1.
Mol Cell
; 5(1): 85-95, 2000 Jan.
Article
in English
| MEDLINE
| ID: mdl-10678171
ABSTRACT
Passage through mitosis is required to reset replication origins for the subsequent S phase. During mitosis, a series of biochemical reactions involving cyclin-dependent kinases (CDKs), the anaphase promoting complex or cyclosome (APC/C), and a mitotic exit network including Cdc5, 14, and 15 coordinates the proper separation and segregation of sister chromatids. Here we show that cyclin B/CDK inactivation can drive origin resetting in either early S phase or mitosis. This origin resetting occurs efficiently in the absence of APC/C function and mitotic exit network function. We conclude that CDK inactivation is the single essential event in mitosis required to allow pre-RC assembly for the next cell cycle.
Subject(s)
Cell Cycle/physiology , Cyclin-Dependent Kinases/metabolism , Ligases/physiology , Mitosis/physiology , Protein Tyrosine Phosphatases , Saccharomyces cerevisiae Proteins , Saccharomyces cerevisiae/cytology , Saccharomyces cerevisiae/genetics , Ubiquitin-Protein Ligase Complexes , Anaphase-Promoting Complex-Cyclosome , CDC28 Protein Kinase, S cerevisiae/metabolism , Cell Cycle Proteins/metabolism , Chromatids/physiology , Fungal Proteins/metabolism , GTP-Binding Proteins/metabolism , Genotype , RNA-Binding Proteins , S Phase , Ubiquitin-Protein Ligases
2.
Cold Spring Harb Symp Quant Biol
; 65: 333-42, 2000.
Article
in English
| MEDLINE
| ID: mdl-12760047