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1.
Sci Rep ; 13(1): 6769, 2023 04 25.
Article in English | MEDLINE | ID: mdl-37185995

ABSTRACT

Roses are classified as neutral day plants, but high light and cool temperatures produce high quality flowers in roses. As light quantity, the light quality and its special spectra can affect the flower yield and quality. This research aimed to study of the effect of LED light (control (sunlight), blue and red spectra's) and nano-chelated potassium at three levels (0, 1.5 and 3 g/l) on some morphophysiological and biochemical traits of Rosa hybrida cv. Dolce Vita. Light and nano-chelated potassium treatments have a significant effect on most traits measured in the present study. According to the results, the use of red light and nano-chelated potassium in rose cultivation improved the quality characteristics and increased vase life. The highest fresh and dry weight of flowering branch and plant height was observed in red light treatment and the concentration of 3 g/l nano-chelated potassium. Biochemical parameters such as phenolic compounds, leaf and petal flavonoids, petal anthocyanin content, antioxidant capacity and vase life were also significantly increased under red light and with the concentration of 3 g/l nano-chelated potassium compared to the control. In general, it can be said that the use of red light and a concentration of 3 g/l nano-chelated potassium, can be effective in improving the quality of rose flowers, especially in low light condition.


Subject(s)
Rosa , Potassium/pharmacology , Flowers , Plant Leaves , Light
2.
Sci Rep ; 12(1): 16841, 2022 10 07.
Article in English | MEDLINE | ID: mdl-36207586

ABSTRACT

Improving yield and secondary metabolites production of medicinal plants through nutrition management recently has been considered. The present study was done to determine the effects of different ammonium (NH4+) to nitrate (NO3-) ratios (100:0, 75:25, 50:50, 25:75, 0:100) on morphophysiological, nutrient contents (N, P, K, Ca, and Mg), phenolic compounds (Total phenolics (TPC) and flavonoid (TFC) contents and individual phenolics including chlorogenic acid, rosmarinic acid, gallic acid, cinnamic acid, caffeic acid, rutin, p-Coumaric acid, apigenin, and quercetin by HPLC-DAD), essential oil composition (by GC and GC-MS), and antioxidant capacity (by DPPH and FRAP assays) of Moldavian balm (Dracocephalum moldavica L.) in deep water culture (DWC) system. The highest biomass and morphological traits values of D. moldavica observed in 0:100 ratio of NH4+:NO3-. Also, the highest TPC and TFC was earned in plants that supplied with 0:100 ratio of NH4+:NO3-. Using the 25:75 ratio of NH4+:NO3- caused the highest nutrient contents (N, Ca and Mg) in the leaves. p-Coumaric acid was detected as the major abundant phenolic compound in extracts and the application of 75:25 ratio of NH4+:NO3 resulted in the highest amounts of p-Coumaric acid, gallic acid, rosmarinic acid, caffeic acid, quercetin, and rutin. The highest antioxidant capacity by both FRAP and DPPH assays was obtained in 75:25 ratio of NH4+:NO3-. Also, the highest geranial and geranyl acetate, geraniol, and neral were obtained in 75:25, 25:75, and 50:50 ratios of NH4+:NO3-, respectively. Plants supplied with the 0:100 ratio of NH4+:NO3-, had the highest total carotenoids, while the highest chlorophyll a and b content gained with 75:25 ratio of NH4+:NO3-. These results suggest that the management of N source in nutrient recipe could contribute to enhance of morphophysiological traits, antioxidant activity and phytochemical compounds in Moldavian balm.


Subject(s)
Ammonium Compounds , Antipsychotic Agents , Lamiaceae , Oils, Volatile , Ammonium Compounds/metabolism , Antioxidants/analysis , Antioxidants/pharmacology , Apigenin , Caffeic Acids , Carotenoids , Chlorogenic Acid , Chlorophyll A , Cinnamates , Coumaric Acids , Depsides , Flavonoids/pharmacology , Gallic Acid , Lamiaceae/metabolism , Nitrates/metabolism , Phytochemicals/pharmacology , Plant Extracts/pharmacology , Quercetin , Rutin , Water , Rosmarinic Acid
3.
Plants (Basel) ; 10(5)2021 May 17.
Article in English | MEDLINE | ID: mdl-34067603

ABSTRACT

Ethylene is the most important factor playing roles in senescence and deterioration of harvested crops including cut flowers. Brassinosteroids (BRs), as natural phytohormones, have been reported to differently modulate ethylene production and related senescence processes in different crops. This study was carried out to determine the effects of different levels of 24-epibrassinolide (EBL) on ACC oxidase enzyme activity, the final enzyme in ethylene biosynthesis pathway, vase life, and senescence rate in lisianthus cut flowers. Harvested flowers were treated with EBL (at 0, 3, 6, and 9 µmol/L) and kept at 25 °C for 15 days. The ACC oxidase activity, water absorption, malondialdehyde (MDA) production and vase solution absorption rates, chlorophyll and anthocyanin contents, and the vase life of the flowers were evaluated during and at the end of storage. EBL at 3 µmol/L significantly (p ≤ 0.01) enhanced the flower vase life by decreasing the ACC oxidase activity, MDA production and senescence rates, and enhancing chlorophyll and anthocyanin biosynthesis and accumulation, relative water content, and vase solution absorption rates. By increasing the concentration, EBL negatively affected the flower vase life and postharvest quality probably via enhancing the ACC oxidase enzyme activity and subsequent ethylene production. EBL at 6 and 9 µmol/L and in a concentration dependent manner, enhanced the ACC oxidase activity and MDA production rate and decreased chlorophyll and anthocyanin accumulation and water absorption rate. The results indicate that the effects of brassinosteroids on ethylene production and physiology of lisianthus cut flowers is highly dose dependent.

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