAPHPF, along with a sulfonated diamine monomer, 4,4'-diaminostilbene-2,2'-disulfonic acid (DSDSA), was used to prepare a series of new sulfonated copolyimides by polycondensation with 1,4,5,8-naphthalenetetracarboxylic dianhydride (NTDA). The degree of sulfonation (DS < 1) was adjusted by the feed ratio of DSDSA/HAPHPF and the copolymers were named as DHN-XX, where XX denotes the mole percentage of DSDSA (XX = 50, 60, and 70). The copolymers showed high molecular weights. The copolymer structure and composition were confirmed by FTIR and NMR techniques. Copolymer membranes were prepared through solution cast route by using dimethyl sulfoxide as a solvent. The membranes showed high thermal, mechanical, hydrolytic and peroxide radical stability, and low water uptake and low swelling ratios. Well-separated hydrophilic and hydrophobic phase morphology was observed in TEM and AFM images of the copolymer membranes and was further supported by the SAXS studies. The proton conductivity of the DHN-70 was as high as 97 mS cm-1 at 80 °C and the value is significantly higher than that of the nonhydroxylated analogue. The membranes also showed superior microbial fuel cell (MFC) performance, similar like Nafion 117 under similar test conditions. The chemical oxygen demand removal values provide substantial evidence that the fabricated membranes can be utilized in bioelectrochemical systems.