A modified sequential wavenumber selection-discriminant analysis with Bayesian optimization strategy for detection and identification of chia seed oil adulteration using Raman spectroscopy.

The detection of oil fraud can be accomplished through the use of Raman spectroscopy, which is a potent analytical technique for identifying the adulteration of edible oils with inferior or less expensive oils. However, appropriate data reduction and classification methods are required to achieve high accuracy and reliability in the analysis of Raman spectra. In this study, data reduction algorithms such as principal component analysis (PCA) and modified sequential wavenumber selection (MSWS) were applied, along with discriminant analysis (DA) as a classifier for detecting oil fraud. The parameters of DA, such as the discriminant type, the amount of regularization, and the linear coefficient threshold, were optimized using Bayesian optimization. The methods were tested on a dataset of chia oil mixed with 5-40 % sunflower oil, which is a common form of fraud in the market. The results showed that MSWS-DA achieved 100 % classification accuracy, while PCA-DA achieved 91.3 % accuracy. Therefore, it was demonstrated that Raman spectroscopy combined with MSWS-DA and Bayesian optimization can effectively detect oil fraud with high accuracy and robustness.

Evaluation of the constituent compounds, antioxidant, anticancer, and antimicrobial potential of Prangos ferulacea plant extract and its effect on Listeria monocytogenes virulence gene expression.

Prangos ferulacea plant is very popular in Iran due to its unique properties in treating diseases and its special flavor. To check the characteristics of this plant, first, its extract was extracted using the maceration method. Its chemical composition was investigated using high-performance liquid chromatography (HPLC) that p-coumaric was identified as its main compound, and Fourier-transform infrared spectroscopy (FTIR) showed the presence of functional groups related to phenolic, flavonoid, tannins, and carboxylic acids such as caffeic acid and coumaric acid composition. Total phenol content (TPC), total flavonoid content (TFC), and beta-carotene were equal to 202.04 ± 5.46 mg gallic acid equivalent (GAE)/g dry weight, 1,909.46 ± 13 µg quercetin (QE)/g of dry weight, and 2.91 mg/100 g. The antioxidant property of the extract was evaluated using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS) free radical scavenging and ferric reducing antioxidant power assay (FRAP). According to the IC50 obtained for DDPH (274 ± 7.2 µg/mL) and ABTS (120.45 ± 9.6 µg/mL) and FRAP values [1.92 ± 0.05 µg ascorbic acid equivalent (AAE)/g of extract], this extract had high antioxidant properties. Cytotoxicity was evaluated against the survival of HT 29 cells that IC50 was 82.15 ± 0.02 µg/mL. The antimicrobial property of the extract was calculated using disk diffusion agar (DDA), well diffusion agar (WDA), minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC). Listeria monocytogenes has the highest sensitivity to this extract and inhibition zone based on DDA and WDA method and with an MIC and MBC equal to 16 and 128 mg/mL has the least resistance. The morphology change of L. monocytogenes strain was proved through scanning electron microscope (SEM) and confocal laser scanning microscopy (CLSM). The extract caused a significant reduction in the transcription of genes involved in the film formation ability of L. monocytogenes. The obtained results fully prove the very practical and pragmatic characteristics of P. ferulacea.

Development of an Innovative Optoelectronic Nose for Detecting Adulteration in Quince Seed Oil.

In this study, an innovative odor imaging system capable of detecting adulteration in quince seed edible oils mixed with sunflower oil and sesame oil based on their volatile organic compound (VOC) profiles was developed. The system comprises a colorimetric sensor array (CSA), a data acquisition unit, and a machine learning algorithm for identifying adulterants. The CSA was created using a method that involves applying a mixture of six different pH indicators (methyl violet, chlorophenol red, Nile blue, methyl orange, alizarin, cresol red) onto a Thin Layer Chromatography (TLC) silica gel plate. Subsequently, difference maps were generated by subtracting the "initial" image from the "final" image, with the resulting color changes being converted into digital data, which were then further analyzed using Principal Component Analysis (PCA). Following this, a Support Vector Machine was employed to scrutinize quince seed oil that had been adulterated with varying proportions of sunflower oil and sesame oil. The classifier was progressively supplied with an increasing number of principal components (PCs), starting from one and incrementally increasing up to five. Each time, the classifier was optimized to determine the hyperparameters utilizing a random search algorithm. With one to five PCs, the classification error accounted for a range of 37.18% to 1.29%. According to the results, this novel system is simple, cost-effective, and has potential applications in food quality control and consumer protection.

Effects of aerosolized citric acid-radio frequency as a pretreatment on hot-air drying characteristics of banana.

The effects of aerosolized citric acid-radio frequency (RF) pretreatment were evaluated on the quality characteristics of hot air-dried banana. The results showed that increasing the RF intensity elevated the total phenolic content (TPC), shrinkage, and color changes, while the TPC and color changes decreased with increasing the RF exposure duration. A rise in the RF intensity reduced the rehydration ratio (RR) and firmness of the samples. Aerosolization of citric acid rendered the preservation of the phenolic compounds of the samples to a higher extent, and TPC decreased from 311 ± 3.4 mg/g in fresh banana to 252.1 ± 4.24 mg/g in the samples treated with a RF of 27.12 Hz for 40 min, 280.5 ± 8.1 mg/g in the ones treated with 1% aerosolized citric acid for 40 min, and 162.5 ± 10.8 mg/g in the ones with no pretreatment. According to scanning electron microscopy (SEM), the application of aerosolized citric acid pretreatment caused tissue softening and the formation of cell holes in the samples. Cell wall collapse and damage were severe when RF was in use, which caused the blockage of some microchannels within the tissue. The Page model with the highest determination coefficient (R 2) and the lowest root-mean-squared error (RMSE) and chi-square (χ 2) was selected as the best model.

Effect of chia seed mucilage/bacterial cellulose edible coating on bioactive compounds and antioxidant activity of strawberries during cold storage.

This study aimed to investigate the effect of chia seed mucilage (CSM) - bacterial cellulose nano-fiber (CNF) edible coating on bioactive compounds and antioxidant enzyme activity of strawberries. Strawberries were coated with CSM containing 0.6 and 8.0% (w/w) of CNF. The content of total phenol, flavonoids, anthocyanin, ascorbic acid, protein content, antioxidant activity and the activity of polyphenol oxidase, peroxidase, superoxide dismutase and phenylalanine ammonia-lyase enzymes were evaluated. The use of CSM - CNF edible coatings further preserved the phenolic, flavonoid, ascorbic acid and antioxidant activity of strawberries, and this effect was more evident in the CSM-coated sample containing CNF; However, the accumulation of anthocyanins in the coated samples was lower than the control sample. The activity of polyphenol oxidase and peroxidase enzymes, which lead to the degradation of phenolic compounds and brown color in the product, was also effectively controlled by the edible coating.

Utilization of Plantago major seed mucilage containing Citrus limon essential oil as an edible coating to improve shelf-life of buffalo meat under refrigeration conditions.

This study is aimed to develop a novel edible coating based on Plantago major seed mucilage (PMSM) and Citrus limon essential oil (CLEO) to increase the shelf-life of buffalo meat during cold storage. The CLEO was firstly isolated by the hydrodistillation method, and it contained mainly limonene (40.5%) and carene (15.4%) with remarkable antioxidant activity (55.7%, 63.8%, and 51.85% based on the DPPH-radical scavenging, ABTS-radical scavenging, and carotene-linoleic acid bleaching tests, respectively) and antibacterial effect against some pathogenic and spoilage microorganisms. The CLEO (0%, 0.5%, 1%, 1.5%, and 2%) was then incorporated into PMSM solution to develop a novel CLEO-loaded PMSM edible coating for improving the shelf-life of buffalo meat. The edible coating was able to significantly reduce the progression of lipid oxidation (peroxide value) and microbial growth (total viable count, psychrotrophic bacteria, Escherichia coli, Staphylococcus aureus, and fungi) in buffalo meat during storage period of 10 days at 4°C in comparison with the control (noncoated sample). The meat hardness and sensory properties (i.e., odor, color, appearance, texture, and overall acceptability) were also maintained better upon edible coating applications. Based on the results, the CLEO-rich PMSM edible coating could be applied as a new and effective preservative to improve the stability of meat products to lipid oxidation and microbial spoilage.

The impact of Qodume Shirazi seed mucilage-based edible coating containing lavender essential oil on the quality enhancement and shelf life improvement of fresh ostrich meat: An experimental and modeling study.

Today, food consumers prefer to use the foods that contain natural preservatives. The purpose of this study was to investigate the effect of Qodume Shirazi seed mucilage (QSSM) and lavender essential oil (LO) on the preservation of ostrich meat during cold storage. The chemical compounds of LO were identified through gas chromatography-mass spectrometry (GC/MS). The ostrich meat samples were coated with the mucilage containing the essential oil at concentrations of 0%, 0.5%, 1%, 1.5%, and 2%, v/v. The control and the coated ostrich meat samples were kept at 4°C and analyzed for microbiological (total viable count, psychrotrophic count, Escherichia coli, Staphylococcus aureus, coliforms, and fungi), physicochemical (moisture content, pH, texture, and color parameters), and sensorial (odor, color, and total acceptance) characteristics during 9 days of storage. GC/MS identified 12 compounds in the essential oil, among which linalool was the major one (43.3%). The lightness (L* value) and hardness of all the ostrich meat samples were reduced during the storage. From a microbiological point of view, the cold storage duration for the control and the coated sample without the essential oil was only 3 days, while for coated samples containing 0.5%, 1%, 1.5%, and 2% essential oil, it was 3, 3, 6, and 9 days, respectively. The coated ostrich meat containing 2% LO had an appropriate quality with an expanded shelf life. The results showed that neural network with 10 neurons in the hidden layer had the lowest mean squared error and mean absolute error and the highest correlation coefficient for predicting the quality and microbial properties of the coated meat samples during storage.

Electrospun chia seed mucilage/PVA encapsulated with green cardamonmum essential oils: Antioxidant and antibacterial property.

In this work, the potential of chia seed mucilage (CSM) as a new source of carbohydrate for encapsulation of green cardamonmum Essential oils (GCEOs) was evaluated. 1H NMR spectrum, FTIR spectrum and, SEM image has confirmed the existence of the GCEOs in the nanofibers. The nanofibers of CSM and polyvinyl alcohol have not antibacterial property, while nanofibers containing GCEOs show antibacterial activity against E. coli and S. aureus. Incorporating GCEOs in CSM nanofibers improved the antioxidant of the generated nanofibers. The amount of radical scavenging for the nanofibers containing 16 (mg/ml) of GCEOs was 18% and increasing the GCEOs concentration up to 64 (mg/ml) leads to grow the activity up to 41%. Thus, our studies indicate that nanofiber can be used as a novel antioxidant and antibacterial agent in the food and pharmaceutical industry.

Indoor multiuser visible light communication systems using Hadamard-coded modulation.

Visible light communications (VLC) is a short-range optical wireless communication technology that uses light-emitting diodes (LEDs) as lighting devices and data transmitters. This paper describes a multiuser VLC system using Hadamard-coded modulation (HCM) for indoor data transmission. Considering the peak transmitted power limit of the LEDs, a DC-reduced HCM (DCR-HCM) is used to reduce the nonlinear clipping distortion. Since the Hadamard codewords have different bandwidth requirements for a given symbol rate, they can be assigned to users with varying hardware capabilities. Optimally assigning codewords to users is found to significantly improve the average throughput, up to twice higher than a random assignment for a typical scenario. When the number of active users is less than the size of the Hadamard matrix used, more than one codeword can be assigned per user, which further improves the throughput. This paper also examines a scenario where multiple lamps in an indoor space transmit the same data. Since the time of arrival for the received signals emitted from different lamps is different, the Hadamard codes received are no longer orthogonal, resulting in multiple access interference and inter-chip interference. The number of acceptable codewords is computed based on the specific interference experienced in different parts of the indoor space. The spatial distribution of the maximum throughput is also simulated, showing that the ratio of the maximum to the minimum data rate can be as high as 10 when considering the entire area of a typical indoor room. This article is part of the theme issue 'Optical wireless communication'.

Cumin essential oil: Phytochemical analysis, antimicrobial activity and investigation of its mechanism of action through scanning electron microscopy.

In this study, the antimicrobial effects of cumin essential oil (CEO) and its mechanism of action through scanning electron microscopy (SEM) against Escherichia coli and Listeria innocua were investigated. The SEM images were taken at 0, 12 and 24 h at the minimum inhibitory concentration (MIC). The chemical composition of CEO was identified through gas chromatography/mass spectrometry (GC-MS). The antimicrobial effects of CEO were evaluated by the methods of Kirby-Bauer, well diffusion agar, microdilution broth and minimum bactericidal/fungicidal concentration (MBC/MFC). Antioxidant activity was examined by the methods of ß-carotene/linoleic acid inhibition and 2,2-diphenyl-1-picrylhydrazyl. Total phenol content (TPC) was measured by Folin-Ciocalteu method. The subsequent analysis of CEO through GC-MS revealed that cuminal (28.28%) was the major compound of CEO. CEO showed a high TPC of 89.45 ±â€¯0.78 mg GAE/g. The free radical scavenging activity of CEO (based on IC50) was equal to 9.10 ±â€¯0.63 µg mL-1. In addition, CEO showed a remarkably high inhibitory effect (63%) on ß-carotene bleaching via neutralizing hydroperoxides, which are responsible for the oxidation of highly unsaturated ß-carotene. The antimicrobial effect increased as a function of essential oil concentration. However, there were no inhibitory effects on E. coli at 5 mg mL-1. The electron micrographs demonstrated that CEO caused an increase in the permeabilization of the cells and disrupted the membrane integrity.

Inhibition of Escherichia coli adhesion to human intestinal Caco-2 cells by probiotic candidate Lactobacillus plantarum strain L15.

Probiotics are microbial strains beneficial to human health if consumed in appropriate amounts. Their potential has recently led to a significant increase in research interest in their effects on the intestine, mainly by reinforcing the intestinal epithelium and modulating the gut microbiota. This study aimed to evaluate the probiotic features of Lactobacillus plantarum strain L15 based on adhesive properties for the inhibition of the adhesion of infectious pathogens. The molecular identification of the strain was performed from the sequencing of 16S ribosomal DNA with 27FYM and 1492R primers, and its probiotic features, including resistance to gastric juices, resistance to bile salts, and hydrophobicity were evaluated. The potential of Lactobacillus plantarum strain L15 to adhere to human adenocarcinoma intestinal cell line, Caco-2, as well as the auto and co-aggregation and anti-adherence activity against Escherichia coli were investigated. The results demonstrated that this strain has a desirable potential for passing through the low pH of the stomach and entering the intestines. Moreover, 54% hydrophobicity, 44% auto-aggregation, and 32% co-aggregation were observed for this strain. The adhesion level of Lactobacillus plantarum strain L15 to Caco-2 cells was 12%, and adhered lactobacilli cells were observed by scanning electron microscopy (SEM). Furthermore, this strain showed appropriate anti-adherence effects, including competition, inhibition, and replacement properties against Escherichia coli. The results indicated that Lactobacillus plantarum strain L15 had good potential for exerting antagonistic effects against E. coli.

The preparation, characterization and in vitro application evaluation of soluble soybean polysaccharide films incorporated with cinnamon essential oil nanoemulsions.

In this study, we developed the new bioactive film from soluble soybean polysaccharide (SSPS) incorporated with different concentrations of cinnamon essential oil nanoemulsions (CNO) and the functional properties of them were evaluated. Then CNO-SSPS film was applied on the meat during refrigerate for 8 days. The use of CNO in film production has reduced thickness, water vapor permeability, water solubility, lightness (L*), redness (a*) and whiteness (WI) and increased antioxidant activity of SSPS-films. Also, the SSPS- film containing 0.6% CNO activity only on gram-positive bacteria (Staphylococcus aureus and Streptococcus pyogenes) and SSPS- film containing 0.8% CNO had antimicrobial activity on gram-positive and gram-negative bacteria. In vitro application, the pH of the meat treated with CON remained in the natural pH of meat during storage (8 days). Based on the results, the highest and lowest hardness values were for the samples of CNO- 0.8 and control, respectively. Incorporating CON at 0.6 and 0.8% concentration of cinnamon reduced 4.14 and 5.71 log cycle in the total aerobic viable count compared to uncoated and decreased of yeast and molds on 8th day by 1 log cycle compared uncoated. These resulted showed CNO-SSPS film can be used as a good preservative in meat products.

Black Zira essential oil: Chemical compositions and antimicrobial activity against the growth of some pathogenic strain causing infection.

The aim of this study was to perform chemical compositions and phytochemical analysis of Black Zira essential oil and other goal of this research was to investigate the antimicrobial effects of Black Zira essential oil against Enterobacter aerogenes, Pseudomonas aeruginosa, Escherichia coli, Shigella flexneri, Staphylococcus epidermidis, Streptococcus pyogenes and Candida albicans. Black Zira essential oil was extracted by hydrodistillation method using clevenger apparatus. Black Zira essential oil chemical composition was identified through gas chromatography/mass spectrometry. γ-terpinene with a percentage of 24.8% was the major compound of Black Zira essential oil. The antimicrobial effect Black Zira essential oil was evaluated by several qualitative and quantitative methods (disk diffusion, well diffusion, microdilution broth, agar dilution and minimum bactericidal/fungicidal concentration). Phytochemical analysis Black Zira essential oil were appraised based on qualitative methods. Antioxidant activity (2,2-diphenyl-1-picrylhydrazyl and ß-carotene/linoleic acid inhibition) and total phenolic content (Folin-Ciocalteu) were examined. The results of phytochemical analysis of Black Zira essential oil showed the existence of phenolic, flavonoids, saponins, alkaloids and tannins. The total phenolic content and antioxidant activity (reported as IC50) of Black Zira essential oil were equal to 120.50 ±â€¯0.50 mg GAE/g and 11.55 ±â€¯0.25 µg/ml, respectively. The MIC of the Black Zira essential oil ranged from 1 mg/ml to 8 mg/ml, while its MBC and MFC ranged from 1 mg/ml to 16 mg/ml. The results presented that the longest and the shortest inhibition zone diameter at the concentration of 8 mg/ml pertained to C. albicans and E. aerogenes, respectively.

Modeling of ultrasound-assisted extraction, characterization and in vitro pharmacological potential of polysaccharides from Vaccinium arctostaphylos L.

In the study, Box-Behnken design was used to optimized the operational parameters involved in ultrasound-assisted extraction (UAE) of Vaccinium arctostaphylos polysaccharide (VAP) including ultrasound power, extraction temperature and time and water to raw material ratio. The highest extraction polysaccharide was obtained at 100W, 19.12min, 79.32C and 20 (ml water/g powdered Vaccinium arctostaphylos). Under these condition, total anthocyanin, total phenolic contents and total flavonoid were 10.16mlg/g, 0.43mlg/g and 0.58mlg/g, respectably. Also, structure of polysaccharide was identified by GC-Mass Fourie transform-infrared spectroscopy (FT-IR). Then, we demonstrated that VAPs had significantly scavenging activities against 2,2-diphenyl-1-picrylhydrazyl and hydroxyl radicals in vitro. The effect of degradation of DPPH was increased by increasing the concentration of polysaccharide up to 15mg/ml. The anti-microbial activity of the resulted polysaccharide with highest concentration (15mg/ml) was the following in order of inhibition zone diameter: B. panis (9.89mm)>S. aureus (9.12mm)>Sh. dysenteriae (7.45mm)>E. coli (6.38mm). VAPs can thus be used as anexcellent antioxidant and antimicrobial ingredient in food and medicinal preparations.

Characterization of Hypericum perforatum polysaccharides with antioxidant and antimicrobial activities: Optimization based statistical modeling.

In this study, the extracting parameters of crude polysaccharides (CPSs) from the Hypericum perforatum (HP) including extraction time (ETi, 60-180min), extraction temperature (ETe, 60-90°C), and the water/solid ratio (W/S, 10-30 was optimized by using three-variable-three-level Box-Behnken design-response surface methodology (BBD-RSM) based on the single-factor experiments. The optimal extraction conditions were as follow: ETi 117.5min, ETe 74.28°C, and W/S 20.3:1. Under these conditions, the experimental yield was 6.69%. Fourier transform-infrared spectroscopy (FT-IR) was used to identify structure of polysaccharide extracted at the optimal operating point. HP-CPSs was proved to possess antioxidant activities including 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and OH free-radicals scavenging activates in vitro. The antibacterial activities of HP-CPSs against Escherichia coli, Shigella dysenteriae and Salmonella typhi, Bacillus cereus and Staphylococcus aureus were evaluated by determining clear growth inhibition zone diameters and by essays in liquid media. Overall, the results indicated that those polysaccharides could offer promising sources of polysaccharides for future application as antioxidant ingredients in the food industry.

Polysaccharide of caper (Capparis spinosa L.) Leaf: Extraction optimization, antioxidant potential and antimicrobial activity.

Three-variable-three-level Box-Behnken design-response surface methodology (BBD-RSM) based on the single-factor experiments was used to optimize the extracting parameters of crude polysaccharides (CPSs) from the Capparis spinosa leaves (CSL) including extraction time (ETi, 60-120min), extraction temperature (ETe, 60-80°C), and the water/solid ratio (W/S, 6-16). The optimal process conditions in order to the highest yield (6.73%) of CSL-CPSs were 119.8min ETi, 72.84°C ETe, and 15.97:1W/S.Structure of polysaccharide extracted at the optimal operating point were identified by Fourier transform-infrared spectroscopy (FT-IR). CSL-CPSs (50-300µg/L) revealed significantly scavenging activities against 2,2-diphenyl-1-picrylhydrazyl (DPPH)and OH free-radicals in vitro. A much more antimicrobial activity using this polysaccharide against Gram-negative bacteria (Escherichia coli, Shigella dysenteriaeandSalmonella typhi) was found than Gram-positive bacteria (Bacillus panis and Staphylococcus aureus). CSL-CPSs can thus be used as anexcellent antioxidant and antimicrobial ingredient in food and medicinal preparations.

Complementary lattice arrays for coded aperture imaging.

In this work, we propose the concept of complementary lattice arrays in order to enable a broader range of designs for coded aperture imaging systems. We provide a general framework and methods that generate richer and more flexible designs compared to the existing techniques. Besides this, we review and interpret the state-of-the-art uniformly redundant array designs, broaden the related concepts, and propose new design methods.

Effect of layer-by-layer polyelectrolyte method on encapsulation of vanillin.

The objective of this work was to microencapsulate vanillin by multilayer emulsion followed by spray drying, aiming to protect it and control its release. An electrostatic layer-by-layer deposition method was used to create the multilayered interfacial membranes around microcapsules with different compositions: (i) one-layer (soy protein isolate); (ii) two-layer (soy protein isolate - OSA starch); (iii) three-layer (soy protein isolate - OSA starch - Chitosan). The morphology of the microcapsules was analyzed by scanning electronic microscopy. The hygroscopicity, solubility, particle size, encapsulation efficiency, Fourier transform infrared spectroscopy and release into water (37°C and 80°C) were also examined. FTIR confirmed the interaction between the wall materials. All microcapsules were not very water-soluble or hygroscopic while three-layer microcapsules compared to one and two layer microcapsules have lower moisture content and predominantly shriveled surfaces. The results indicated it was possible to encapsulate vanillin with the techniques employed and that these protected the vanillin even at 80°C. The reduced solubility and low release rates indicated the enormous potential of the vehicle developed in controlling the release of the vanillin into the food and pharmaceuticals.