[The study on the impact of various types of currents on root canal microbiota]. / Izuchenie vliianiia raznykh vidov transkanal'nogo vozdeistviia postoiannym tokom na mikrofloru kornevykh kanalov.

THE PURPOSE OF THE STUDY: to evaluate the effect of different types of direct current transcanal effects on the root canal microbiota. The study involved 91 volunteers aged 18 to 65 years, in 48 (53%) no periapical lesions were detected, in 43 (47%) periapical lesions. For determination of the optimal parameters of dosage an apex-phoresis, that provides antibacterial effect, clinical strains the facultative anaerobic of bacteria was used, after collection from root canal of teeth. The most pronounced antimicrobial action in which an electrode placed in the cavity of the tooth is connected to the plus of a current source - are transcanal anodalgvanization and apex-phoresis. For transcanal anodalvanization, these are copper ions, and for apex-foreses, a combination of silver ions and copper ions. Both methods showed high antibacterial activity against all representatives of anaerobic microbiota. The obtained data are confirmed with a molecular and genetic method of identification of the hardly cultivated virulent anaerobic bacteria using the diagnostic kit for PCR 'Multident-5'.

[The Physiotherapy in the treatment of alveolitis and localized osteomyelitis of the jawbones]. / Fizioterapiia pri lechenii al'veolita i ogranichennogo osteomielita cheliustei.

BACKGROUND: Alveolitis and localized osteomyelitis of the jawbones still remain the most common conditions among the post-operative complications associated with tooth extraction. AIM: The objective of the present study was to evaluate the antibacterial and clinical effectiveness of ozone therapy and radiation of various wavelengths emitted by the light-emitting diodes (LED) applied for the treatment of alveolitis and localized osteomyelitis of the jawbone with a view to improving the effectiveness of the treatment and shortening the duration of the rehabilitation period. MATERIAL AND METHODS: A total of 100 patients presenting with alveolitis and localized osteomyelitis of the jawbones at the age varying from 18 to 65 years have been examined and treated. All the patients were divided into four equal groups. The first group (1) was comprised of the patients who were treated with the combination of the measures which included the course of ozone therapy and LED-emitted red light radiation (630 nm); the patients of the second group (2) were treated using ozone therapy in the combination with LED-emitted green light radiation (530 nm); in the third group (3), ozone therapy in the combination with LED-emitted (470 nm) blue light radiation was used. The patients of the fourth group (4) were managed without the application of the physiotherapeutic factors. To study the effects of ozone therapy and LED-emitted radiation of different wavelengths on the microflora of the extracted tooth socket, the bacteriological analysis was carried out three times - before the treatment, within three and five days after its initiation. RESULTS: The present study has demonstrated that the combination of ozone therapy with LED-emitted (630 nm) red light radiation provides the most efficient tool for the treatment of alveolitis whereas ozone therapy in the combination with LED-emitted (470 nm) blue light radiation should be preferred for the treatment of localized osteomyelitis of the jawbone. CONCLUSIONS: The results of this study are of primary importance as giving evidence of the significant reduction in the duration of the rehabilitation period in the patients presenting with alveolitis and localized osteomyelitis of the jawbones.

Clinical-microbiological research of action ozone therapy and light-emetting diode radiation of red range (630 nanometers) on microflora of the hole extracted toothatalveolitis and limited osteomyelitis of jaws. / Kliniko-mikrobiologicheskoe issledovanie deistviya ozonoterapii i svetodiodnogo izlucheniya krasnogo diapazona (630 nm) na mikrofloru lunki udalennogo zuba pri al'veolite i ogranichennom osteomielite chelyustei.

As a result of cliniko-microbiological research the data testifying to substantial improvement of efficiency of antimicrobictherape at inclusion in a complex of medical actions at alveolitis and the limited osteomyelitis of a jow ozone therapy in a combination with a light-emettinf diode irradiation of the hole extracted teeth red ( 630 nanometers) are obtained by light.

[Evaluation of antibacterial activity of apex-phoresis].

Effects of apex-phoresis on dental root canal microflora were studied and optimal dosage parameters were determined. Microbiological study in vitro has established that optimal antibacterial effect was achieved with the apex-phoresis dosage in the range between 2.5-5 mA/min. Study of the effects of apex-phoresis on dental root canal microflora in vivo disclosed its high antibacterial activity both in cases of pulpitis and destructive forms of chronic periodontitis. For the first time the data are confirmed by molecular-genetic method of detection of hard to cultivate virulent anaerobic bacteria. The results of the study allow to recommend apex-phoresis for wide use in clinical practice as an effective way to sterilize difficult to traverse dental root canals.

[Use of molecular genetic systems for diagnostics of inflammatory diseases of oral mucosa and periodontium].

Structure of bacterial microflora of periodontal pocket using classical cultural and molecular genetic methods (PCR and RT-PCR assays) was studied in patients with chronic generalized severe or intermediate periodontitis in acute phase and in healthy subjects. Structure of viral and fungal microflora using PCR or ELISA assays was studied also. Markers of Gram-negative bacteria (B. forsithus, T. denticola, A. actinomicetemcommitans, P. intermedia and P. gingivalis) were detected with different rates. Rate of detection of pigment-forming P. intermedia and P. gingivalis was significantly higher when PCR assay was used compared with traditional bacteriologic method. Detection rate of Candida albicans DNA in tooth-gingival fluid was virtually not different from one when cultural method was used. PCR assay is recommended as laboratory method for detection of DNA of microorganisms associated with inflammatory diseases of oral mucosa and periodontium.