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1.
J Dent Res ; 92(3): 235-40, 2013 Mar.
Article in English | MEDLINE | ID: mdl-23300185

ABSTRACT

This report describes the use of a novel model of multispecies biofilms to stimulate profiles of cytokines/chemokines from oral epithelial cells that contribute to local inflammation in the periodontium. Streptococcus gordonii (Sg)/S. oralis (So)/S. sanguinis (Ss) and Sg/Fusobacterium nucleatum (Fn)/Porphyromonas gingivalis (Pg) biofilms elicited significantly elevated levels of IL-1α and showed synergistic stimulatory activity compared with an additive effect of the 3 individual bacteria. Only the Sg/Actinomyces naeslundii (An)/Fn multispecies biofilms elicited IL-6 levels above those of control. IL-8 was a primary response to the Sg/An/Fn biofilms, albeit the level was not enhanced compared with a predicted composite level from the monospecies challenges. These results represent some of the first data documenting alterations in profiles of oral epithelial cell responses to multispecies biofilms.


Subject(s)
Biofilms , Cytokines/biosynthesis , Host-Pathogen Interactions , Microbial Consortia/physiology , Mouth Mucosa/microbiology , Periodontitis/microbiology , Biofilms/growth & development , Cell Line, Transformed , Epithelial Cells/microbiology , Fusobacterium nucleatum/physiology , Humans , Interleukin-1alpha/biosynthesis , Interleukin-6/biosynthesis , Mouth Mucosa/cytology , Mouth Mucosa/metabolism , Porphyromonas gingivalis/physiology , Streptococcus/physiology
2.
Int J Antimicrob Agents ; 38(6): 530-3, 2011 Dec.
Article in English | MEDLINE | ID: mdl-21920706

ABSTRACT

WLBU2 is a peptide antibiotic designed for broad antimicrobial activity, including bacteria associated with periodontal disease. Although periodontitis is associated with various systemic conditions, ranging from cardiovascular disease to preterm birth, local therapy is needed to treat the source of infection. Biodegradable polymers are often used to control locally the amount and rate of delivery of drugs. In the present study, a bioerodible association polymer comprising cellulose acetate phthalate (CAP) and Pluronic F-127 (PF-127) was explored for its interaction with WLBU2. The intrinsic antimicrobial activity of CAP/PF-127 and the combined effects of the polymer and WLBU2 were examined using Streptococcus gordonii, a species involved in early colonisation of tooth surfaces. The polymer blend alone had dose-dependent bacteriostatic properties, resulting in a ≥ 2 log decrease in colonies at the highest concentrations tested, possibly due to the hydrophobicity of CAP disrupting the surface of bacteria. When WLBU2 was combined with CAP/PF-127, an apparent binding of peptide to polymer significantly decreased the activity compared with free WLBU2, which functions like other cationic peptides by destabilising the bacterial membrane. Formulation with sucrose as an excipient, which reduced the interaction between WLBU2 and polymer, restored the bactericidal activity of the peptide antibiotic as reflected by a > 3 log decrease in S. gordonii. WLBU2 can be locally delivered using CAP/PF-127 as a release vehicle, with the peptide's bactericidal activity dominating the polymer's bacteriostatic effect.


Subject(s)
Anti-Bacterial Agents/pharmacology , Antimicrobial Cationic Peptides/pharmacology , Cellulose/analogs & derivatives , Drug Carriers/pharmacology , Poloxamer/pharmacology , Polymers/pharmacology , Cellulose/chemistry , Cellulose/pharmacology , Drug Carriers/chemistry , Escherichia coli/drug effects , Escherichia coli/growth & development , Humans , Microbial Sensitivity Tests , Poloxamer/chemistry , Polymers/chemistry , Streptococcus gordonii/drug effects , Streptococcus gordonii/growth & development
3.
Clin Vaccine Immunol ; 18(10): 1770-2, 2011 Oct.
Article in English | MEDLINE | ID: mdl-21832101

ABSTRACT

An in vitro model of bacterial biofilms on rigid gas-permeable contact lenses (RGPLs) was developed to challenge oral epithelial cells. This novel model provided seminal data on oral biofilm-host cell interactions, and with selected bacteria, the biofilms were more effective than their planktonic counterparts at stimulating host cell responses.


Subject(s)
Bacteria/immunology , Biofilms , Epithelial Cells/immunology , Epithelial Cells/microbiology , Interleukin-8/metabolism , Mouth Mucosa/immunology , Mouth Mucosa/microbiology , Host-Pathogen Interactions
4.
Open Dent J ; 2: 49-55, 2008.
Article in English | MEDLINE | ID: mdl-19088882

ABSTRACT

Quantitative PCR (qPCR) has recently been used to quantify microorganisms in complex communities, including dental plaque biofilms. However, there is variability in the qPCR protocols being used. This study was designed to evaluate the validity of two of these variables with the intent of developing a more standardized qPCR protocol. The two variables evaluated were (1) the use of DNA content versus actual cell counts to estimate bacterial numbers in mixed plaque samples and (2) the effectiveness of three different universal primers versus species specific primers in amplifying specific target pathogens in these samples. Results lead to the development of a standardized protocol that was shown to be highly reproducible as demonstrated by low coefficients of variation. The results also confirmed that this standardized qPCR protocol can be used as a sensitive method for quantifying specific bacterial species in human plaque samples.

5.
J Dent Res ; 87(10): 928-31, 2008 Oct.
Article in English | MEDLINE | ID: mdl-18809745

ABSTRACT

We have recently demonstrated that extracorporeal shock-wave therapy (ESWT) is effective in promoting the healing of dermal wounds and in regenerating alveolar bone lost through periodontal disease. The objective of the present study was to determine any antibacterial effect of ESWT on oral bacteria. Monoculture suspensions of 6 bacterial species were treated with 100 to 500 pulses of ESWT at energy flux densities (EFD) of 0.12 mJ/mm(2), 0.22 mJ/mm(2), and 0.3 mJ/mm(2). Following treatment, aliquots were plated for viability determination and compared with untreated controls. ESWT showed a significant microbicidal effect for Streptococcus mutans and an unencapsulated strain of Porphyromonas gingivalis following as few as 100 pulses at 0.3 mJ/mm(2) (p 0.05). These findings suggest that low-energy ESWT may be bactericidal for selected oral bacteria.


Subject(s)
Porphyromonas gingivalis/radiation effects , Radiation, Nonionizing , Streptococcus mutans/radiation effects , Actinomyces/radiation effects , Analysis of Variance , Bacterial Adhesion/radiation effects , Colony Count, Microbial , Fusobacterium nucleatum/radiation effects , Microbial Viability/radiation effects , Radiation Dosage , Staphylococcus aureus/radiation effects , Statistics, Nonparametric
6.
J Periodontal Res ; 43(5): 500-7, 2008 Oct.
Article in English | MEDLINE | ID: mdl-18565132

ABSTRACT

BACKGROUND AND OBJECTIVE: Dietary manipulation, including caloric restriction, has been shown to impact host response capabilities significantly, particularly in association with aging. This investigation compared systemic inflammatory and immune-response molecules in rhesus monkeys (Macaca mulatta). MATERIAL AND METHODS: Monkeys on continuous long-term calorie-restricted diets and a matched group of animals on a control ad libitum diet, were examined for systemic response profiles including the effects of both gender and aging. RESULTS: The results demonstrated that haptoglobin and alpha1-antiglycoprotein levels were elevated in the serum of male monkeys. Serum IgG responses to Campylobacter rectus, Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis were significantly elevated in female monkeys. While only the antibody to Fusobacterium nucleatum was significantly affected by the calorie-restricted diet in female monkeys, antibody levels to Prevotella intermedia, C. rectus and Treponema denticola demonstrated a similar trend. CONCLUSION: In this investigation, only certain serum antibody levels were influenced by the age of male animals, which was seemingly related to increasing clinical disease in this gender. More generally, analytes were modulated by gender and/or diet in this oral model system of mucosal microbial challenge.


Subject(s)
Aging/physiology , Caloric Restriction , Inflammation/immunology , Sex Factors , Acute-Phase Proteins/analysis , Animals , Antibodies, Bacterial/blood , Bacteria, Anaerobic/immunology , Female , Immunity, Mucosal/physiology , Immunoglobulin G/blood , Inflammation/blood , Linear Models , Macaca mulatta , Male , Periodontal Diseases/immunology , Periodontal Diseases/microbiology
7.
Microbiology (Reading) ; 147(Pt 11): 3027-35, 2001 Nov.
Article in English | MEDLINE | ID: mdl-11700353

ABSTRACT

Nine contiguous genes encoding a potential type IV secretion system have been identified in the chromosome of Actinobacillus actinomycetemcomitans strain VT747 and on a plasmid (pVT745) in strain VT745. Seven of these genes encode predicted proteins that share significant homology with type IV secretion proteins in Bordetella pertussis (ptl operon), Brucella melitensis biovar suis and Agrobacterium tumefaciens (virB operons), where they are involved in protein secretion, pathogen intracellular survival and multiplication, and DNA transport, respectively. Results of previous studies have demonstrated that pVT745 is a conjugative plasmid and that a secondary plasmid, pMMB67, can be mobilized from strain VT745. Given these results, it was hypothesized that (1) the type IV secretion genes on pVT745 are responsible for these two functions and (2) the type IV VT747 chromosomal genes also play a role in the transport of DNA. Wild-type and mutant strains of VT745 were evaluated for their conjugative abilities. Wild-type mating efficiency was 10(-6) transconjugants per donor, while the mutant strain yielded no transconjugants. Wild-type VT745 harbouring a co-resident plasmid, pMMB67, mobilized pMMB67 at a frequency of 10(-6), while VT747 was unable to mobilize this plasmid. These results support the hypothesis that the plasmid-encoded type IV secretion system on pVT745 is involved in DNA transport. However, the chromosomally encoded secretion system may not play a role in DNA transport in strain VT747. While the precise function of these chromosomal genes in strain VT747 has not been determined, Northern blot analyses demonstrated that these genes are expressed in both ACT: actinomycetemcomitans strains VT745 and VT747.


Subject(s)
Aggregatibacter actinomycetemcomitans/genetics , Bacterial Proteins/metabolism , Chromosomes, Bacterial , Plasmids , Aggregatibacter actinomycetemcomitans/physiology , Bacterial Proteins/genetics , Bacterial Proteins/physiology , Blotting, Northern , Conjugation, Genetic , Genes, Bacterial , Microbial Sensitivity Tests , Molecular Sequence Data , Mutation , Sequence Analysis, DNA
8.
J Bacteriol ; 183(5): 1585-94, 2001 Mar.
Article in English | MEDLINE | ID: mdl-11160089

ABSTRACT

The complete nucleotide sequence and genetic map of pVT745 are presented. The 25-kb plasmid was isolated from Actinobacillus actinomycetemcomitans, a periodontal pathogen. Two-thirds of the plasmid encode functions related to conjugation, replication, and replicon stability. Among potential gene products with a high degree of similarity to known proteins are those associated with plasmid conjugation. It was shown that pVT745 derivatives not only mobilized a coresident nontransmissible plasmid, pMMB67, but also mediated their own conjugative transfer to different A. actinomycetemcomitans strains. However, transfer of pVT745 derivatives from A. actinomycetemcomitans to Escherichia coli JM109 by conjugation was successful only when an E. coli origin of replication was present on the pVT745 construct. Surprisingly, 16 open reading frames encode products of unknown function. The plasmid contains a conserved replication region which belongs to the HAP (Haemophilus-Actinobacillus-Pasteurella) theta replicon family. However, its host range appears to be rather narrow compared to other members of this family. Sequences homologous to pVT745 have previously been detected in the chromosomes of numerous A. actinomycetemcomitans strains. The nature and origin of these homologs are discussed based on information derived from the nucleotide sequence.


Subject(s)
Aggregatibacter actinomycetemcomitans/genetics , Conjugation, Genetic/genetics , Plasmids/genetics , Aggregatibacter actinomycetemcomitans/metabolism , Base Sequence , Escherichia coli/genetics , Humans , Molecular Sequence Data , Open Reading Frames/genetics , Recombination, Genetic , Replication Origin/genetics , Sequence Analysis, DNA
9.
Microbiol Immunol ; 44(2): 143-8, 2000.
Article in English | MEDLINE | ID: mdl-10803501

ABSTRACT

Results of Southern blot analyses and polymerase chain reaction revealed that the Gram-negative pathogen, Actinobacillus actinomycetemcomitans, harbored DNA homologous to the secA gene of Escherichia coli. In E. coli, the secA gene product is essential for translocation of proteins across the inner membrane via the Sec system. This A. actinomycetemcomitans secA homolog was cloned and its nucleotide sequence determined. Amino acid sequence analysis of the cloned gene revealed significant homology to the SecA proteins of Haemophilus influenzae, E. coli, Caulobacter crescentus and Bacillus subtilis. Although the cloned gene did not complement a temperature sensitive mutation in the E. coli secA gene, strains harboring the cloned gene did produce a protein that cross-reacted with anti-SecA antibody. In addition, the cloned gene did restore sensitivity to sodium azide in an E. coli azide mutant. These data support the hypothesis that A. actinomycetemcomitans may use a system similar to the Sec system of E. coli to transport proteins across the cytoplasmic membrane, but suggest that the A. actinomycetemcomitans gene product may require genera-specific Sec proteins to complement some Sec mutations in E. coli.


Subject(s)
Adenosine Triphosphatases/genetics , Adenosine Triphosphatases/metabolism , Aggregatibacter actinomycetemcomitans/genetics , Carrier Proteins/genetics , Carrier Proteins/metabolism , Escherichia coli Proteins , Genes, Bacterial , Membrane Transport Proteins , Adenosine Triphosphatases/chemistry , Aggregatibacter actinomycetemcomitans/metabolism , Amino Acid Sequence , Bacterial Proteins/metabolism , Base Sequence , Blotting, Western , Carrier Proteins/chemistry , Cloning, Molecular , Escherichia coli/genetics , Escherichia coli/metabolism , Molecular Sequence Data , Polymerase Chain Reaction , SEC Translocation Channels , SecA Proteins , Sequence Analysis, DNA , Sequence Homology, Amino Acid
10.
Oral Microbiol Immunol ; 13(2): 124-8, 1998 Apr.
Article in English | MEDLINE | ID: mdl-9573804

ABSTRACT

Plasmid pVT745 is a 25.1-kb replicon isolated from Actinobacillus actinomycetemcomitans strain VT745. A previous report described the hybridization of pVT745 in 5 strain-specific patterns to chromosomal DNA from 15 other A. actinomycetemcomitans strains. However, pVT745 does not share homology with the chromosome of the strain from which it was isolated, VT745. It was hypothesized that the shared areas of homology might represent insertion sequence elements and/or transposons possibly encoding resistance to one or more antibiotics. An antibiogram of strain VT745 demonstrated that this strain was uniformly susceptible to all antibiotics examined. Because insertion sequence elements and transposons are mobile genetic elements, a series of cell passaging experiments, followed by Southern hybridization was conducted in a attempt to detect transposition of pVT745 homologous DNA within the chromosomes of several A. actinomycetemcomitans strains. The results of these experiments suggested stability of the homologous DNA both within the chromosome and on the plasmid. It was also possible that pVT745 represented a lysogenic bacteriophage. Phage induction experiments were conducted under conditions that induced a previously described A. actinomycetemcomitans lysogenic phage, but no phage could be induced from strain VT745. Attempts to obtain isolates of VT745 cured of pVT745 were also unsuccessful.


Subject(s)
Aggregatibacter actinomycetemcomitans/genetics , Plasmids/genetics , Aggregatibacter actinomycetemcomitans/drug effects , Anti-Bacterial Agents/antagonists & inhibitors , Anti-Bacterial Agents/pharmacology , Autoradiography , Blotting, Southern , DNA, Bacterial/genetics , DNA, Bacterial/isolation & purification , Drug Resistance, Microbial/genetics , Microbial Sensitivity Tests , Plasmids/isolation & purification , Replicon/genetics , Sequence Homology, Nucleic Acid
11.
Curr Opin Periodontol ; 3: 45-58, 1996.
Article in English | MEDLINE | ID: mdl-8624569

ABSTRACT

The group of periodontal diseases known as the early-onset periodontal diseases are defined by the age of onset of periodontal destruction, distribution of lesions, association of disease with specific microbial infections, and identification of characteristic alterations, in the host response. Significant progress has recently been made in our understanding of the etiology of juvenile and rapidly progressive periodontitis. Considerable evidence points to a familial pattern of disease; both localized and generalized forms of disease may be observed in the same family. The exact mode of inheritance remains unclear, and disease may be the result of a complex interplay between genetically determined alterations of the host response and a specific bacterial challenge. Both neutrophil function and immunoglobulin response are altered and appear to be characteristic of an immunologic hyperresponsiveness. Bacterial colonization by Actinobacillus actinomycetemcomitans (serotype b) and/or Porphyromonas gingivalis appears to be the primary initiator of disease. Evidence suggests that lesion distribution may be a function of the nature of the infecting agent and the characteristics of the immune response.


Subject(s)
Aggressive Periodontitis/etiology , Periodontitis/etiology , Adolescent , Adult , Aggregatibacter actinomycetemcomitans/pathogenicity , Aggressive Periodontitis/genetics , Aggressive Periodontitis/immunology , Aggressive Periodontitis/microbiology , Antibodies, Bacterial/blood , Chemotaxis, Leukocyte , Child , Child, Preschool , Disease Susceptibility/immunology , Genetic Predisposition to Disease , Humans , Neutrophils/immunology , Periodontitis/genetics , Periodontitis/immunology , Periodontitis/microbiology , Porphyromonas gingivalis/pathogenicity , Risk Factors
12.
Plasmid ; 31(1): 31-9, 1994 Jan.
Article in English | MEDLINE | ID: mdl-8171123

ABSTRACT

A 25.1-kb plasmid, pVT745, was isolated from Actinobacillus actinomycetemcomitans strain VT745. This plasmid hybridized under stringent conditions to chromosomal DNA from 15 A. actinomycetemcomitans isolates obtained from geographically diverse regions of the United States. Southern blot analyses of HincII-digested A. actinomycetemcomitans genomic DNA revealed five strain-specific patterns of hybridization, which clearly indicated that intact pVT745 was not inserted into any of the genomes. Plasmid pVT745 was digested with BamHI and PstI into three, non-overlapping fragments of approximately 7.0, 8.0, and 10.0 kb. The fragments were cloned in Escherichia coli on the low copy number vector pGB2. Although these three fragments exhibited no cross-hybridization, genomic DNA from isolates representing each of the strain-specific patterns hybridized with two or three of the cloned fragments. The results suggest that two or more unique sequences within pVT745 also may be present in genomic DNA obtained from various strains of A. actinomycetemcomitans.


Subject(s)
Aggregatibacter actinomycetemcomitans/genetics , Plasmids/isolation & purification , Cloning, Molecular , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Bacterial/isolation & purification , Escherichia coli , Plasmids/chemistry , Restriction Mapping , Sequence Homology, Nucleic Acid
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