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J Clin Microbiol ; 40(8): 3100-3, 2002 Aug.
Article in English | MEDLINE | ID: mdl-12149392

ABSTRACT

We report the first case of Helicobacter sp. osteomyelitis in an immunocompetent child. The infection was diagnosed by broad-range 16S PCR followed by sequencing of the resulting amplicon. All other microbiological investigations proved negative. This case highlights the importance of molecular methods in the diagnosis of unsuspected etiological agents and the potential role of Helicobacter sp. in human infection.


Subject(s)
Helicobacter Infections/diagnosis , Helicobacter/isolation & purification , Immunocompetence , Osteomyelitis/diagnosis , Polymerase Chain Reaction/methods , RNA, Ribosomal, 16S/genetics , Bacterial Typing Techniques , Child , Child, Preschool , Chronic Disease , DNA, Ribosomal/analysis , Female , Helicobacter/genetics , Helicobacter Infections/microbiology , Humans , Molecular Sequence Data , Osteomyelitis/microbiology , Sequence Analysis, DNA
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