Enclosure design for flock-level, chronic exposure of birds to air contaminant mixtures.

The objective of this study was to design an enclosure suitable for studying the ecotoxicological effects of vehicle emissions on groups of wild birds without compromising welfare. Two, adjacent enclosures sheltered from sunlight, wind and rain, were bird-proofed and wrapped with thick polyethylene sheeting. Emissions were directed into the treatment enclosure from the exhaust of a light-duty gasoline truck, using flexible, heat-proof pipe, with joins sealed to prevent leakage. During active exposure, the engine was idled for 5 h/day, 6 days/week for 4 weeks. Fans maintained positive pressure (controls) and negative pressure (treatment), preventing cross-contamination of enclosures and protecting investigators. Four sets of passive, badge-type samplers were distributed across each enclosure, measuring nitrogen dioxide, sulfur dioxide and volatile organic compounds (NO2, SO2 and VOCs, respectively), and were complemented by active monitors measuring VOCs and particulate matter (2.5 µm diameter, PM2.5). We found that the concentrations of NO2, SO2 and PM2.5 were not different between treatment and control enclosures. Volatile organic compounds (e.g. benzene, toluene, ethylbenzene and xylenes) were approximately six times higher in the treatment enclosure than control (13.23 and 2.13 µg m-1, respectively). In conclusion, this represents a successful, practical design for studying the effects of sub-chronic to chronic exposure to realistic mixtures of vehicle exhaust contaminants, in groups of birds. Recommended modifications for future research include a chassis dynamometer (vehicle treadmill), to better replicate driving conditions including acceleration and deceleration.

Biomarker Sensitivity to Vehicle Exhaust in Experimentally Exposed European Starlings.

The effects of vehicle-related emissions on health has been a long-standing question in human health sciences; however, the toxicology of chronic exposure to environmentally relevant concentrations of these complex mixtures has not been characterized in wild birds. Adult European starlings (Sturnus vulgaris) were exposed to vehicle emissions, with combined benzene, toluene, ethylbenzene, and xylenes (BTEX) concentrations totaling 13.3 µg/m3 over 20 days of exposure for 5 h per day. Exposed birds had significantly lower cell-mediated immunity (measured using phytohaemagglutinin skin test, p < 0.0001), thyroxine (T4, p = 0.042), and glutathione (GSH, p = 0.034) concentrations than control birds. There was no difference in body condition, antibody response to vaccination, triiodothyronine (T3), hepatic biotransformation (7-ethoxyresorufin-O-deethylase activity), or oxidative stress (thiobarbituric acid-reactive substances and ratios of reduced to oxidized GSH) or organ masses between exposed and control birds. This study supports findings of previous studies examining wild birds exposed to these air contaminants and raises concern that environmentally relevant concentrations of common urban volatile pollutants may have measurable effects on health.

European Starlings (Sturnus vulgaris) As Sentinels of Urban Air Pollution: A Comprehensive Approach from Noninvasive to Post Mortem Investigation.

Urban, traffic-related air pollution remains a concern to health-care and environmental professionals, with mounting evidence connecting diverse disease conditions with exposure. Wildlife species such as European starlings (Sturnus vulgaris) cohabit urban neighborhoods and may serve as sentinels for these contaminants. In this novel approach, we use passive, personal-type air samplers to provide site-specific measurements of nitrogen dioxide (NO2), sulfur dioxide (SO2) and volatile organic compounds (VOCs, such as benzene, toluene, ethylbenzene, and xylenes, or BTEX), and account for the effects of confounding environmental factors when teasing out the responses to exposure. This study examines biomarkers of exposure to predominately traffic-related, urban air contaminants in European starlings, including morphometric measurements, immunotoxicology, oxidative stress and hepatic detoxification, and analyses responses in the context of multilayered factors including year, hatch date, weather and location, confirming that this experimental approach and the selected health indicators can be used for comparing locations with different levels of contaminants.

Suspected lead poisoning in two captive cheetahs (Acinonyx jubatus jubatus) in South Africa, in 2008 and 2013.

Whilst lead poisoning in raptors, scavenging birds and waterfowl is well studied and common knowledge, there is surprisingly little literature detailing the risk to mammalian scavengers and captive carnivores fed hunted meat. This case report describes the death of two captive cheetahs (Acinonyx jubatus jubatus) following acute onset of nervous symptoms. Clinical signs included hyper-excitability, seizures, arched back, tail held abnormally high and hyper-salivation. Necropsy findings included bullets or a bullet in their stomachs. Kidney and liver lead levels from one cheetah (15.6 ppm and 17 ppm respectively) were consistent with a diagnosis of lead poisoning; liver from the second cheetah was not available for testing. Both animals were routinely fed hunted antelope or game birds. This is the first report of oral lead poisoning in captive large carnivores, although these are unlikely to be the first cases. Without awareness of the risks of feeding hunted game, lead exposure will continue to be an underdiagnosed reality in the rehabilitation of endangered carnivores.

Sublethal health effects in laboratory rodents from environmentally relevant exposures to oil sands contaminants.

Increasing activity of oil sands extraction and processing in northern Alberta is marked by ongoing controversy about the nature and extent of associated environmental impacts. Bitumen contains a mixture of toxic chemicals, including metals and residual polycyclic aromatic hydrocarbons (PAHs), whose release into the environment poses a distinct risk to the surrounding environment, plus wildlife and human health. In the present study, the authors evaluated several subclinical biomarkers of exposure and effect to mixtures of metals (Pb, Cd, and Hg) and/or PAHs (3 alkylated forms) at environmentally relevant concentrations (100-fold and 10-fold higher than the maximum dissolved concentrations found in snow, to simulate a worst-case scenario), using laboratory mice as a model for future studies of small mammals in the wild. Both metals and alkyl-PAHs exposure were associated with 1) increased relative liver, kidney, and spleen size; 2) alterations in the homeostasis of the antioxidant vitamins A and E in liver; and 3) compromised glutathione redox status in testes, with results also indicating synergistic interactions from co-exposure. The combination of morphometric and oxidative stress biomarkers provide reliable and sensitive measures of the response to contaminant exposure in a mammalian model, suggesting associated physiological costs. Based on the present experimental study, the authors propose that wild small mammals will prove to be valuable sentinel species reflecting sublethal health effects from oil sands-related contaminants. The present study's results also present a basis for the interpretation of future field data.