ABSTRACT
To check the ability of the anticancer activity, the extracted extracellular polymeric substances (EPS) from marine actinobacteria were done. Potential of the anticancer activity of EPS which was extracted from marine actinobacteria of Streptomyces species through an assay called MTT. Marine actinobacterial isolation, identification and micromorphology of the strain, and biochemical analysis were performed (Shirling and Gottlieb, 1966). The production of EPS from marine actinobacteria was quantified(P. Sivaperumal et al., 2018). Carbohydrate content in the EPS was quantified, and MCF-7 cell proliferation was done using an MTT assay. EPS-producing marine Streptomyces was isolated and identified. The production of EPS and their protein, carbohydrate, lipid, and other parameters were estimated. Further, the EPS showed more than 50% of inhibition after 72 h using the MTT assay in the MCF-7 cancer cell line. The present study exhibited that EPS from marine Streptomyces species has significant anticancer activity.
ABSTRACT
Tooth discoloration is considered as one of the most common dental problems among people, and in dentistry, the most common cases are claimed after the restoration. Therefore, in this research, we aim to evaluate the effect of carbonated beverages on the color stability of bulk and flowable composite resin. For the study, 12 composite disc samples were made using the standard dimensions of 10 mm diameter and 2 mm thick. To find the color stability, we used a VITA Easyshade spectrophotometer. We used two different composites of bulk fill and flowable composite resin; the composite brand we used was Tetric ecom plus; as an immersion medium, we used two different carbonated beverages, and the chosen beverages were Appy Fizz and 7Up. 24-h and 7-day postimmersion color stability was evaluated. In the results of postimmersion, we have found the Delta E value for 24 h immersion of flowable and bulk fill composite as 5.8115 and 7.4378, respectively; similarly, the Delta E value for 7 days immersion of flowable and bulk fill composite was 9.9559 and 10.1028, respectively. Using the independent "t"-test, we found that the significance is 0.633 and 0.328, which was statistically not significant. In the present study, when immersed in Appy Fizz juice and 7Up juice, bulk fill composite resins have shown greater discoloration when compared to flowable composite resin material. Thus, the flowable composite resin samples were more color stable.
