The Incidence of Amyotrophic Lateral Sclerosis in Ohio 2016-2018: The Ohio Population-Based ALS Registry.

INTRODUCTION: Amyotrophic lateral sclerosis (ALS) is a fatal, neuromuscular disease with no cure. ALS incidence rates have not been assessed specifically in Ohio, yet the state contains both metropolitan and rural areas with a variety of environmental factors that could contribute to disease etiology. We report the incidence of ALS in Ohio residents diagnosed from October 2016 through September 2018. METHODS: We engaged practitioners from 9 Ohio sites to identify newly diagnosed ALS patients and to complete case report forms with demographic and clinical information. ALS was diagnosed according to the Awaji criteria and classified as either definite, probable, or possible. We developed a method to estimate missing cases using a Poisson regression model to impute cases in counties with evidence of undercounting. RESULTS: We identified 333 newly diagnosed ALS patients residing in Ohio during the 2-year index period and found incidence rates varied in the 88 state counties. After incorporating the estimated 27% of missing cases, the corrected crude annual incidence was 1.96/100,000 person-years, and the age- and gender-standardized incidence was 1.71/100,000 person-years (standardized to the 2010 US census). DISCUSSION/CONCLUSION: The estimated Ohio incidence of ALS is overall similar to that reported in other states in the USA. This study reveals a geospatial variation in incidence within the state, and areas with higher rates warrant future investigation.

The inhibitory impact of ammonia on thermally hydrolyzed sludge fed anaerobic digestion.

This study evaluated the impact of ammonia on mesophilic anaerobic digestion (AD) with thermal hydrolysis pretreatment (THP) treating a mixture of primary sludge and waste activated sludge and operated under constant organic loading rate of 9 kg COD/m3 /d. Free ammonia concentrations in the digesters were varied between 37 and 966 mg NH3 -N/L, while maintaining all other operational conditions constant. A decrease in volatile solids reduction from 54 ± 5% (at <554 mg NH3 -N/L) to 35 ± 6% at the maximum free ammonia concentration of 966 mg NH3 -N/L was observed at steady-state conditions. No impact of free ammonia on final dewaterability was detected. Free ammonia thus mostly limited methanogenesis. A free ammonia Monod inhibition constant of 847 ± 222 mg NH3 -N/L for methanogens was estimated based on the digester steady-state methane rates dynamics. This study showed that current THP AD digesters (typically 110-260 mg NH3 -N/L) operate under 12%-18% ammonia inhibition for methanogenesis. Operation under SRT of 15 days, about 2 times more than needed to retain methanogens, can compensate for lower methanogens rates and avoid performance impacts. The later shows a good potential to operate under higher free and total ammonia concentration without jeopardizing performance. PRACTITIONER POINTS: Only from a free ammonia concentration above 554 mg NH3 -N/L, decreased volatile solids reduction and biogas yield were observed. A volatile solids reduction of 35 ± 6% at maximum free ammonia concentration of 966 mg NH3 -N/L was still achieved. A Monod inhibition constant for methanogens of 847 ± 222 mg NH3 -N/L was estimated. It was estimated that current THP AD systems (110-260 mg NH3 -N/L) operate under 12%-18% NH3 inhibition for methanogenesis.

Risk factors for amyotrophic lateral sclerosis: A regional United States case-control study.

Most amyotrophic lateral sclerosis (ALS) cases are considered sporadic, without a known genetic basis, and environmental exposures are thought to play a causal role. To learn more about sporadic ALS etiology, we recruited n = 188 ALS patients from northern New England and Ohio and matched controls 2:1 from the general population of the same regions. Questionnaires evaluated the association between a variety of lifestyle, behavioral (ie, hobbies and activities), and occupational factors and the risk of ALS, including the duration of time between exposure and ALS onset, and exposure frequency. Head trauma was associated with increased ALS risk (adjusted odds ratio [OR] 1.60 95% confidence interval [CI] 1.04-2.45), with significantly greater effects for injuries occurring 10 or more years prior to symptom onset (P = .037). ALS risk was increased for those reporting severe electrical burns (adjusted OR 2.86, 95% CI 1.37-6.03), with odds ratios highest for burns after age 30 (OR 3.14), and for burns 10 or more years prior to symptom onset (OR 3.09). Hobbies involving lead were the most strongly associated with ALS risk (adjusted OR 2.92, 95% CI 1.45-5.91). Exposures to lead 20 or more years prior to diagnosis had larger effect sizes compared to those occurring more recently. Holding a job in mechanics, painting, or construction was associated with ALS. The identification of these specific environmental factors associated with ALS highlight the need for future prospective and laboratory studies to assess causality, biological mechanisms, and find prevention or treatment opportunities.

Increasing oxygen transfer efficiency through sorption enhancing strategies.

The link between aeration efficiency and biosorption capacity in water resource recovery facilities was extensively investigated, with special emphasis on wastewater characteristics and the development of strategies to maximize adsorption. Biosorption of oxygen transfer inhibitors (i.e., surfactants, colloidal, and soluble fractions) was examined by a series of pilot batch-scale experiments and full-scale studies. The impact of a sorption-enhancing strategy (i.e., bioaugmentation) deployed at full-scale over a five-year period was evaluated. Bench-scale experiments determined the inhibition coefficient (Ki) to measure the impact of surfactants and COD fractions as inhibitors of oxygen transfer efficiencies (αSOTE) in wastewater systems. The inhibition constant for surfactants Ki was found at 2.4 ± 0.4 mg L-1 SDS while for colloidal material was at 14 ± 1 mg L-1 (no inhibition for soluble fraction was found). Two enhancing biosorption configurations (i.e., contact stabilization and anaerobic selector) resulted in significant improvements in both aeration efficiency indicators (αSOTE) and surfactants removals. αSOTE improvements of 46% and 54% in comparison to conventional high rate activated sludge process (HRAS) were reported. Similarly, the removal of surfactants was increased by 27% and 56% using optimized enhancing-sorption strategies. Further analyses helped elucidate the underlying mechanisms of surfactants removal. Findings are expected to help full-scale applications increase their sorption potential as well as the concurrent aeration efficiency, which helps WRRFs to advance toward energy-positive wastewater treatments.

Salmonellosis Outbreak Detected by Automated Spatiotemporal Analysis - New York City, May-June 2019.

In May 2019, the New York City Department of Health and Mental Hygiene (NYCDOHMH) detected an unusual cluster of five salmonellosis patients via automated spatiotemporal analysis of notifiable diseases using free SaTScan software (1). Within 1 day of cluster detection, graduate student interviewers determined that three of the patients had eaten prepared food from the same grocery store (establishment A) located inside the cluster area. NYCDOHMH initiated an investigation to identify additional cases, establish the cause, and provide control recommendations. Overall, 15 New York City (NYC) residents with laboratory-diagnosed salmonellosis who reported eating food from establishment A were identified. The most commonly consumed food item was chicken, reported by 10 patients. All 11 clinical isolates available were serotyped as Salmonella Blockley, sequenced, and analyzed by core genome multilocus sequence typing; isolates had a median difference of zero alleles. Environmental assessments revealed food not held at the proper temperature, food not cooled properly, and potential cross-contamination during chicken preparation. Elevated fecal coliform counts were found in two of four ready-to-eat food samples collected from establishment A, and Bacillus cereus was detected in three. The outbreak strain of Salmonella was isolated from one patient's leftover chicken. Establishing automated spatiotemporal cluster detection analyses for salmonellosis and other reportable diseases could aid in the detection of geographically focused, community-acquired outbreaks even before laboratory subtyping results become available.

Synergistically coupling membrane electrochemical reactor with Fenton process to enhance landfill leachate treatment.

Landfill leachate is challenging to treat due to its complex composition. Advanced oxidation processes such as Fenton process can be effective to treat leachate. Herein, a previously developed membrane electrochemical reactor (MER) was coupled with Fenton oxidation through providing synergistic benefits with the low solution pH, reduced organics, and ammonia removal/recovery. This two-stage coupled system reduced the leachate COD by 88%, much higher than that from the standalone Fenton process treating raw leachate. In addition, the usage of chemical reagents has been greatly reduced. At a dimensionless oxidant dose of 1.0, the coupled MER-Fenton system reduced the consumption of both FeSO4⋅7H2O and H2O2 by 39%, H2SO4 by 100%, and NaOH by 55%. Consequently, the sludge production was reduced by 51% in weight and 12% in volume. Despite electricity consumption by the MER, the coupled system cost $4.76 per m3 leachate less than the standalone Fenton treatment. More notably, direct Fenton oxidation removed only 21% of ammonia; in comparison the MER-Fenton system removed ammonia by 98% with the possibility for recovery at a rate of 30.6-55.2 kg N m-3 reactor d-1. Those results demonstrate that coupling MER with Fenton process could mitigate some inherent drawbacks of Fenton oxidation such as ineffective ammonia removal, high acid and chemical reagents dose requirements, and a large amount of sludge generation. This system may be moved towards practical applications by addressing a few challenges such as using renewable energy to power MER.

Formation of disinfection byproducts during Fenton's oxidation of chloride-rich landfill leachate.

Because of the production of chlorine species in leachate during Fenton's oxidation, harmful disinfection byproducts (DBP) can be formed but this has not been well studied before. Herein, we have investigated five classes of DBP: trihalomethanes, haloacetic acids, haloacetonitriles, haloketones, and halonitromethanes during Fenton's oxidation of landfill leachates. The results show that the DBP concentration increased with the increase of [H2O2]: [Cl-] ratio due to the increased concentration of chlorine species. The highest total DBP concentration was 4860 µg L-1 at [H2O2]: [Cl-] = 4.0 and the lowest was 84 µg L-1 at [H2O2]: [Cl-] = 0.25. Both the DBP concentration and DBP toxicity increased with the increase of the [H2O2]: [Fe2+] ratio, because of the increased concentration and lifetime of the chlorine species. Most of the DBP were formed during the first minute of the reaction and stayed stable up to 3 h, indicating that DBP may not be preferred targets of hydroxyl radicals in the presence of a large amount of organics. In most cases, trihalomethanes dominated the DBP concentration, while haloacetonitriles dominated the total additive toxicity. This study has provided important implications to understand DBP formation during Fenton's oxidation.

Using cerium chloride to control soluble orthophosphate concentration and improve the dewaterability of sludge: Part I. Mechanistic understanding.

Cerium chloride (CeCl3 ), being a superior orthophosphate (OP) precipitant, was found to be able to significantly improve sludge dewaterability in terms of sludge cake dryness and capillary suction time. In order to offer insights into the mechanism behind OP removal associated dewaterability improvement, the change in sludge specific resistance to filtration (SRF), compressibility (K), and bound water contents (Ub ) in response to CeCl3 and CePO4 addition at the two cationic polymer doses was mathematically simulated. Results showed that 29.8 g/kg dry solid CePO4 addition was able to decrease the SRF by 52%, decrease the Ub by 42%, and reduce the K by 18%. Importantly, CeCl3 addition of equal cerium molarity showed even higher SRF and Ub reductions by 67% and 54%, respectively, but the same K reduction. A new theory depicting how the OP has outcompeted negatively charged sludge particles for cationic polymers is put forward in this study to interpret the effect of OP removal on sludge dewaterability improvement. PRACTITIONER POINTS: Efficient orthophosphate (OP) removal and sludge dewaterability improvement were achieved with CeCl3 addition. Both CePO4 precipitate and OP removal contributed to the improved dewaterability. Competition between OP and sludge particles for cationic polymers was explained.

Removal of landfill leachate ultraviolet quenching substances by electricity induced humic acid precipitation and electrooxidation in a membrane electrochemical reactor.

Persistent UV quenching substances (UVQS) in landfill leachate can affect the effectiveness of UV disinfection in domestic wastewater treatment systems when leachate is being co-treated. As a result, effective onsite leachate pre-treatment will have to be implemented to reduce the UV quenching capability. Herein, a membrane electrochemical reactor (MER) was developed and investigated for treating UV quenching organics contained in landfill leachate. Compared to a control reactor that did not have a membrane separator, the MER achieved significantly higher removals of both dissolved organic carbon (61.5 ±â€¯4.1%) and UV254nm absorbance (63.4 ±â€¯8.4%). This enhanced performance was likely due to the combined effects of humic acid precipitation and augmented oxidation of organics. The MER was able to remove 89.1 ±â€¯2.9% of total nitrogen from the leachate while recovering about 51% of the influent ammonia in the catholyte, in comparison to 38.1 ±â€¯4.4% of total nitrogen removal by the control reactor. The MER consumed significantly less electrical energy with specific energy consumption of 70.62 kWh kg-1 DOC or 33.03 kWh kg-1 sCOD, compared to that of the control reactor (211.8 kWh kg-1 DOC or 55.02 kWh kg-1 sCOD). A current density of 20 mA cm-2 was considered optimal in terms of both UVQS removal and energy efficiency. Consideration should be given to the spacing of electrodes to minimize internal resistance and also to avoid trapping of the produced gas bubbles. These results collectively suggest that the MER is a promising onsite pretreatment approach for landfill leachate and further exploration of this technology should be encouraged.

Effective depth controls the nitrate removal rates in a water supply reservoir with a high nitrate load.

The Occoquan Reservoir is part of an indirect potable reuse system where a water reclamation plant (WRP) discharges a nitrified product water to prevent the onset of anaerobic conditions in the bottom sediments during the summer months. The elongated narrow shape of the reservoir combined with water temperature gradients in the inlet results in density currents that enhance the transport of nitrate from the surface to the bottom waters. The morphology of the reservoir also causes a longitudinal change in the ratio of water volume to sediment area, herein defined as the effective depth (ZED). Field observations revealed that first-order nitrate removal rate coefficients (k) varied inversely with ZED, suggesting that the upper reaches of the reservoir have a higher potential for nitrate removal compared to the areas closer to the dam. A similar relationship between k (d-1) and ZED was confirmed during laboratory experiments. Differences in k values were attributed mainly to the change in the nitrate supply rate as a result of the increase in water volume flowing over a specific sediment area, which limited nitrate transport to the sediments. The low variability found between the mass transfer coefficients for nitrate (Coefficient of Variation = 0.25) suggested a nearly constant biotic nitrogen removal and confirmed that k values were mainly affected by changes in ZED. Finally, similarities in k values between field and laboratory samples with similar ZED values suggested that different segments of natural systems may be properly downscaled to laboratory-sized configurations for analytical purposes by means of the ZED concept.

Reduction of reagent requirements and sludge generation in Fenton's oxidation of landfill leachate by synergistically incorporating forward osmosis and humic acid recovery.

Applications of Fenton's oxidation of landfill leachate is limited by both high reagent requirements and a large amount of sludge generation. To address those issues, forward osmosis (FO) and humic acid (HA) recovery were incorporated with Fenton's treatment. In the FO, leachate was concentrated by 3.2 times in 10 hours using a 5-M NaCl draw solution. The HA recovery increased from 1.86 to 2.45 g L-1 at pH 2 after FO concentration, mainly because of the replacement of O in the HA structure by other inorganics (i.e., Cl, Na, K) with higher molecular weights. Due to the movement of alkalinity causing species (i.e., HCO3-, CO32-) to the draw side driven by a concentration gradient, the H2SO4 requirement per g of recovered HA and per g of removed COD decreased by 46.4% and 17.1%, respectively. The HA recovery also decreased sludge generation by 30%. At a dimensionless oxidant dose of 0.5, the proposed system reduced the overall requirement of H2SO4 by 25.2%, NaOH by 34.6%, and both FeSO4.7H2O and H2O2 by 35%, compared to the standalone Fenton's treatment of raw leachate. Those results have demonstrated that the proposed system could greatly decrease the leachate volume, lower the reagent requirements, and reduce the sludge production towards sustainable leachate treatment.

A review of landfill leachate induced ultraviolet quenching substances: Sources, characteristics, and treatment.

Landfill leachate contains extremely diverse mixtures of pollutants and thus requires appropriate treatment before discharge. Co-treatment of landfill leachate with sewage in wastewater treatment plants is a common approach because of low cost and convenience. However, some recalcitrant organic compounds in leachate can escape biological treatment processes, lower the UV transmittance of waste streams due to their UV-quenching properties, and interfere with the associated disinfection efficacy. Thus, the leachate UV quenching substances (UVQS) must be removed or reduced to a level that UV disinfection is not strongly affected. UVQS consist of three major fractions, humic acids, fulvic acids and hydrophilics, each of which has distinct characteristics and behaviors during treatment. The purpose of this review is to provide a synthesis of the state of the science regarding UVQS and possible treatment approaches. In general, chemical, electrochemical, and physical treatments are more effective than biological treatments, but also costlier. Integration of multiple treatment methods to target the removal of different fractions of UVQS can aid in optimizing treatment. The importance of UVQS effects on wastewater treatment should be better recognized and understood with implemented regulations and improved research and treatment practice.

Preliminary investigation of quorum quenching effects on sludge quantity and quality of activated sludge process.

The Quorum Sensing (QS) system has attracted the interest of researchers as a cell-cell communication system. In activated sludge processes, the production of extracellular polymeric substances (EPS), biofilms and floc formation are regulated by the QS system. Hence, disruption of the QS system, called Quorum Quenching (QQ), could have a significant effect on the quality and quantity of excess sludge. In the present research, the quorum quenching bacteria, Rhodococcus sp. BH4 was used as a quorum quencher and was entrapped in an alginate structure (QQ beads). Three separate sequential batch reactors (SBR) were constructed and operated as a control reactor, a Low-QQ reactor (containing 150 QQ beads), and a High-QQ reactor (containing 600 QQ beads). Results indicated that the presence of QQ beads in the aeration reactor leads to a decrease in EPS content and mean floc particle size in the both Low-QQ and High-QQ reactors. The eukaryotic community was changed significantly so that the QS disruption caused an enhancement in microbial predation. The presence of QQ beads also led to a 16 and a 26% decrease in the Yobs coefficient within the Low-QQ and High-QQ reactors, respectively. Findings of this research revealed a new application of the QQ system in the activated sludge process, but additional studies are needed.

Kinetic modeling of the effect of solids retention time on methanethiol dynamics in anaerobic digestion.

The highly volatile methanethiol (MT) with an extremely low odor threshold and distinctive putrid smell is often identified as a major odorous compound generated under anaerobic conditions. As an intermediate compound in the course of anaerobic digestion, the extent of MT emission is closely related to the time of anaerobic reaction. In this study, lab-scale anaerobic digesters were operated at solids retention time (SRTs) of 15, 20, 25, 30, 40 and 50 days to investigate the effect of SRT on MT emission. The experimental results demonstrated a bell-shaped curve of MT emission versus SRT with a peak around 20 days SRT. In order to understand this SRT effect, a kinetic model was developed to describe MT production and utilization dynamics in the course of anaerobic digestion and calibrated with the experimental results collected from this study. The model outcome revealed that the high protein content in the feed sludge together with the large maintenance coefficient of MT fermenters are responsible for the peak MT emission emergence in the range of typical SRT used for anaerobic digestion. A further analysis of the kinetic model shows that it can be extensively simplified with reasonable approximation to a form that anaerobic digestion practitioners could easily use to predict the MT and SRT relationship.

Thermal Degradation of Long Chain Fatty Acids.

The thermal hydrolysis of saturated (C16:0 and C18:0) and unsaturated fatty acids (C16:1, C18:1, and C18:2) was investigated at 90 °C to 160 °C for 30 min and 8 h durations. Hydrolysis efficiencies were calculated based on mass yield (i.e., mg/g parent compound), which accounted for all C2-C24 by-products. Very little degradation (less than 1%) of long chain fatty acids (LCFAs) was observed from 30 min thermal hydrolysis. At 140 and 160 °C for 8 h, saturated fatty acids degraded uniformly to C2 to C14. Saturated fatty acids tended to convert to alkanes (1.5-2.0% of total fatty acids) instead of fatty acids (8 h). Thermal hydrolysis did not significantly affect unsaturated LCFA degradation at any duration. The unsaturated by-products seen were the result of cleavage at the allylic or vinylic positions. Thermal hydrolysis of LCFAs with digested sludge was investigated. The amount of VFAs and LCFAs in primary and secondary sludge was increased at 140 and 160 °C as a result of lipid degradation in the sludge mixture. Thermal hydrolysis of fatty acids with different catalysts was also investigated. Whereas saturated LCFAs were stable under all catalytic conditions, unsaturated LCFAs were nearly completely degraded when hydrolyzed with hydrogen peroxide and activated carbon or copper sulfate.

Enhancing forward osmosis water recovery from landfill leachate by desalinating brine and recovering ammonia in a microbial desalination cell.

In this work, a microbial desalination cell (MDC) was employed to desalinate the FO treated leachate for reduction of both salinity and chemical oxygen demand (COD). The FO recovered 51.5% water from a raw leachate and the recovery increased to 83.5% from the concentrated leachate after desalination in the MDC fed with either acetate or another leachate as an electron source and at a different hydraulic retention time (HRT). Easily-degraded substrate like acetate and a long HRT resulted in a low conductivity desalinated effluent. Ammonia was also recovered in the MDC cathode with a recovery efficiency varying from 11 to 64%, affected by current generation and HRT. Significant COD reduction, as high as 65.4%, was observed in the desalination chamber and attributed to the decrease of both organic and inorganic compounds via diffusion and electricity-driven movement.

Two-stage Anaerobic Membrane Bioreactor (AnMBR) system to reduce UV absorbance in landfill leachates.

Landfill leachate typically contains UV-quenching organics, which hinder disinfection at POTWs. This study tested a 2-stage submerged AnMBR for the degradation of UV-absorbing compounds in landfill leachate. Leachate was treated in a thermophilic reactor (55 ±â€¯2 °C) followed by a mesophilic AnMBR (37 ±â€¯1 °C), with HRTs of 25 ±â€¯5 days and 40 ±â€¯5 days respectively. Solids were not wasted, in order to promote biomass accumulation. COD, Organic carbon, and UV254 absorbance were monitored over 13 months of operation. Known UV-quenching compounds, including humic acids, fulvic acids and hydrophilic matter, were reduced by 55%. Molecular weight distribution analyses revealed that the thermophilic reactor hydrolyzed organic carbon >100 KDa into smaller fractions, which were removed in the AnMBR. The system consistently removed 50% of the total UV absorbance. This promising, new enhanced biological process may provide landfills with a feasible pretreatment alternative to expensive chemical oxidation or RO processes before discharging leachate into sewers.

Effect of High Strength Food Wastes on Anaerobic Codigestion of Sewage Sludge.

Anaerobic codigestion has been practiced at water resource recovery facilities to increase methane production, but the impact of many variables is still not well understood. In this study, the feasibility of codigesting fats, oils, and grease (FOG), and other high strength wastes (HSWs) with municipal sewage sludge was investigated. Four laboratory-scale digesters were operated at a working volume of 9.75 L, 15 days solids retention time (SRT), and at a temperature of 37 °C. Wastes including whey (cheese), juice, grease trap waste (GTW), and dissolved air flotation waste (DAF), along with municipal sewage sludge, were fed to the digesters in varying amounts. The addition of HSWs led to higher methane production at lower organic loadings. However, at higher organic loadings, the GTW appeared to be toxic to methanogens, leading to a decrease in digester pH and biogas production, and an accumulation of volatile fatty acids within the digester.

Reverse flexing as a physical/mechanical treatment to mitigate fouling of fine bubble diffusers.

Achieving energy neutrality has shifted focus towards aeration system optimization, due to the high energy consumption of aeration processes in modern advanced wastewater treatment plants. A study on fine bubble diffuser fouling and mitigation, quantified by dynamic wet pressure (DWP), oxygen transfer efficiency and alpha was carried out in Blue Plains, Washington, DC. Four polyurethane fine bubble diffusers were installed in a pilot reactor column fed with high rate activated sludge from a full scale system. A mechanical cleaning method, reverse flexing (RF), was used to treat two diffusers (RF1, RF2), while two diffusers were kept as a control (i.e., no reverse flexing). There was a 45% increase in DWP of the control diffuser after 17 months of operation, an indication of fouling. RF treated diffusers (RF1 and RF2) did not show significant increase in DWP, and in comparison to the control diffuser prevented about 35% increase in DWP. Hence, reverse flexing potentially saves blower energy, by reducing the pressure burden on the air blower which increases blower energy requirement. However, no significant impact of the RF treatment in preventing a decrease in alpha-fouling (αF) of the fine pore diffusers, over time in operation was observed.

Simultaneous energy generation and UV quencher removal from landfill leachate using a microbial fuel cell.

The presence of UV quenching compounds in landfill leachate can negatively affect UV disinfection in a wastewater treatment plant when leachate is co-treated. Herein, a microbial fuel cell (MFC) was investigated to remove UV quenchers from a landfill leachate with simultaneous bioelectricity generation. The key operating parameters including hydraulic retention time (HRT), anolyte recirculation rate, and external resistance were systematically studied to maximize energy recovery and UV absorbance reduction. It was found that nearly 50% UV absorbance was reduced under a condition of HRT 40 days, continuous anolyte recirculation, and 10 Ω external resistance. Further analysis showed a total reduction of organics by 75.3%, including the reduction of humic acids, fulvic acids, and hydrophilic fraction concentration as TOC. The MFC consumed 0.056 kWh m-3 by its pump system for recirculation and oxygen supply. A reduced HRT of 20 days with periodical anode recirculation (1 hour in every 24 hours) and 39 Ω external resistance (equal to the internal resistance of the MFC) resulted in the highest net energy of 0.123 kWh m-3. Granular activated carbon (GAC) was used as an effective post-treatment step and could achieve 89.1% UV absorbance reduction with 40 g L-1. The combined MFC and GAC treatment could reduce 92.9% of the UV absorbance and remove 89.7% of the UV quenchers. The results of this study would encourage further exploration of using MFCs as an energy-efficient method for removing UV quenchers from landfill leachate.